Summary
Electron microscope cytochemical studies demonstrate that the plasma membrane of cultured aortic endothelial cells contain significant amounts of adenosine diphosphatase. The activity is due to an ectoenzyme on the upper surface of the cell. Intracellular activity was noted in multilamellar bodies.
Similar content being viewed by others
References
Born, G. V. R. (1965) Platelets in thrombogenesis: mechanism and inhibition of platelet aggregation.Ann. R. Coll. Surg. 36, 200–6.
Cooper, D. R., Lewis, G. P., Liebbrman, G. E., Webb, H. &Westwick, J. (1979) ADP metabolism in vascular tissue, a possible thrombo-regulatory mechanism.Thromb. Res. 14, 901–14.
Crutchley, D. J., Eling, T. E. &Anderson, M. W. (1978) ADPase activity of isolated perfused rat lung.Life Sci. 22, 1413–20.
Dosne, A. M., Legrand, C., Bauvois, B., Badevin, E. &Caen, J. P. (1978) Comparative degradation of adenyl nucleotides by cultured endothelial cells and fibroblasts.Biochem. biophys. Res. Commun. 85, 183–9.
Gan, J. E. T. &Firkin, B. G. (1978) Adenesine diphosphate (ADP) breakdown in human plasma with reference to platelet aggregation and uraemia.Thromb. Diath. Haemorrh. 19, 437–50.
Glasgow, J. G., Schade, R. &Pitlick, F. A. (1978) Evidence that ADP hydrolysis by human cells is related to thrombogenic potential.Thromb. Res. 13, 255–66.
Heyns, A. Du P., Van Den Berg, D. J., Potgieter, G. M. &Retief, F. P. (1974) The inhibition of platelet aggregation by an aorta intima extract.Thromb. Diath. Haemorrh. 32, 417–31.
Ireland, D. M. & Mills, D. C. B. (1964) Degradation of adenosine diphosphate in plasma.Biochem. J. 92, 30P.
Lieberman, G. E., Lewis, G. P. &Peters, T. J. (1977) A membrane bound enzyme in rabbit aorta capable of inhibiting adenosine diphosphate-induced platelet aggregation.Lancet II, 330–2.
Marchesi, V. T. &Barnett, R. J. (1963) The demonstration of enzymatic activity in pinocytic vesicles of blood capillaries with the electron microscope.J. Cell Biol. 17, 547–56.
Smith, G. P. &Peters, T. J. (1981) Subcellular localization and properties of adenosine diphosphatase activity in human polymorphonuclear leukocytes.biochim. biophys. Acta 673, 234–42.
Smith, G., Smith, G. D. &Peters, T. J. (1980) A direct rapid, radioassay for adenosine diphosphatase.Clin. Chim. Acta 201, 287–91.
Tschopp, T. B. &Baumgartner, H. R. (1976) Enzymatic removal of ADP from plasma: unaltered platelet adhesion but reduced aggregation on subendothelium and collagen fibrils.Thromb. Haemostas. 35, 334–41.
Wilson, P. D., Rustin, G. J. S., Smith, G. P. &Peters, T. J. (1981) Electron microscopic cytochemical localization of nucleoside phosphatases in normal and chronic granulocytic leukaemic human neutrophils.Histochem. J. 13, 73–84.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wilson, P.D., Lieberman, G.E. & Peters, T.J. Ultrastructural localization of adenosine diphosphatase activity in cultured aortic endothelial cells. Histochem J 14, 215–219 (1982). https://doi.org/10.1007/BF01041216
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01041216