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Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken

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Abstract

We have identified DNA polymorphisms in the gene of insulin-like growth factor 2 by PCR-SSCP in a resource population, which was generated by Silky reciprocally crossing to Broilers. A C →G mutation was detected in the exon 2 (at position 71) by sequencing. This single nucleotide polymorphism (SNP) was found to be associated with production traits. Chicken with BB genotype showed more chest angle width but less 3 week body weight and glandular stomach weight than chicken with AA genotype (P<0.05); while the heterozygote (AB genotype) chicken had more abdominal fat weight, eviscerated yield with giblet than AA homozygote chicken. Further analysis showed that there were different genetic effects on some traits between heterozygote AB (paternal allele given first) and heterozygote BA: chickens with genotype BA had more birth weight and breast weight but less abdominal fat weight than chickens with genotype AB (P<0.05), which could be hypothetically contributed by genome imprinting. Therefore, Silky chickens were selected for production of heterozygotes to confirm whether IGF2 locus was imprinting. Progeny from heterozygote × homozygote reciprocal cross was assayed for expression after the genotype was determined. The transcription of IGF2 was detected by RT-PCR-SSCP. IGF2 gene was expressed bialleleically in 1-day-old neonatal liver and 90-day-old liver, kidney, heart, and muscle of both heterozygote AB and BA chickens. Therefore, IGF2 was not an imprinting gene in chicken. The different genetic effects between the heterozygote AB and BA remain to be elucidated.

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Abbreviations

W0:

weight of birth

AF:

the abdominal fat weight

AFR:

abdominal fat rate

BW:

breast weight

BWR:

breast weight ration

SNP:

single nucleotide polymorphism

IGF2:

insulin-like growth factor 2

SSCP:

single strand conformation polymorphism

RT:

reverse transcription

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, 100094, Beijing, China

    Genyu Wang, Bingxue Yan, Xiaoxiang Hu & Ning Li

  2. Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100094, Beijing, China

    Xuemei Deng & Changlü Li

Authors
  1. Genyu Wang
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  2. Bingxue Yan
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  3. Xuemei Deng
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  4. Changlü Li
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  5. Xiaoxiang Hu
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  6. Ning Li
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Correspondence to Ning Li.

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Wang, G., Yan, B., Deng, X. et al. Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken. Sci. China Ser. C.-Life Sci. 48, 187–194 (2005). https://doi.org/10.1007/BF02879672

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  • DOI: https://doi.org/10.1007/BF02879672

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