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Genotyping chickens for the B-G subregion of the major histocompatibility complex using restriction fragment length polymorphisms

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Abstract

Chicken B-G-subregion cDNA probes were used to analyze restriction fragment length polymorphisms (RFLP) of the B-G subregion of the chicken major histocompatibility complex. Genomic DNA from chickens representing 17 of the 27 standard B haplotypes were digested with restriction endonucleases and analyzed in Southern hybridizations with two cDNA clones from the B-G subregion. Each B-G genotype was found to produce a unique pattern of restriction fragments in these Southern hybridizations. With 15 of the 17 genotypes examined, the different genotypes could be readily distinguished in hybridizations produced with DNA digested with a single restriction enzyme, PVU II. The two additional genotypes produced nearly identical patterns in PVU II preparations and with three additional enzymes as well, but were readily distinguishable in Eco RI digestions. For many of the haplotypes, samples from several individuals in different flocks were examined. In every instance, genotyping by RFLP pattern was found to confirm the B-G allele assigned serologically.

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References

  • Abplanalp, H.: Inbred lines as genetic resources of chickens. In G. E. Dickerson and R. K. Johnson (eds.): Proceedings of the Third World Congress of Genetics Applied to Livestock Production. Volume 10, pp. 257–268, Lincoln, Nebraska, 1986

    Google Scholar 

  • Briles, W. E. and Briles, R. W.: Identification of haplotypes of the chicken major histocompatibility complex (B). Immunogenetics 15: 449–459, 1982

    Google Scholar 

  • Briles, W. E., McGibbon, W. H., and Irwin, M. R.: On multiple alleles effecting cellular antigens in the chicken. Genetics 35: 633–652, 1950

    Google Scholar 

  • Briles, W. E., Bumstead, N., Ewert, D. L., Gilmour, D. G., Gogusev, J., Hála, K., Koch, C., Longenecker, B. M., Nordskog, A. W., Pink, J. R. L., Schierman, L. W., Simonsen, M., Toivanen, A., Toivanen, P., Vaino, O., and Wick, G.: Nomenclature for chicken major histocompatibility (B) complex. Immunogenetics 15: 441–447, 1982

    Google Scholar 

  • Feinberg, A. P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983

    Google Scholar 

  • Goto, R., Miyada, C. G., Young, S., Wallace, R. B., Abplanalp, H., Bloom, S. E., Briles, W. E., and Miller, M. M.: Isolation of a cDNA clone from the B-G subregion of the chicken histocompatibility (B) complex. Immunogenetics 27: 102–109, 1988

    Google Scholar 

  • Hála, K.: Inbred lines of avian species. In A. Toivanen and P. Toivanen (eds.): Avian Immunology: Basis and Practice, Volume II, pp. 85–99, 1987

  • Koch, C., Skjedt, K., Toivanen, A., Toivanen, P.: New recombinants within the MHC (B-complex) of the chicken. Tissue Antigens 21: 129–137, 1983

    Google Scholar 

  • Lacy, E., Roberts, S., Evans, E. P., Burtenshaw, M. D., and Constantini, F. D.: A foreign β-globin gene in transgenic mice: integration at abnormal chromosomal positions and expression in inappropriate tissues. Cell 34: 343–358, 1983

    Google Scholar 

  • Longenecker, B. M. and Mosmann, T. R.: Restricted expression of an MHC alloamigen in cells of the erythroid series: a specific marker for erythroid differentiation. J Supramol Struct 13: 395–400, 1980

    Google Scholar 

  • Longenecker, B. M. and Mosmann, T. R.: Nomenclature for chicken MHC (B) antigens defined by monoclonal antibodies. Immunogenetics 13: 25–28, 1981

    Google Scholar 

  • Longenecker, B. M., Mosmann, T. R., and Shiozawa, C.: A strong, preferential response of mice to polymorphic antigenic determinants of the chicken MHC, analyzed with mouse hybridoma (monoclonal) antibodies. Immunogenetics 9: 137–147, 1979

    Google Scholar 

  • Miller, M. M., Goto, R., and Abplanalp, H.: Analysis of the B-G antigens of the chicken MHC by two-dimensional gel electrophoresis. Immunogenetics 20: 373–385, 1984

    Google Scholar 

  • Moon, R. T., Ngai, J., Wold, B. J., and Lazarides, E.: Tissue-specific expression of distinct spectrin and ankyrin transcripts in erythroid and nonerythroid cells. J Cell Biol 100: 152–160, 1985

    Google Scholar 

  • Mosmann, T. R., Gallatin, M., and Longenecker, B. M.: Alteration of apparent specificity of monoclonal (hybridoma) antibodies recognizing polymorphic histocompatibility and blood group determinants. J Immunol 125: 1152–1156, 1980

    Google Scholar 

  • Pink, J. R. L., Droege, W., Hála, K., Miggiano, V. C., and Ziegler, A.: A three-locus model for the chicken major histocompatibility complex. Immunogenetics 5: 203–216, 1977

    Google Scholar 

  • Salomonsen, J., Skjødt, K., Crone, M., and Simonsen, M.: The chicken erythrocyte-specific MHC antigen. Characterization and purification of the B-G antigen by monoclonal antibodies. Immunogenetics 25: 373–382, 1987

    Google Scholar 

  • Schierman, L. W. and Nordskog, A. W.: Relationship of blood type to histocompatibility in chickens. Science 134: 1008–1009, 1961

    Google Scholar 

  • Shen, P. F., Smith, E. J., and Bacon, L. D.: The ontogeny of blood cells, complement, and immunoglobulins in 3- to 12-week-old 15I5-B congenic white leghorn chickens. Poult Sci 63: 1083–1093, 1984

    Google Scholar 

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Miller, M.M., Abplanalp, H. & Goto, R. Genotyping chickens for the B-G subregion of the major histocompatibility complex using restriction fragment length polymorphisms. Immunogenetics 28, 374–379 (1988). https://doi.org/10.1007/BF00364237

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  • DOI: https://doi.org/10.1007/BF00364237

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