Summary
HLA-DR antigens in small and large intestinal epithelia were examined in Crohn’s disease (CD). Seventy-two biopsy specimens (10 from the duodenum, 18 from the terminal ileum, and 44 from various sites of the large intestine) were obtained from 5 patients with CD chronologically; before treatment, during elemental diet (ED) treatment, and during home elemental enterai hyperalimentation (HEEH). HLA-DR antigens on the intestinal epithelia were identified by the indirect immunoperoxidase staining method using two mouse anti-HLA-DR monoclonal antibodies. In the small intestine, HLA-DR antigens were expressed differently from normal controls in macroscopically or microscopically pathological areas, but not in macro-or microscopically normal areas. In the large intestine, when the disease was in full vigor before treatment, HLA-DR antigens were always aberrantly expressed even in the macroscopically uninvolved areas, as well as in macroscopically involved areas. However, when the disease was suppressed after ED followed by HEEH treatment, the antigens were not always expressed in the macroscopically uninvolved areas, or in the macroscopically involved areas. These findings suggest that HLA-DR antigens are not merely expressed as a non-specific response to inflammation, but may be expressed as a primary change which results in macroscopic lesions. The possible role of HLA-DR antigens in the immunological mechanism in CD is proposed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ogasawara K, Kojima H, Ikeda H, et al: A study on class II antigens involved in the T cell proliferative responses to PPD using cross-reacting monoclonal antibodies in human and murine system. Immunobiol 1986;171:112–124
Wiman K, Curman B, Forsum U, et al: Occurrence of la antigens on tissues of non-lymphoid origin. Nature 1978;276:711–713
Natali PG, Martino CD, Quaranta V, et al: Expression of lalike antigens in normal human nonlymphoid tissues. Transplantation 1981;31:75–78
Chiba M, Iizuka M, Masamune O: Ubiquitous expression of HLA-DR antigens on human small intestinal epithelium. Gastroenterol Jpn 1988;23:109–116
Iizuka M, Chiba M, Ohta H, et al: Expression of HLA-DR antigens on colonic epithelium in ulcerative colitis. Gastroenterol Jpn 1987;22:571–577
Selby WS, Janossy G, Mason DY, et al: Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease. Clin Exp Immunol 1983;53:614–618
Hirata I, Austin LL, Blackwell WH, et al: Immunoelectron microscopic localization of HLA-DR antigen in control small intestine and colon and in inflammatory bowel disease. Dig Dis Sci 1986;31:1317–1330
Fais S, Pallone F, Squarcia O, et al: HLA-DR antigens on colonic epithelial cells in inflammatory bowel disease: I. Relation to the state of activation of lamina propria lymphocytes and to the epithelial expression of other surface markers. Clin Exp Immunol 1987;68:605–612
McDonald GB, Jewell DP: Class II antigen (HLA-DR) expression by intestinal epithelial cells in inflammatory diseases of colon. J Clin Pathol 1987;40:312–317
Horie Y, Chiba M, Iizuka M, et al: Colonie lymphoid cell subsets and epithelial HLA-DR antigens in familial polyposis coli. Gastroenterol Jpn 1989;24(6) (in press)
Darr AS, Fuggle SV, Ting A, et al: Anomolous expression of HLA-DR antigens on human colorectal cancer cells. J Immunol 1982;129:447–449
Matsueda K, Umeda N, Shimojo E, et al: A trial of new homehyperalimentation for short bowel syndrome-its therapeutic application for inflammatory bowel disease. Inflammatory bowel disease. University of Tokyo Press, Tokyo. 1984;283–294
Iizuka M, Chiba M, Igarashi K, et al: Effects of elemental diet (ED) in Crohn’s disease Discrepancy between clinical and morphological improvements. JJSCP (J Jap Soc Coloproctology) 1989;42:327–333
Best WR, Becktel JM, Singleton JW, et al: Development of a Crohn’s disease activity index. Gastroenterology 1976;70:439–444
Chiba M, Ohta H, Nagasaki A, et al: Lymphoid cell subsets in normal human small intestine. Gastroenterol Jpn 1986;21:336–343
Wang CY, Al-Katib A, Lane CL, et al: Induction of HLA-DC/ DS (Leu 10) antigen expression by human precursor B cell lines. J Exp Med 1983;158:1757–1762
Matsumoto K: Studies of monoclonal antibodies specific to human leucocyte differentiation antigens. J Kurume Med Assoc 1984;47:1382–1397 (in Jpn)
Lampert IA, Suitters AJ, Chisholm PM: Expression of la antigen on epidermal keratinocytes in graft-versus-host disease. Nature 1981;293:149–150
Masson DW, Dallman M, Barclay AN: Graft-versus-host disease induces expression of la antigen in rat epidermal cells and gut epithelium. Nature 1981;293:150–151
Bottazzo GF, Dean BM, McNally JM, et al: In situ characterization of autoimmune phenomena and expression of HLA molecules in the pancreas in diabetes insulitis. N Engl J Med 1985;313:353–360
Foulis AK: Class II major histocompatibility complex and organ specific autoimmunity in man. J Pathol 1986;150:5–11
Bland PW, Warren LG: Antigen presentation by epithelial cells of the rat small intestine. I. Kinetics, antigen specificity and blocking by anti-Ia antisera. Immunology 1986;58:1–7
Bland PW, Warren LG: Antigen presentation by epithelial cells of the rat small intestine. II. Selective induction of suppressor T cells. Immunology 1986;58:9–14
Londei M, Botazzo GF, Feldmann M: Human T-cell clones from autoimmune thyroid glands: specific recognition of autologous thyroid cells. Science 1985;228:85–89
Janeway CA, Bottomly K, Babich J, et al: Quantitative variation in Ia antigen expression plays central role in immune regulation. Immunol Today 1984;5:99–105
Goodman MJ, Skinner JM, Truelove SC: Abnormalities in the apparently normal bowel mucosa in Crohn’s disease. Lancet 1976;l:275–278
Korelitz BI, Sommers SC: Rectal biopsy in patients with Crohn’s disease. Normal mucosa on sigmoidoscopic examination. JAMA 1977;237:2742–2744
Author information
Authors and Affiliations
Additional information
This work was partly supported by a grant from the Research Committee for Intractable Intestinal Disease, Ministry of Health and Welfare, Japan.
Rights and permissions
About this article
Cite this article
Chiba, M., Iizuka, M., Horie, Y. et al. HLA-DR antigen expression in macroscopically uninvolved areas of intestinal epithelia in Crohn’s disease. Gastroenterol Jpn 24, 365–372 (1989). https://doi.org/10.1007/BF02774341
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02774341