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Oligonucleotide fingerprinting detects genetic diversity among Ascochyta rabiei isolates from a single chickpea field in Tunisia

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Abstract

Fifty isolates of Ascochyta rabiei (Pass.) Labr. were hierarchically sampled from four well-separated locations of a single chickpea field in Beja (Tunisia), and single-spored. DNA was isolated from in-vitro-grown mycelia, digested with HinfI or RsaI, and hybridized to a set of synthetic oligonucleotides complementary to simple repetitive sequences. According to the fingerprint patterns derived from the probes (CA)8, (CAA)5, (CAT)5 and (GATA)4, 12 different fungal haplotypes were found at various frequencies within the investigated field. Seven haplotypes were confined to one location only, four occurred at two, one at three, and none at all four locations. Most of the genetic variability originated from diversity within, rather than between, locations. In some cases, more than one haplotype was isolated from the same lesion of a single host plant. Genetic distances between isolates, as calculated from band-sharing data, varied between 0.05 and 0.22. Relatedness between the different haplotypes was evaluated by cluster analysis using UPGMA.

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References

  • Alam SS, Bitton JN, Slawin AMZ, Williams RN, Shepard RN, Strange RN (1989) Chickpea blight: production of the phytotoxins solanapyron a and c by Ascochyta rabiei. Phytochemistry 28: 2627–2631

    Google Scholar 

  • Ali S, Müller CR, Epplen JT (1986) DNA fingerprinting by oligonucleotide probes specific for simple repeats Hum. Genet, 74: 239–243

    Google Scholar 

  • Anagnostakis SL, Kranz J (1987) Population dynamics of Cryphonectria parasitica in a mixed-hardwood forest in Connecticut. Phytopathology 77:751–754

    Google Scholar 

  • Bierwerth S, Kahl G, Weigand F, Weising K (1992) Oligonucleotide fingerprinting of plant and fungal genomes: a comparison of radioactive, colorigenic and chemiluminescent detection methods. Electrophoresis 13:115–122

    Google Scholar 

  • Correll JC, Puhalla JE, Schneider RW (1986) Vegetative compatibility groups among non-pathogenic root-colonizing strains of Fusarium oxysporum. Can J Bot 64:2358–2361

    Google Scholar 

  • Crowhurst RN, Hawthorne BT, Rikkerink EHA, Templeton MD (1991) Differentiation of Fusarium solanii f. sp. curcurbitae races 1 and 2 by random amplification of polymorphic DNA. Curr Genet 20:391–396

    Google Scholar 

  • Drenth A, Goodwin SB, Fry WE, David LC (1993) Genotypic diversity of Phytophthora infestans in the Netherlands revealed by DNA polymorphisms. Phytopathology 83:1087–1092

    Google Scholar 

  • Felsenstein J (1988) Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 22:521–565

    Google Scholar 

  • Goodwin PH, Annis SL (1991) Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by RAPDNA assay. Appl Environ Microbiol 57:2482–2486

    Google Scholar 

  • Grewal JS (1984) Evidence of physiological races in Ascochyta rabiei of chickpea. In Saxena C, Singh KB (eds) Ascochyta blight and winter sowing of chickpea. Martinus Nijhoff, The Hague, Netherlands, pp 55–65

    Google Scholar 

  • Hamer JE, Farrall L, Orbach MJ, Valent B, Chumley FG (1989) Hostspecies-specific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen. Proc Natl Acad Sci USA 86:9981–9985

    Google Scholar 

  • Höhl B, Weidemann C, Höhl U, Barz W (1991) Isolation of solanapyrones a, b and c from culture filtrates and germination fluids of Ascochyta rabiei and aspects of phytotoxin action. J Phytopathol 132:193–206

    Google Scholar 

  • Jan H, Wiese MV (1991) Virulence forms of Ascochyta rabiei affecting chickpea in the palouse. Plant Disease 75:904–906

