Abstract
In order to examine the toxicity of silane, male ICR mice were exposed to silane for 30 min (n=8), 1 or 4 h (n=12) at concentrations of 2500, 5000, 7500 (30-min experiment only) or 10000 ppm. In the 1- and 4-h experiments, 12 mice were divided into two sub-groups: four for 2-day observation and eight for 2-week observation. The mortality was six deaths out of eight mice exposed to 10000 ppm for 4 h. No deaths occurred in any of the other experiments. In the mice sacrificed 2 days after the exposure, acute renal tubular necrosis was observed at 10000 ppm (1-h exposure) or at 2500 ppm or more (4-h exposure). Reduction in body weight, increase in relative kidney weight and blood urea nitrogen (BUN) level, and splenic atrophy and inflammatory changes of the nasal mucosa were also seen in the 10000 ppm-4 h exposure group. In the mice sacrificed 2 weeks after the exposure, tubulo-interstitial nephritis (TIN) developed at 7500 ppm or more (30-min exposure) or at 5000 ppm or more (1- and 4-h exposure). BUN increased in a dose-dependent manner, and BUN in TIN positive mice was significantly higher than that in TIN negative mice (1- and 4-h exposure). No histopathological changes were observed in the glomeruli. These results indicate that the LC50 of silane in mice is between 5000 and 10000 ppm for 4-h exposure and is greater than 10000 ppm for 1-h or 30-min exposure. The target site of silane toxicity is the renal tubule, and the no-observed-effect levels of silane for acute inhalation exposure in mice are 1000 ppm for 4-h exposure (from the previous report of our research group), 2500 ppm for 1-h exposure and 5000 ppm for 30-min exposure.
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References
American Conference of Governmental Industrial Hygienists (1985) Documentation of the threshold limit values and biological exposure indices, 5th edition. ACGIH, Cincinnati Ohio, p 528
Bohle A, Mackensen-Haen S, Gise HV (1987) Significance of tubulointerstitial changes in the renal cortex for the excretory function and concentration ability of the kidney: a morphometric contribution. Am JNephrol 7: 421–433
Brezis M, Rosen S, Epstein FH (1991) Acute renal failure. In: Brenner BM, Rector FC (ed) The kidney, 4th edition. WB Saunders Co., Philadelphia Pennsylvania, pp 993–1061
Eschbach JW, Adamson JW (1991) Hematologic consequences of renal failure. In: Brenner BM, Rector FC (ed) The kidney, 4th edition. WB Saunders Co., Philadelphia Pennsylvania, pp 2019–2035
Griffith RC (1990) Thymus gland. In: Kissane JM (ed) Anderson's pathology, 9th edition. CV Mosby Co., St Louis Missouri, pp 1493–1516
Kassirer JP (1971) Clinical evaluation of kidney function — glomerular function. N Engl J Med 285: 385–390
Kitchen DR (1989) Toxic gas health and safety controls for semiconductor manufacturing. In: American Conference of Governmental Industrial Hygienists. Hazard assessment and control technology in semiconductor manufacturing. Lewis Publishers Inc., Chelsea Michigan, pp 151–159
MacEven JD, Vernot EH (1972) Acute inhalation exposure of rats and mice to silane. In: Toxic Hazards Research Unit Annual Report 1972. US Department of Commerce, Springfield Virginia, pp 53–55
Muehrcke RC, Pirani CL (1968) Arsine-induced anuria. A correlative clinicopathological study with electron microscopic observations. Ann Int Med 68: 853–866
Muehrcke RC, Rosen S, Pirani CL, Kark RM (1964) Renal lesions in patients recovering from acute renal failure. J Lab Clin Med 64: 888
Omae K, Sakai T, Sakurai H, Yamazaki K, Shibata T, Mori K, Kudo M, Kanoh H, Tati M (1992) Acute and subacute inhalation toxicity of silane 1000 ppm in mice. Arch Toxicol 66: 750–753
Pasternack A, Tallqvist G, Kuhlback B (1970) Occurrence of interstitial nephritis in acute renal failure. Acta Med Scand 187: 27–31
World Health Organization (1991) Principles and methods for the assessment of nephrotoxicity associated with exposure to chemicals. WHO, Geneva, p 266
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Takebayashi, T. Acute inhalation toxicity of high concentrations of silane in male ICR mice. Arch Toxicol 67, 55–60 (1993). https://doi.org/10.1007/BF02072036
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DOI: https://doi.org/10.1007/BF02072036