Abstract
Acetol and methylglyoxal are intermediates of the intrahepatic metabolism of acetone leading to pyruvate formation. In hepatocytes prepared from fasted streptozotocin-induced diabetic mice, net glucose production could be measured from methylglyoxal but not from acetone or acetol. Insulin increased glucose formation from methylglyoxal in a concentration-dependent manner, whereas it was ineffective when pyruvate was used as substrate. Drug oxidation, as evidenced byp-aminophenol formation from aniline, was enhanced by methylglyoxal, and insulin proved to be stimulatory in this case as well. It is concluded that insulin might be involved in the regulation of glucose formation from methylglyoxal, but its mode of action is not yet clear.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Argiles, J. M., Trends Biochem. Sci.11 (1986) 61.
Casazza, J. P., Felver, M. E., and Veech, R. L., J. Biol. Chem.259 (1984) 231.
Owen, O. E., Trapp, V. E., Skutches, C. L., Mozzoli, M. A., Hoeldtke, R. D., Boden, G., and Reichard, G. A., Diabetes31 (1982) 242.
Reichard, G. A., Skutches, C. L., Hoeldtke, R. D., and Owen, O. E., Diabetes35 (1986) 668.
Hetényi, G., Byers, M., and Ferrarotto, C., Hormone Metabol. Res.19 (1987) 143.
Kosugi, K., Chandramouli, V., Kumaran, K., Schumann, W. C., and Landau, B. R., J. Biol. Chem.261 (1986) 13179.
Stöhr, R., Biochem. Zeitschr.240 (1936) 26.
Kermack, W. O., Lambil, C. G., and Slater, R. H., Biochem. J.21 (1927) 40.
Gonzalez, F. J., Pharmac. Rev.40 (1989) 243.
Thurman, R. G., and Kauffman, F. C., Pharmac. Rev.31 (1980) 229.
Grant, H. M., and Duthie, S. J., Biochem. Pharmacol.36 (1987) 3647.
Mandl, J., Garzó, T., Mészáros, K., and Antoni, F., Biochim. Biophys. Acta586 (1979) 560.
Jauregui, H. O., Hayner, N. T., Driscoll, J. L., Williams-Holland, R., Lipsky, M. H., and Galletti, P. M., In vitro17 (1980) 1100.
Bergmeyer, H. U., and Bernt, E., in: Methods in Enzymatic Analysis, p. 123. Ed. H. U. bergmeyer. Academic Press, New York 1963.
David, G. S., and Reisfeld, R. A., Biochemistry13 (1974) 1014.
Schenkman, J. B., Remmer, H., and Estabrook, R. W., Molec. Pharmac.3 (1967) 113.
Burton, K., Biochem. J.62 (1956) 315.
Racker, E., J. Biol. Chem.190 (1951) 685.
Kalapos, M. P., Garzó, T., Antoni, F. and Mandl, J., Biochim. Biophys. Acta1092 (1991) 284.
Kalapos, M. P., Mandl, J., Bánhegyi, G., Antoni, F., and Garzó, T., Int. J. Biochem.26 (1994) 1069.
Bánhegyi, G., Garzó, T., Antoni, F., and Mandl, J., Biochem. Pharmac.37 (1988) 4157.
Dicker, E., McHugh, T., and Cederbaum, A. I., Biochim. Biophys. Acta1035 (1990) 249.
Kalapos, M. P., Schaff, Z., Garzó, T., Antoni, F., and Mandl, J., Toxic. Letters58 (1991) 181.
Groen, A. K., van Roermund, C. W. T., Vervoorn, R. C., and Tager, J. M., Biochem. J.237 (1986) 379.
Kalapos, M. P., Riba, P., Garzó, T., Antoni, F., and Mandl, J., Int. J. Biochem.24 (1992) 211.
Hers, H. G., A. Rev. Biochem.45 (1976) 167.
Pilkis, S. J., El-Maghrabi, M. R., and Claus, T. H., A. Rev. Biochem.57 (1988) 755.
Granner, D., and Pilkis, S. J., J. Biol. Chem.265 (1990) 10173.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kalapos, M.P., Riba, P., Garzó, T. et al. Glucose formation from methylglyoxal in hepatocytes from streptozotocin-induced diabetic mice: the effect of insulin. Experientia 52, 827–830 (1996). https://doi.org/10.1007/BF01923997
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01923997