Summary
Enhancing factor (EF), a 14 kDa protein, isolated from mouse small intestines, has been reported from this laboratory. Based on our earlier studies EF has been implicated in cell proliferation. Preliminary immunohistochemical studies have shown EF to be localized in the Paneth cells of small intestines. In this paper we report the tissue distribution of EF using conditions optimized for immunohistochemical staining. In addition, the data are supported by northern blot analysis using a nick translated cDNA probe specific for EF. The results indicate that EF gene is actively transcribed mainly in the intestines. The chief source of synthesis of EF appears to be the Paneth cells located at the base of the crypts of Lieberkühn.
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References
Creamer B (1967) Paneth cell function. Lancet I:314–316
Deo MG, Mulherkar R, Mane SM (1983) Isolation of a polypeptide that enhances cellular binding of epidermal growth factor. Ind J Biochem Biophys 20:228–231
Gordon JI (1989) Intestinal epithelial differentiation: new insights from chimeric and transgenic mice. J Cell Biol 108:1187–1194
Mulherkar R, Deo MG (1986a) Further studies on the enhancing factor and its possible mechanism of action. J Cell Physiol 127:183–188
Mulherkar R, Saraf A, Wagle AS, Deo MG (1986b) Purification of the enhancing factor from mouse intestines. FEBS Lett 207:142–144
Peeters T, Vantrappen G (1975) The Paneth cell: a source of intestinal lysozyme. Gut 16:553–558
Poulson SS, Nexo E, Olsen PS, Hess J, Kirkegard P (1986) Immunohistochemical localization of EGF in rat and man. Histochemistry 85:389–394
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. CSHL, New York
Shimasaki S, Emoto N, Koba A, Mercado M, Shibata F, Cooksey K, Baird A, Ling N (1988) Biochem Biophys Res Commun 157:256–263
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Wagle A, Desai S, Deo MG (1988) Immunohistochemical localization of ‘Enhancing Factor’, a novel growth modulator in the mouse small intestines. Cell Biol Int Rep 13:309–312
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Mulherkar, R., Desai, S.J., Rao, R.S. et al. Expression of enhancing factor gene and its localization in mouse tissues. Histochemistry 96, 367–370 (1991). https://doi.org/10.1007/BF00271358
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DOI: https://doi.org/10.1007/BF00271358