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Microarray analysis of adipose tissue gene expression profiles between two chicken breeds

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Abstract

The chicken is an important model organism that bridges the evolutionary gap between mammals and other vertebrates and provides a major protein source from meat and eggs throughout the world. Excessive accumulation of lipids in the adipose tissue is one of the main problems faced by the broiler industry nowadays. In order to visualize the mechanisms involved in the gene expression and regulation of lipid metabolism in adipose tissue, cDNA microarray containing 9 024 cDNA was used to construct gene expression profile and screen differentially expressed genes in adipose tissue between broilers and layers of 10 wk of age. Sixty-seven differentially expressed sequences were screened out, and 42 genes were found when blasted with the GenBank database. These genes are mainly related to lipid metabolism, energy metabolism, transcription and splicing factor, protein synthesis and degradation. The remained 25 sequences had no annotation available in the GenBank database. Furthermore, Northern blot and semi-quantitative RT-PCR were developed to confirm 4 differentially expressed genes screened by cDNA microarray, and it showed great consistency between the microarray data and Northern blot results or semi-quantitative RT-PCR results. The present study will be helpful for clarifying the molecular mechanism of obesity in chickens.

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Abbreviations

Apo-Al:

Apolipoprotein AI gene

ARP8:

actin-related protein 8 gene

GAPDH:

glyseraldehyde-3-phosphate dehy-drogenase gene

HDL:

high-density lipoprotein

LPL:

lipoprotein lipase gene

RT-PCR:

reverse transcription polymerase chain reaction

SNP:

single nucleotide polymorphism

TnI:

troponin I gene

VLDL:

very low density lipoprotein

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Authors and Affiliations

  1. College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, PR China

    Hongbao Wang, Hui Li, Qigui Wang, Yuxiang Wang, Huabin Han & Hui Shi

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  1. Hongbao Wang
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  2. Hui Li
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  3. Qigui Wang
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  4. Yuxiang Wang
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  5. Huabin Han
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  6. Hui Shi
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Correspondence to Hui Li.

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Wang, H., Li, H., Wang, Q. et al. Microarray analysis of adipose tissue gene expression profiles between two chicken breeds. J. Biosci. 31, 565–573 (2006). https://doi.org/10.1007/BF02708408

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