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Determination of glomerular filtration rate (GFR) by analysis of capillary blood after single shot injection of 99mTc-DTPA

A comparison with simultaneous 125I-Iothalamate GFR estimation showing equal GFR but difference in distribution volume

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Abstract

99mTc-DTPA was prepared from a kit produced by the Institute of Atomic Energy, Oslo, Norway. Radiochemical purity as determined with gel chromatography ranged from 98.5–99.7% (n=7). The radiopharmaceutical showed no red cell uptake and not more than 0.2% protein binding in in vitro biokinetic studies.

The clearance of 99mTc-DTPA was compared to the clearance of 125I-Iothalamate simultaneously using single shot intravenous injection and biexponential analysis of plasma activity disappearance rate according to Sapirstein et al. (1955). 125I-Iothalamate was found to have a higher second volume of distribution than 99mTc-DTPA, but there was no statistically significant difference in clearance.

GFR calculated from capillary serum 99mTc-DTPA count rates was in all subjects investigated virtually identical with GFR calculated from simultaneously collected venous plasma samples.

Estimation of GFR on the basis of plasma activity curves obtained from sampling in two hours gave higher values than estimation from four hours sampling irrespective of kidney function and whether 99mTc-DTPA or 125I-Iothalamate was used.

It is concluded that 99mTc is almost entirely bound to DTPA after intravenous injection of the 99mTc-DTPA complex, and that the complex is a suitable agent for determination of glomerular filtration rate, using both venous and capillary blood sampling.

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Rootwelt, K., Falch, D. & Sjökvist, R. Determination of glomerular filtration rate (GFR) by analysis of capillary blood after single shot injection of 99mTc-DTPA. Eur J Nucl Med 5, 97–102 (1980). https://doi.org/10.1007/BF00252466

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