Abstract
The relationship between HLA determinants on effector and target cells and cell-mediated cytotoxicity was studied using the release of 51Cr from measles virus-infected PHA-blasts. HLA compatibility between effector and target cells was not required if effector lymphocytes were derived from measles seropositive adults, from a patient with SSPE, and from children after live measles vaccination. Cytotoxicity was always abolished after removal of Fc receptor-bearing lymphoid cells. In these donors, the effect is, therefore, probably due to K cells. In contrast, lymphocytes from children with acute measles preferentially killed those virus-infected target cells with which they shared HLA antigens. Selective lytic activity was still observed after elimination of Fc receptor-bearing lymphoid cells. It is suggested that HLA-dependent killer cells represent specific cytotoxic T lymphocytes. These cells seem to be limited to the acute phase of measles.
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Abbreviations
- CTL:
-
cytotoxic T lymphocytes
- SSPE:
-
subacute sclerosing panencephalitis
- HI:
-
hemagglutination inhibition
- PBL:
-
peripheral blood lymphoid cells
- FCS:
-
fetal calf serum
- CRBC:
-
chicken red blood cells
- PHA:
-
phytohemagglutinin
- PBS:
-
phosphate-buffered saline
- BSS:
-
balanced salt solution
- ADCC:
-
antibody-dependent cellular cytotoxicity
- FcR:
-
Fc receptor-bearing lymphoid cells
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Supported by Grant Kr 376/8 from the Deutsche Forschungsgemeinschaft
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Kreth, H.W., Meulen, V.t. & Eckert, G. Demonstration of HLA restricted killer cells in patients with acute measles. Med Microbiol Immunol 165, 203–214 (1979). https://doi.org/10.1007/BF02152920
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DOI: https://doi.org/10.1007/BF02152920