Abstract
The effects of hepatic stimulatory substance (HSS) on cirrhotic and noncirrhotic rats were studied after 70% partial hepatectomy. Liver cirrhosis was produced by weekly intragastric infusion of chloroform for 12–16 weeks. The HSS was prepared by extraction from the livers of weanling mice. Rats in the experimental group were injected with 5 ml HSS after 70% partial hepatectomy, and those in the control group received normal saline. The results showed that the3H-thymidine incorporation was higher in the HSS group 24 h after partial hepatectomy in both cirrhotic and non-cirrhotic rats, and persistently higher in the non-cirrhotic rats at 48h. Total DNA was significantly higher in the HSS group of non-cirrhotic rats 24 and 48 h after partial hepatectomy. The restituted liver volume and weight was significantly higher in non-cirrhotic rats 48 h after partial hepatectomy, while there was no significant difference between the HSS and the control groups in the cirrhotic rats. The HSS induced significant effects on3H-thymidine incorporation in the non-cirrhotic liver, resulting in increasing liver weight, volume and total DNA 48 h after partial hepatectomy. In cirrhotic rats, the3H-thymidine incorporation was higher in the HSS group at 24 h after partial hepatectomy, though not showing any increase at 48 h, but the regeneration of liver weight, volume and total DNA at 48 h showed no difference between the HSS group and the control group.
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References
Bucher NLR, Malt RA (1971) Regeneration of the liver and kidney. Little, Brown, Boston
Bucher NLR (1982) Thirty years of liver regeneration: a distillate. In: Sato GH, Pardee AB, Sirbasku DA (eds) Cold Spring Harb Symp Quant Biol 9: 15–24
Hoorn-Hickman RV, Kahn D, Green J, Macleod HA, Terblanche J (1981) Is there a regeneration stimulator substance in the effluent from perfused partially hepatectomized livers? Hepatology 1: 287–293
Fleig WE, Hoss G (1989) Partial purification of rat hepatic stimulator substance and characterization of its action on hepatoma cells and normal hepatocytes. Hepatology 9: 240–248
LaBrecque DR, Steele G, Fogerty S, Wilson M, Barton J (1987) Purification and physicalchemical characterization of hepatic stimulator substance. Hepatology 7: 100–106
Zarnegar R, Michalopoulos G (1989) Purification and biological characterization of human hepatopoietin A: a polypeptide growth factor for hepatocytes. Cancer Res 49: 3314–3320
Morley CGD, Kingdon HS (1973) The regulation of cell growth. Identification and partial characterization of a DNA synthesis stimulating factor from the serum of partially hepatectomized rats. Biochim Biophys Acta 308: 260–275
LaBrecque DR, Wilson M, Fogerty S (1984) Stimulation of HTC hepatoma cell growth in vitro by hepatic stimulator substance (HSS): interaction with serum, glucagon, epidermal growth factor and platelet-derived growth factor. Exp Cell Res 150: 419–429
LaBrecque DR, Pesch LA (1975) Preparation and partial characterization of hepatic regenerative stimulator substance from rat liver. J Physiol 248: 272–284
Starzl TE, Terblanche J, Porter KA, Jones AF, Usui S, Mazzoni G (1979) Growth-stimulating factor in canine liver. Lancet 1: 127–130
Terblanche J, Porter KA, Starzl TE, Moor J, Patzelt L, Hayashida N (1980) Stimulation of hepatic regeneration after 44% partial hepatectomy by infusion of a cytosol extract from dog liver. Surg Gynecol Obstet 151: 538–544
Fleig WE, Lehmann H, Wagner H, Hoss G, Ditschuneit H (1986) Hepatic regeneration stimulator substance in the rabbit: relation to liver regeneration after partial hepatectomy. J Hepatol 3: 19–26
Nagasue N, Yukaya H, Ogawa Y, Nakamura T (1987) Human liver regeneration after major hepatic resection: a study of normal liver and livers with chronic hepatitis and cirrhosis. Ann Surg 206: 30–39
Chen MF, Hwang TL, Hung CF (1991) Human liver regeneration after major hepatectomy—A study of liver volume by computerized tomography. Ann Surg 213: 227–229
Proctor E, Chatamra K (1982) High yield micronodular cirrhosis in the rat. Gastroenterology 83: 1183–1190
Chen MF, Hwang TL (1994) The regeneration of cirrhotic liver after partial hepatectomy: A study using the rat chloroform induced cirrhotic model. J Biochem Sci 18: 71–75
LaBrecque DR, Steele G, Fogerty S, Wilson M, Barton J (1987) Purification and physicalchemical characterization of hepatic stimulator substance. Hepatology 7: 110–116
Higgins M, Anderson RM (1931) Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12: 186–202
Hwang TL, Chen MF, Chen TJ (1993) Augmentation of liver regeneration with glucagon after partial hepatectomy in rats. J Formos Med Assoc 92: 725–728
Macintosh E, Gauthier T, Pettigrew N, Minuk G (1992) Liver regeneration and the effect of exogenous putrescine on regenerative activity after partial hepatectomy in cirrhotic rats. Hepatology 16: 1428–1433
Baskin G, Henderson GI, Schenker S (1988) Ethanol and hepatic regeneration. Hepatology 8: 408–411
Digernse V, Bronstad G, Sand TE, Christofferson T (1982) The proliferative response of rat liver parenchymal cells after partial hepatectomy: a methodological study comparing flow cytometry of nuclear DNA content in vivo and in vitro uptake of thymidine., Cell Tissue Kinet 15: 521–528
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Hwang, TL., Yu, HC., Chen, PC. et al. Liver regeneration following partial hepatectomy and stimulation by hepatic stimulatory substance in cirrhotic and non-cirrhotic rats. Res. Exp. Med. 195, 201–208 (1995). https://doi.org/10.1007/BF02576789
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DOI: https://doi.org/10.1007/BF02576789