Skip to main content
SpringerLink
Log in

Inflammation-induced synthesis of proteoheparan sulfate: A novel acute-phase reactant in rat hepatocytes

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

The synthesis of proteoheparan sulfate in hepatocytes is positively regulated under acute-phase conditions produced either by turpentine or deep back incision. In both cases the incorporation of [35S]sulfate and [14C]glucosamine is doubled during a 4-h incubation period if compared with control rat hepatocytes. Neither the fractional secretion rate of heparan sulfate into the medium (< 0.1 of cell-associated glycosaminoglycans) nor the composition of newly formed proteoglycans in hepatocytes are affected during acute phase reaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ninomiya, Y., R.-I. Hata, andY. Nagai. 1981. Active synthesis of glycosaminoglycans by liver parenchymal cells in primary culture.Biochim. Biophys. Acta 675:248–255.

    Google Scholar 

  2. Oldberg, A., andM. Höök. 1977. Structure and metabolism of rat liver heparan sulfate.Biochem. J. 164:75–81.

    Google Scholar 

  3. Prinz, R., U. Klein, P. R. Sudhakaran, W. Sinn, K. Ullrich, andK. v. Figura. 1980. Metabolism of sulfated glycosaminoglycans in rat hepatocytes. Synthesis of heparan sulfate and distribution into cellular and extracellular pools.Biochim. Biophys. Acta 630:402–413.

    Google Scholar 

  4. Oldberg, A., L. Kjellen, andM. Höök. 1979. Cell-surface heparan sulfate. Isolation and characterization of a proteoglycan from rat liver membranes.J. Biol. Chem. 254:8505–8510.

    Google Scholar 

  5. Kjellen, L., A. Oldberg, andM. Höök. 1980. Cell-surface heparan sulfate. Mechanisms of proteoglycan-cell association.J. Biol. Chem. 255:10407–10413.

    Google Scholar 

  6. Kjellen, L., I. Pettersson, andM. Höök. 1981. Cell-surface heparan sulfate: An intercalated membrane proteoglycan.Proc. Natl. Acad. Sci. U.S.A. 78:5371–5375.

    Google Scholar 

  7. Riesenfeld, J., M. Höök, andU. Lindahl. 1982. Biosynthesis of heparan sulfate in rat liver.J. Biol. Chem. 257:7050–7055.

    Google Scholar 

  8. Gressner, A. M. 1983. Hepatic proteoglycans—a brief survey of their pathobiochemical implications.Hepatogastroenterology 30:225–229.

    Google Scholar 

  9. Beisel, W. R. 1980. Effects of infection on nutritional status and immunity.Fed. Proc. 39:3105–3108.

    Google Scholar 

  10. Koj, A. 1974. Acute-phase reactants: Their synthesis, turnover, biological significance.In Structure and Function of Plasma Proteins, Vol. 1. A. C. Allison, editor. Plenum Press, London. 75–125.

    Google Scholar 

  11. Kushner, I. 1982. The phenomenon of the acute phase response.Ann. N. Y. Acad. Sci.,389:39–48.

    Google Scholar 

  12. Schreiber, G., andG. Howlett. 1983.In Plasma Protein Secretion by the Liver.H. Glaumann et al., editors. Academic Press, London. 423–449.

    Google Scholar 

  13. Gressner, A. M., H. Pazen, andH. Greiling. 1977. The biosynthesis of glycosaminoglycans in normal rat liver and in response to experimental hepatic injury.Z. Physiol. Chem. 358:825–833.

    Google Scholar 

  14. Gressner, A. M., S. Heinrigs, andP. Grouls. 1982. The sequence of changes in the biosynthesis of sulfated glycosaminoglycans in acute, experimental liver disease.J. Clin. Chem. Clin. Biochem. 20:15–24.

    Google Scholar 

  15. Gressner, A. M., andA. Vasel, 1985. Developmental changes of proteoglycan synthesis in rat liver and isolated hepatocytes.Mech. Age. Dev. 31:307–327.

    Google Scholar 

  16. Seglen, P. O. 1976. Preparation of isolated rat liver cells.Methods Cell Biol.,13:29–83.

    Google Scholar 

  17. Labarca, C., andK. Paigen. 1980. A simple rapid, and sensitive DNA assay procedure.Anal. Biochem. 102:344–352.

    Google Scholar 

  18. Clauss, A. 1957. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens.Acta Hämatol. 17:237–246.

    Google Scholar 

  19. Jamieson, J. C., H. A. Kaplan, B. M. R. N. J. Woloski, M. Hellmann, andK. Ham, 1982. Glycoprotein biosynthesis during the acute-phase response to inflammation.Can. J. Biochem. Cell. Biol. 61:1041–1048.

    Google Scholar 

  20. Höök, M., L. Kjellen, S. Johansson, andJ. Robinson. 1984. Cell-surface glycosaminoglycans.Annu. Rev. Biochem. 53:847–869.

    Google Scholar 

  21. Northemann, W., T.Andus, V.Gross, M.Nagashima, G.Schreiber, and P. C.Heinrich. Messenger RNA activities of four acute-phase proteins during inflammation.FEBS Lett. 161:319–322.

  22. Baumann, H., W. A. Held, andF. G. Berger. 1984. The acute-phase response of mouse liver.J. Biol. Chem. 259:566–573.

    Google Scholar 

  23. Bauer, J., A. Kurdowska, T.-A. Tran-Thi, W. Budek, A. Koj, K. Decker, andP. C. Heinrich. 1985. Biosynthesis and secretion of α1 acute-phase globulin in primary cultures of rat hepatocytes.Eur. J. Biochem. 146:347–352.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Clinical Chemistry and Central Laboratory, University of Marburg Baldingerstrasse, D-3550, Marburg/Lahn, Germany

    Assadollah Djovkar & Axel M. Gressner

Authors
  1. Assadollah Djovkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Axel M. Gressner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Djovkar, A., Gressner, A.M. Inflammation-induced synthesis of proteoheparan sulfate: A novel acute-phase reactant in rat hepatocytes. Inflammation 11, 39–46 (1987). https://doi.org/10.1007/BF00917770

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00917770

Keywords

Search

Navigation