    Google Scholar 

  • Kaemmer D, Ramser J, Schön M, Weigand F, Saxena MC, Driesel AC, Kahl G, Weising K (1992) DNA fingerprinting of fungal genomes: a case study with Ascochyta rabiei. BTF. Adv Mol Genet 5:255–270

    Google Scholar 

  • Kaiser WJ (1973) Factors affecting growth, sporulation, pathogenicity and survival of Ascochyta rabiei. Mycologia 65:444–447

    Google Scholar 

  • Kaiser WJ (1992) Epidemiology of Ascochyta rabiei. In Singh KB, Saxena MC (eds) Disease resistance in chickpea. ICARDA, Aleppo, Syria, pp 117–134

    Google Scholar 

  • Kistler HC, Momol EA, Benny U (1991) Repetitive genomic sequences for determining relatedness among strains of Fusarium oxysporum. Phytopathology 81:331–336

    Google Scholar 

  • Kohn LM, Petsche DM, Bailey SR, Novak LA, Anderson JB (1988) Mycelial incompatibility and molecular markers identify genetic variability in field populations of Sclerotina scleroticum. Phytopathology 78:1047–1051

    Google Scholar 

  • Leuchtmann A, Clay K (1989) Isozyme variations in the Acremonium/Epichloe fungal endophyte complex. Can J Bot 67:2600–2607

    Google Scholar 

  • Levy M, Romao J, Marchetti MA, Hamer JE (1991) DNA fingerprinting with dispersed repeated sequences resolves pathotype diversity in the rice blast fungus. Plant Cell 3:95–102

    Google Scholar 

  • McDermott JM, McDonald BA, Allard RW, Webster RK (1989) Genetic variability for pathogenicity, isozyme, ribosomal DNA and colony color variants in populations of Rhynchosporium secalis. Genetics 122:561–565

    Google Scholar 

  • McDonald BA, Martinez JP (1990) DNA restriction fragment length polymorphisms among Magnaporthe graminicola (anamorph Septoria tritici) isolates collected from a single wheat field. Phytopathology 80:1368–1373

    Google Scholar 

  • McDonald BA, Martinez JP (1991) DNA fingerprinting of the plantpathogenic fungus Magnaporthe graminicola (anamorph Septoria tritici). Exp Mycol 15:146–158

    Google Scholar 

  • McDonald BA, McDermott JM, Goodwin SB, Allard RW (1989a) The population biology of host-pathogen interactions. Annu Rev Phytopathol 27:77–94

    Google Scholar 

  • McDonald BA, McDermott JM, Allard RW, Webster RK (1989b) Coevolution of host-pathogen populations in the Hordeum vulgare/Rhychosporium secalis pathosystem. Proc Natl Acad Sci USA 86:3924–3927

    Google Scholar 

  • Meyer W, Koch A, Niemann C, Beyermann B, Epplen JT, Börner T (1991) Differentiation of species and strains among filamentous fungi by DNA fingerprinting. Curr Genet 19:239–242

    Google Scholar 

  • Meyer W, Morawetz R, Börner T, Kubicek CP (1992a) The use of DNA fingerprinting analysis in classification of some species of the Trichoderma aggregate. Curr Genet 21:27–30

    Google Scholar 

  • Meyer W, Lieckfeldt E, Wöstemeyer J, Börner T (1992b) DNA fingerprinting for differentiating aggressivity groups of the rape seed pathogen Leptosphaeria maculans. Mycol Res 96:651–657

    Google Scholar 

  • Milgroom MG, Lipari SE, Powell WA (1992a) DNA fingerprinting and analysis of population structure in the chestnut blight fungus Cryphonectria parasitica. Genetics 131:297–306

    Google Scholar 

  • Milgroom MG, Lipari SE, Wang K (1992b) Comparison of genetic diversity in the chestnut blight fungus Cryphonectria parasitica from China and the US. Mycol Res 96:1114–1120

    Google Scholar 

  • Nei (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323

    Google Scholar 

  • Nene YL, Reddy MV (1987) Chickpea Diseases and their Control. In: Saxena MC, Singh KB (eds), The chickpea. CAB International, Wallingford, UK, pp 233–270

