Dopaminergic binding sites in rat striatal slices and the action of guanyl nucleotides

Summary

Dopaminergic binding sites were studied in slices from rat striatum incubated in a physiological medium and using the two highly selective ligands ³H-apomorphine and ³H-domperidone. The clearly biphasic or stretched inhibition of the specific binding of these two ligands by domperidone or apomorphine, respectively allowed to define three distinct classes of binding site. It was demonstrated, by comparing the binding of the ³H-ligand added at the beginning of slice incubation or just before homogenisation of tissue and filtration, that the “specific” bindings only occurred during the incubation of slices. The inhibition constants (K _i values) of dopaminergic agents for the three classes of binding site as also the dissociation constants (K _d values) of ³H-ligands and the maximal capacity (B _max) of the three classes of binding site were closely similar to those of binding sites previously demonstrated on rat striatal membranes, namely D-2, D-3 and D-4 sites (Sokoloff et al. 1980a, b). Their identification on a preparation in which the cellular organisation is largely preserved rules out the possibility that these sites represent an artifact due to membrane preparation.

Unexpectedly the addition of guanyl nucleotides like GTP or GppNHp to the slice preparation decreased the binding of ³H-apomorphine to the high affinity sites (particularly to the D-2 sites) while D-4 site binding was correspondingly increased. The guanylnucleotide effect apparently took place before cell disruption and occurred at concentrations similar to those required in striatal membrane preparations. These observations, together with those indicating the presence of high affinity binding sites for dopaminergic agonists in intact striatal cells, suggest that a putative nucleotide regulatory unit of dopamine receptors, is not fully occupied by intracellular GTP but could be interacted with from the external face of the cell membrane.