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Gastric carcinoma with lymphoid stroma

Analysis using mucin histochemistry and immunohistochemistry

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Summary

A total of 626 surgically resected gastric carcinomas were reviewed, and 24 cases (3.8%) of “gastric carcinoma with lymphoid stroma” were identified. The tumour cells were consistently arranged in an anastomosing trabecular or alveolar pattern and were densely infiltrated by lymphoid cells. The specimens were studied using mucin histochemistry and the indirect immunoperoxidase method to determine the histochemical properties of this form of gastric carcinoma. The tumour cells were consistently positive for concanavalin A paradoxical staining, class III and almost devoid of acidic mucins, features demonstrating preferential differentiation toward pyloric glands or pseudopyloric glands. Immunohistochemically, positive reactions for Leu M1 and lysozyme, marker substances of (pseudo)pyloric gland cells, were often observed. Carcinoembryonic antigen was positive in focal areas without (pseudo)pyloric glandular patterns. Secretory component was focally positive. HLA-DR was strongly expressed in most cancer cells and 17 tumours (71%) showed positivity for interleukin 1 (IL-1). The lymphoid stroma contained a high percentage of UCHL1-reactive T cells both within and around the cancer cell nests, while SL26-reactive B cells clustered in lymphoid follicles. A considerable number of T-lymphoid cells were also reactive for IL-1. A number of plasma cells with a predominance of IgG-type were distributed around the cancer cell nests. S-100 protein-positive dendritic cells were not identified. We speculate that the prominent lymphoid stroma including intraepithelial lymphocyte-like T cells with IL-1 receptors is possibly induced by IL-1 related mediators released from the HLA-DR-positive gastric cancer cells of the (pseudo)pyloric gland-type.

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References

  • Bland PW, Warren LG (1986) Antigen presentation by epithelial cells of the rat small intestine. II. Selective induction of suppressor T cells. Immunology 58:9–14

    Google Scholar 

  • Cerf-Bensussan N, Schneeberger EE, Bhan AK (1983) Immunohistologic and immunoelectron microscopic characterization of the mucosal lymphocytes of human small intestine by the use of monoclonal antibodies. J Immunol 130:2615–2622

    Google Scholar 

  • Cerf-Bensussan N, Quaroni A, Kurnick JT, Bhan AK (1984) Intraepithelial lymphocytes modulate Ia expression by intestinal epithelial cells. J Immunol 132:2244–2252

    Google Scholar 

  • Doughlas AP, Weetman AP (1975) Lymphocyte and the gut. Digestion 13:344–371

    Google Scholar 

  • Dower SK, Urdal DL (1987) The interleukin-1 receptor. Immunol Today 8:46–51

    Google Scholar 

  • Duijivestijn AM, Horst E, Pals ST, Rouse BN, Steere AC, Picker LJ, Meijer CJLM, Butcher EC (1988) High endothelial differentiation in human lymphoid and inflammatory tissue defined by monoclonal antibody HECA-452. Am J Pathol 130:147–155

    Google Scholar 

  • Fukamachi H (1986) Disorganization of stroma alters epithelial differentiation of the glandular stomach in adult mice. Cell Tiss Res 243:65–68

    Google Scholar 

  • Hamazaki M, Sawayama K, Kuriya T (1968) “Medullary carcinoma with lymphoid infiltration” of stomach. J Karyopathol 12:115–120 (in Japanese)

    Google Scholar 

  • Heidl G, Davaris P, Zwadlo G, Jagoda MS, Duchting S, Bierhoff E, Gruter T, Krieg V, Sorg C (1987) Association of macrophages detected with monoclonal antibody 25F9 with progression and pathobiological classification of gastric carcinoma. J Cancer Res Clin Oncol 113:567–572

    Google Scholar 

  • Hirata I, Berrebi G, Austin LL, Keren DF, Dobbins WO III (1986) Immunohistological characterization of intraepithelial and lamina propria lymphocytes in control ileum and colon and in inflammatory bowel disease. Digest Dis Sci 31:593–603

    Google Scholar 

  • Isaacson P (1982) Immunoperoxidase study of the secretory immunoglobulin system and lysozyme in normal and diseased gastric mucosa. Gut 23:578–588

    Google Scholar 

  • Katsuyama T, Ono K, Nakayama J, Kanai M (1985) Recent advances in mucosubstance histochemistry. In: Kawai K (ed) Gastric Mucus and Mucus Secreting cells. Excerpta Medica, Tokyo, pp 3–18

    Google Scholar 

  • Kurtin PJ, Pinkus GS (1985) Leukocyte common antigen. A diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies. Correlation with immunologic studies and ultrastructural localization. Human Pathol 16:353–365

    Google Scholar 

  • Kyogoku M, Sawai T, Mori S (1987) Defensive tissue response in human. Reactions before inflammation and “interstitial cells”. Nihon Rinsho 45:939–953 (in Japanese)

    Google Scholar 

  • MacCarty WC, Mahle AE (1921) Relation of differentiation and lymphocytic infiltration to postoperative longevity in gastric carcinoma. J Lab Clin Med 6:473–480

    Google Scholar 

  • McDonald GB, Jewell DP (1987) Class II antigen (HLA-DR) expression by intestinal epithelial cells in inflammatory diseases of colon. J Clin Pathol 40:312–317

