Abstract
Many molecular marker techniques are available today. PCR-based approaches are in demand because of their simplicity and requirement for only small quantities of sample DNA. Nonanchored inter simple sequence repeats (ISSRs) are arbitrary multiloci markers produced by PCR amplification with a microsatellite primer. They are advantageous because no prior genomic information is required for their use. We found the technique stable across a wide range of PCR parameters. Polymorphisms were abundant among 7 dicot species tested with 2 tri-nucleotide and 2 tetra-nucleotide primers. Thus, nonanchored ISSR markers are a good choice for DNA fingerprinting.
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Abbreviations
- AFLPs:
-
amplified fragment length polymorphisms
- CTAB:
-
cetyltrimethylammonium bromide
- ISSR:
-
inter simple sequence repeat
- MP-PCR:
-
microsatellite-primed PCR
- PCR:
-
polymerase chain reaction
- RAPD:
-
random amplified polymorphic DNA
- SSRs:
-
simple sequence repeats
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Bornet, B., Branchard, M. Nonanchored Inter Simple Sequence Repeat (ISSR) markers: Reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep 19, 209–215 (2001). https://doi.org/10.1007/BF02772892
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DOI: https://doi.org/10.1007/BF02772892