Abstract
Extensive extraction studies with Triton X-100 revealed only LDH-1 (B4) but no trace of LDH-5 (A4) in one-cell and two-cell mouse and rat embryos. The LDH isozyme pattern of preimplantation mouse embryos changes from the maternally inherited B subunit isozyme (LDH-1) to a pattern dominated by LDH-5 when mouse blastocysts are cultured under conditions that prevent hatching but allow trophoblast giant cell transformation. During differentiation of mouse blastocysts in vitro, implantation is therefore not essential for the appearance of the A subunit form of LDH (LDH-5) coded for by the embryonic genome. Mechanisms controlling the expression of LDH-5 in mouse blastocysts during in vivo development are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Auerbach, S., and Brinster, R. L. (1967). Lactate dehydrogenase isozymes in the early mouse embryo. Exp. Cell Res. 4689.
Auerbach, S., and Brinster, R. L. (1968). Lactate dehydrogenase isozymes in mouse blastocyst cultures. Exp. Cell Res. 53313.
Barlow, P. W., and Sherman, M. I. (1972). The biochemistry of differentiation of mouse trophoblast: Studies on polyploidy. J. Embryol. Exp. Morphol. 27447.
Besley, G. T. N. (1976). Effect of Triton X-100 on the isoelectric focusing profile of fibroblast sphingomyelinase. FEBS Lett. 72101.
Brinster, R. L. (1971). The lactate dehydrogenase in the preimplantation embryos of Quackenbush and Swiss mice. FEBS Lett. 1741.
Engel, W., and Petzoldt, U. (1973). Early developmental changes of the lactate dehydrogenase isoenzyme pattern in mouse, rat, guinea-pig, Syrian hamster and rabbit. Humangenetik 20125.
Epstein, C. J. (1975). Gene expression and macromolecular synthesis during preimplantation embryonic development. Biol. Reprod. 1282.
Epstein, C. J., Kwok, L., and Smith, S. (1971). The source of lactate dehydrogenase in preimplantation mouse embryos. FEBS Lett. 1345.
Erickson, R. P., Spielmann, H., Mangia, F., Tennenbaum, D., and Epstein, C. J. (1975). Studies on lactate dehydrogenase isozymes in gametes and early development of mice. In Markert, C. L. (ed.), Isozymes, Vol. III, Academic Press, New York, pp. 313–324.
Fries, E. (1976). Determination of Triton X-100 binding to membrane proteins by polyacrylamide gel electrophoresis. Biochim. Biophys. Acta 455928.
Mangia, F., Erickson, R. P., and Epstein, C. J. (1976). Synthesis of LDH-1 during mammalian oogenesis and early development. Dev. Biol. 54146.
McLaren, A., and Hensleigh, H. C. (1975). Culture of mammalian embryos over the implantation period. In Balls, M., and Wild, A. E. (eds.), The Early Development of Mammals, Cambridge University Press, Cambridge, pp. 45–60.
Monk, M., and Ansell, J. (1976). Patterns of lactic dehydrogenase isozymes in mouse embryos over the implantation period in vivo and in vitro. J. Embryol. Exp. Morphol. 36653.
Monk, M., and Petzoldt, U. (1977). Control of inner cell mass development in cultured mouse blastocysts. Nature 265338.
Peacock, T. A. R., Schultz, G. A., and Browder, L. W. (1976). Lactate dehydrogenase in one-cell rabbit embryos extracted with Triton X-100. Biochem. Genet. 14523.
Pienkowski, M., Solter, D., and Koprowski, H. (1974). Early mouse embryos: Growth and differentiation in vitro. Exp. Cell Res. 85424.
Poznakhirkina, N. A., Serov, O. L., and Korochkin, L. I. (1975). A study on lactate dehydrogenase isozymes in rat ova. Biochem. Genet. 1365.
Rapola, J., and Koskimies, O. (1967). Embryonic enzyme patterns: Characterization of the single lactate dehydrogenase isozyme in preimplanted mouse ova. Science 1571311.
Sherman, M. I. (1972a). Biochemistry of differentiation of mouse trophoblast: Esterase. Exp. Cell Res. 75449.
Sherman, M. I. (1972b). The biochemistry of differentiation of mouse trophoblast: Alkaline phosphatase. Dev. Biol. 27337.
Sherman, M. I. (1975). Long term culture of cells derived from mouse blastocysts. Differentiation 351.
Spielmann, H., Erickson, R. P., and Epstein, C. J. (1973). The separation of lactate dehydrogenase X from other lactate dehydrogenase isozymes of mouse testes by affinity chromatography. FEBS Lett. 3519.
Spielmann, H., Erickson, R. P., and Epstein, C. J. (1974a). Immunochemical studies of lactate dehydrogenase and glucose-6-phosphate dehydrogenase in preimplantation mouse embryos. J. Reprod. Fertil. 40367.
Spielmann, H., Erickson, R. P., and Epstein, C. J. (1974b). The production of antibodies against mammalian LDH-1. Anal. Biochem. 59462.
Spielmann, H., Eibs, H.-G., Mentzel, C., and Nagel, D. (1977). Studies on the binding of antibody against mouse lactate dehydrogenase (isoenzyme X) by preimplantation mouse embryos. J. Reprod. Fertil. 5047.
Spindle, A. I., and Pedersen, R. A. (1973). Hatching, attachment, and outgrowth of mouse blastocysts in vitro: Fixed nitrogen requirements. J. Exp. Zool. 186305.
Whitten, W. K. (1971). Nutrient requirements for the culture of preimplantation embryos in vitro. In Raspé, G. (ed.), Advances in the Biosciences, Vol. 6, Pergamon Press, New York, pp. 129–139.
Author information
Authors and Affiliations
Additional information
This work was supported by grants of the Deutsche Forschungsgemeinschaft awarded to the Sonderforschungsbereich 29—“Embryonale Entwicklung and Differenzierung.”
Rights and permissions
About this article
Cite this article
Spielmann, H., Eibs, HG., Jacob-Müller, U. et al. Expression of lactate dehydrogenase isozyme 5 (LDH-5) in cultured mouse blastocysts in the absence of implantation and outgrowth. Biochem Genet 16, 191–202 (1978). https://doi.org/10.1007/BF00484077
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00484077