    Google Scholar 

  • Pena SDJ, Chakraborty R, Epplen JT, Jeffreys AJ (1993) DNA fingerprinting: state of the science. Birkhäuser, Basel

    Google Scholar 

  • Porta-Puglia A (1992) Variability in Ascochyta rabiei. In: Singh KB, Saxena MC (eds) Disease resistance in chickpea. ICARDA, Aleppo, Syria, pp 135–143

    Google Scholar 

  • Rodriguez RJ, Yoder OC (1991) A family of conserved repetitive DNA elements from the fungal plant pathogen Glomerella cingulata f. sp. lindemuthianum. Exp Mycol 15:232–242

    Google Scholar 

  • Saxena MC, Singh KB (1987). The chickpea. CAB International, Wallingford, UK

    Google Scholar 

  • Schäfer C, Wöstemeyer J (1992) Random-primer-dependent PCR differentiates aggressive from non-aggressive isolates of the oilseed rape pathogen Phoma lingam (Leptosphaeria maculans). J Phytopathol 136:124–136

    Google Scholar 

  • Schäfer R, Zischler H, Birsner U, Becker A, Epplen JT (1988) Optimized oligonucleotide probes for DNA fingerprinting. Electrophoresis 9:369–374

    Google Scholar 

  • Singh KB, Saxena MC (1992) Disease resistance in chickpea. ICARDA, Aleppo, Syria

    Google Scholar 

  • Sneath PHA, Sokal RR (1973) Numerical taxonomy. Freeman, San Fancisco

    Google Scholar 

  • Thein SL, Wallace RB (1986) The use of synthetic oligonucleotides as specific hybridization probes in the diagnosis of genetic disorders. In: Davies KE (ed) Human genetic diseases—a practical approach. IRL Press, Oxford, pp 33–50

    Google Scholar 

  • Trapero-Casas A, Kaiser WJ (1992) Development of Didymella rabiei, the teleomorph of Ascochyta rabiei, on chickpea straw. Phytopathology 82:1261–1266

    Google Scholar 

  • Van de Peer Y, De Wachter R (1993) Treecon, a software package for the construction and drawing of evolutionary trees. Comput Applic Biosci 9:177–182

    Google Scholar 

  • Weising K, Kaemmer D, Epplen JT, Weigand F, Saxena MC, Kahl G (1991) DNA fingerprinting of Ascochyta rabiei with synthetic oligodeoxynucleotides. Curr Genet 19:483–489

    Google Scholar 

  • Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218

    Google Scholar 

  • Williams JGK, Kubelik AR, Livak AR, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Google Scholar 

  • Xia JQ, Correll JC, Lee FN, Marchetti MA, Rhoads DD (1993) DNA fingerprinting to examine microgeographic variation in the Magnaporthe grisea population in two rice fields in Arkansas. Phytopathology 83:1029–1035

    Google Scholar 

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Authors and Affiliations

  1. Plant Molecular Biology Group, Biozentrum, University of Frankfurt am Main, D-60439, Frankfurt, Germany

    Hichem Morjane, Jörg Geistlinger, Kurt Weising & Günter Kahl

  2. Institut National Agronomique de Tunis (INAT), 43 Av. Charles Nicolles, 1082, Cité Mahrajene, Tunisia

    Hichem Morjane & Moncef Harrabi

Authors
  1. Hichem Morjane
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  2. Jörg Geistlinger
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  3. Moncef Harrabi
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  4. Kurt Weising
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  5. Günter Kahl
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Morjane, H., Geistlinger, J., Harrabi, M. et al. Oligonucleotide fingerprinting detects genetic diversity among Ascochyta rabiei isolates from a single chickpea field in Tunisia. Curr Genet 26, 191–197 (1994). https://doi.org/10.1007/BF00309547

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  • DOI: https://doi.org/10.1007/BF00309547

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