Abstract
A bacteriophage active against a marine Aeromonas sp. was isolated from surface sediments of the North Pacific Ocean at 825 m depth. The sensitive Aeromonas sp. grew between 0° and 33°C but plaques were formed only between 0° and 23°C with a maximum zone of lysis at 5° to 12°C. The phage was rapidly inactivated at temperatures above 45°C. The characteristic plaque formation occurred only on media made with sea-water but some lysis was obtained in distilled water media supplemented with 0.085 M NaCl and 0.05 M MgCl2. Phage replication occurred in cultures of Aeromonas sp. growing under applied hydrostatic pressures of up to 200 atmospheres. Electron microscopy revealed that the phage has an hexagonal head 530 Å in diameter, a thin tail 1200 Å in length and a terminal base plate 400 Å in diameter.
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Communicated by G.L. Voss, Miami
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Wiebe, W.J., Liston, J. Isolation and characterization of a marine bacteriophage. Marine Biol. 1, 244–249 (1968). https://doi.org/10.1007/BF00347117
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DOI: https://doi.org/10.1007/BF00347117