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A detrimental effect of platelets on mouse embryo development

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Abstract

We reported previously that serum prepared by delayed centrifugation of whole blood (DC serum), which is used widely in human in vitro fertilization and embryo transfer programs, has a detrimental effect on embryonic development. In an attempt to understand the mode of production of embryo-toxic factors in DC serum, we developed a model in which blood coagulation is initially blocked and later induced. The serum prepared in this fashion from whole blood (WB serum) is detrimental to mouse embryo development, as is DC serum. The toxicity of WB serum appears to derive mainly from the platelet release reaction during blood coagulation. Adenosine diphosphate and/or its degradation products may act directly to impair embryonic development and appear to be the main embryo-toxic substance(s) resulting from the platelet release reaction.

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Keio University School of Medicine, Tokyo, Japan

    Masao Jinno & Rihachi Iizuka

  2. Department of Obstetrics and Gynaecology, Ogikubo Hospital, Tokyo, Japan

    Etsurou Iida

Authors
  1. Masao Jinno
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  2. Etsurou Iida
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  3. Rihachi Iizuka
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Jinno, M., Iida, E. & Iizuka, R. A detrimental effect of platelets on mouse embryo development. J Assist Reprod Genet 4, 324–330 (1987). https://doi.org/10.1007/BF01555379

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  • DOI: https://doi.org/10.1007/BF01555379

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