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Quantitative determination of non-sulfated bile acids in the serum of patients with hepatobiliary diseases by mass fragmentography

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Summary

Individual non-sulfated bile acids in the serum of 65 patients with hepatobiliary diseases were quantitated by mass fragmentography. Serum with deuterium labeled deoxycholic acid as an internal standard was hydrolyzed with strong alkali, extracted with ether after acidification under cooling, and quantitated by mass fragmentography as the hexafluoroisopropyl-trifluoroacetyl derivatives. In obstructive jaundice, the ratio of cholic to chenodeoxycholic acid was significantly higher than others. Cholic or chenodeoxycholic acid levels were correlated with total bilirubin levels in obstructive jaundice and acute hepatitis. Lithocholic acid value was independent of the degree of liver injury. Total bile acid value was helpful in estimating the extent of liver cell injury and cholestasis, and these two pathological conditions can be distinguished to some extent by cholic to chenodeoxychoic acid ratio.

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Authors and Affiliations

  1. The Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, 113, Tokyo, Japan

    Hajime Takikawa M.D.

  2. Second Department of Surgery, Faculty of Medicine, University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, 113, Tokyo, Japan

    Tomoe Beppu M.D. & Tatsuo Wada M.D.

  3. Department of Biochemistry, Faculty of Medicine, University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, 113, Tokyo, Japan

    Yousuke Seyama M.D.

Authors
  1. Hajime Takikawa M.D.
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  2. Tomoe Beppu M.D.
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  3. Yousuke Seyama M.D.
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  4. Tatsuo Wada M.D.
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Additional information

This study was supported by a Grant-in-Aid for Scientific Research (A) from the Ministry of Education, Science and Culture of Japan (No. 57440062).

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Takikawa, H., Beppu, T., Seyama, Y. et al. Quantitative determination of non-sulfated bile acids in the serum of patients with hepatobiliary diseases by mass fragmentography. Gastroenterol Jpn 18, 246–251 (1983). https://doi.org/10.1007/BF02774967

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  • DOI: https://doi.org/10.1007/BF02774967

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