    Google Scholar 

  • Moore OS Jr, Foote FW Jr (1949) The relativity favorable prognosis of medullary carcinoma of the breast. Cancer 2:635–642

    Google Scholar 

  • Nagura H, Sumi Y (1987) Immunocytochemical studies of the vascular endothelial cells in human Peyer's patches and solitary lymphoid follicles. In: Mesdecky J, McGhee JR, Bienenstock J, Ogra PL (eds) Recent Advances in Mucosal Immunity. Plenum Publishers, New York, pp 279–286

    Google Scholar 

  • Nakamura S, Sumi Y, Nagura H (1988) Ontogenic development of gut-associated lymphoid tissue of the rat: An immunohistochemical study. Acta Pathol Jpn 38:1267–1283

    Google Scholar 

  • Norton AJ, Ramsay AD, Smith SH, Beverely PCL, Isaacson PG (1986) Monoclonal antibody (UCHL1) that recognizes normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol 39:399–405

    Google Scholar 

  • Okamura T, Kodama Y, Kamegawa T, Sano C, Kumashiro R, Inokuchi K (1983) Gastric carcinoma with lymphoid stroma: Correlation to reactive hyperplasia in regional lymph nodes and prognosis. Jpn J Surg 13:177–183

    Google Scholar 

  • Okon E, Felder B, Epstein A, Marder R, Lukes R, Tyler C (1985) Monoclonal antibodies reactive with B lymphocytes and histiocytes in paraffin sections. Cancer 56:95–104

    Google Scholar 

  • Schultz RM (1987) Interleukin-1 and interferon-α: Cytokines that provide reciprocal regulation of macrophage and T cell function. Toxicol Pathol 15:333–337

    Google Scholar 

  • Sheibani K, Battifora H, Burke JS, Rappaport H (1986) Leu M1 antigen in human neoplasms. An immunohistologic study of 400 cases. Am J Surg Pathol 10:227–236

    Google Scholar 

  • Spencer J, Pugh S, Isaacson PG (1986) HLA-D region antigen expression on stomach epithelium in absence of autoantibodies. Lancet 8513:983

    Google Scholar 

  • Steiner PE, Maimon SN, Palmer WL, Kirsner JB (1948) Gastric cancer: Morphologic factors in five-year survival after gastrectomy. Am J Pathol 24:947–969

    Google Scholar 

  • Takami T, Ishii Y, Yuasa H, Kikuchi K (1985) Three distinct antigen systems on human B cell subpopulations as defined by monoclonal antibodies. J Immunol 134:828–834

    Google Scholar 

  • Tamaoki N (1985) Pathology of human neoplasms heterotransplantable to nude mice. An experimental approach to pathology of cancer-bearing individuals. Tr Soc Pathol Jpn 93:93–125 (in Japanese)

    Google Scholar 

  • Tsujitani S, Furukawa T, Tamada R, Okamura T, Yasumoto K, Sugimachi K (1987) Langerhans cells and prognosis in patients with gastric carcinoma. Cancer 59:501–505

    Google Scholar 

  • Tsutsumi Y (1986) Pathophysiological significance of intestinal metaplasia of the gastric mucosa: An immunohistochemical approach. In: Kasuya Y, Tsuchiya M, Nagao F, Matsuo Y (eds) Gastrointestinal Function. Regulation and Disturbances, vol 4, Excerpta Medica, Tokyo, pp 15–37

    Google Scholar 

  • Tsutsumi Y (1988) Differentiation of gastric cancer cells: Analysis using immunohistochemistry and mucin histochemistry. In: Watanabe S, Wolff M, Sommers SC (eds) Digestive Disease Pathology, vol 1. Field and Rich Inc, New York, pp 1–49

    Google Scholar 

  • Tsutsumi Y, Nagura H, Watanabe K (1984a) Immunohistochemical observations of carcinoembryonic antigen (CEA) and CEA-related substances in normal and neoplastic pancreas. Pitfalls and caveats in CEA immunohistochemistry. Am J Clin Pathol 82:535–542

    Google Scholar 

  • Tsutsumi Y, Nagura H, Watanabe K (1984b) Immune aspects of intestinal metaplasia of the stomach:An immunohistochemical study. Virchows Arch [Pathol Anat] 403:345–359

    Google Scholar 

  • van den Hooff A (1984) The part played by the stroma in carcinogenesis. Perspect Biol Med 27:498–509

    Google Scholar 

  • Watanabe H, Enjoji M, Imai T (1976) Gastric carcinoma with lymphoid stroma. Its morphologic characteristics and prognostic correlations. Cancer 38:232–243

    Google Scholar 

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  1. Department of Pathology, Tokai University School of Medicine, 259-11, Bohseidai, Isehara, Japan

    Nirush Lertprasertsuke & Yutaka Tsutsumi

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  1. Nirush Lertprasertsuke
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  2. Yutaka Tsutsumi
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Lertprasertsuke, N., Tsutsumi, Y. Gastric carcinoma with lymphoid stroma. Vichows Archiv A Pathol Anat 414, 231–241 (1989). https://doi.org/10.1007/BF00822027

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  • DOI: https://doi.org/10.1007/BF00822027

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