Abstract
The chicken embryo has been well used in studies of the developmental process, and during development sphingosine and sphingosine 1-phosphate (So1P) are considered critical mediators of cell death and survival. In this study, we compared the sphingolipid contents of chicken embryos during the early embryonic development period from day 3 to day 6. HPLC analyses of sphingosine and So1P in chicken embryos revealed that sphingosine levels were greatly reduced on day 4 whereas So1P levels were not significantly changed. Sphingosine kinase (Sphk) activities, which require sphingosine as substrate to produce So1P, were also greatly reduced on day 4. Collectively, we found sphingosine levels and Sphk activities, but not So1 P levels are changed in early stage of chicken embryos development.
Similar content being viewed by others
References
Jin, Y.X., Yoo, H.S., Kihara, A., Choi, C.H., Oh, S., Moon, D.C., Igarashi, Y., and Lee, Y.M., Sphingosine kinase assay system with fluorescent detection in high performance liquid chromatography.Arch. Pharm. Res., 29, 1049–1054 (2006).
Kim, D.H., Yoo, H.S., Lee, Y.M., Kie, J.H., Jang, S., and Oh, S., Elevation of sphinganine 1-phosphate as a predictive biomarker for fumonisin exposure and toxicity in mice.J. Toxicol. Environ. Health A., 69, 2071–2082 (2006).
Kim, J. I., Jo, E. J., Lee, H. Y., Cha, M. S., Min, J. K., Choi, C. H., Lee, Y. M., Choi, Y. A., Baek, S. H., Ryu, S. H., Lee, K. S., Kwak, J. Y., and Bae, Y. S., Sphingosine 1-phosphate in amniotic fluid modulates cyclooxygenase-2 expression in human amnion-derived WISH cells.J. Biol. Chem., 278, 31731–31736 (2003).
Kupperman, E., An, S., Osborne, N., Waldron, S., and Stainier, D. Y., A sphingosine-1-phosphate receptor regulates cell migration during vertebrate heart development.Nature, 406, 192–195 (2000)
Lee, W. J., Yoo, H. S., Suh, P. G., Oh, S., Lim, J. S., and Lee, Y. M., Sphingosine mediates FTY720-induced apoptosis in LLC-PK1 cells.Exp. Mol. Med., 36, 420–427 (2004).
Liu, Y., Wada, R., Yamashita, T., Mi, Y., Deng, C. X., Hobson, J. P., Rosenfeldt, H. M., Nava, V. E., Chae, S. S., Lee, M. J., Liu, C. H., Hla, T., Spiegel, S., and Proia, R. L., Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation.J Clin Invest., 106, 951–961 (2000).
Martinsen, B. J., Reference guide to the stages of chick heart embryology.Dev. Dyn., 233, 1217–1237 (2005).
Maceyka, M., Payne, S. G., Milstien, S., and Spiegel, S., Sphingosine kinase, sphingosine-1-phosphate, and apoptosis.Biochim. Biophys. Acta., 1585, 193–201 (2002).
Min, J. K., Yoo, H. S., Lee, E. Y., Lee, W. J., and Lee, Y. M., Simultaneous quantitative analysis of sphingoid base 1- phosphates in biological samples by o-phthalaldehyde precolumn derivatization after dephosphorylation with alkaline phosphatase.Anal. Biochem., 303, 167–175 (2002).
Murphy, M. J. and Clark, N. B., The avian embryo as a model for early developmental endocrinology.J. Exp. Zool. Suppl., 4, 177–180 (1990).
Oskouian, B. and Saba, J D., Death and taxis: what non- mammalian models tell us about sphingosine-1-phosphate.Semin. Cell Dev. Biol., 15, 529–540 (2004).
Patan, S., Vasculogenesis and angiogenesis as mechanisms of vascular network formation, growth and remodeling.J. Neurooncol., 50, 1–15 (2000).
Ryu, Y., Takuwa, N., Sugimoto, N., Sakurada, S., Usui, S., Okamoto, H., Matsui, O., and Takuwa, Y., Sphingosine-1- phosphate, a platelet-derived lysophospholipid mediator, negatively regulates cellular Rac activity and cell migration in vascular smooth muscle cells.Circ. Res., 90, 325–332 (2002).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Choi, CH., Jeong, JS., Yoo, Bi. et al. Sphingosine 1-Phosphate and Sphingosine Kinase Activity during Chicken Embryonic Development. Arch Pharm Res 30, 502–506 (2007). https://doi.org/10.1007/BF02980226
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02980226