Skip to main content
Log in

Correlation between classification of human urothelial cell lines and HLA-A,B,C expression

  • Original Articles
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Summary

Quantitative changes in major histocompatibility class I antigen expression in tumour cells are believed to affect the host immune response against the tumour. In tumourigenic (TGrIII) human urothelial cell lines the apparent loss of polymorphic HLA-A,B epitopes has previously been demonstrated. In the present study, 3 non-tumourigenic (TGrII) and 6 tumourigenic (TGrIII) human urothelial cell lines have been investigated for their quantitative expression of monomorphic HLA-A,B,C and B2-microglobulin. Evidence is provided that an inverse correlation exists between tumourigenicity and HLA-A,B,C and B2-microglobulin expression. Furthermore, treatment of the cells with neuraminidase partly restored the expression of monomorphic HLA-A,B,C suggesting that at least some of the observed quantitative differences could be due to masking of the membrane bound HLA antigens by sialic acid-containing glycoconjugates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Debray H, Zhu Q, Delanny P, Montreuil J, Dus D, Radzikowski C, Christensen B, Kieler J (1986) Altered glycosylation of membrane glycoproteins in human uroepithelial cell lines. Int J Cancer 37:602–612

    Google Scholar 

  2. Christensen B, Kieler J, Vilien M, Don M, Wang CY, Wolf R (1984) A classification of human urothelial cells propagated in vitro. Anticancer Res 4:319–338

    Google Scholar 

  3. Doherty PC, Knowles BB, Wettstein PJ (1984) Immunological surveillance of tumours in the context of major histocompatibility complex restriction of T-cell function. Adv Cancer Res 42:1–65

    Google Scholar 

  4. Doyle A, Martin WJ, Funa R, Gazdar A, Carney D, Martin SE, Linnoila I, Cuttitta F, Mulshine J, Bunn P, Minna J (1985) Marked decreased expression of Class I histocompatibility antigens, proteins, and mRNA in human small-cell lung cancer. J Exp Med 161:1135–1151

    Google Scholar 

  5. Festenstein H, Garrido F (1986) MHC antigens and malignancy. Nature 322:502–503

    Google Scholar 

  6. Goodenow RS, Vogel JM, Linsk RL (1985) Histocompatibility antigens on murine tumours. Science 230:777–783

    Google Scholar 

  7. Hicks RM, Wakefield JSTJ (1976) Membrane changes during urothelial hyperplasia and neoplasia. Cancer Res 36:2502–2507

    Google Scholar 

  8. Ingh HF, Ruiter DJ, Griffioen G, Muijen GNP, Ferrone S (1987) HLA antigens in colorectal tumours — low expression of HLA Class I antigens in mucinous colorectal carcinomas. Br J Cancer 55:125–130

    Google Scholar 

  9. Johnson GD, Nogueira Araujo GMC (1981) A simple method of reducing fading of immunofluorescence during microscopy. J Immunol Methods 43:349–350

    Article  CAS  PubMed  Google Scholar 

  10. Kieler J (1984) Invasiveness of transformed bladder epithelial cells. Cancer Metas Rev 3:265–296

    Google Scholar 

  11. Lampson LA, Fisher CA, Whelan J (1983) Striking paucity of HLA-A,B,C and B2-microglobulin on human neuroblastoma cell lines. J Immunol 130:2471–2478

    Google Scholar 

  12. Momburg F, Degener T, Bacchus E, Moldenhauer G, Hammerling GJ, Moller P (1986) Loss of HLA-A,B,C and de novo expression of HLA-D in colorectal cancer. Int J Cancer 37:179–184

    Google Scholar 

  13. Natali PG, Bigotti A, Nicotra MR, Viora M, Manfred D, Ferrone S (1984) Distribution of human Class I (HLA-A,B,C) histocompatibility antigens in normal and malignant tissues of nonlymphoid origin. Cancer Res 44:4679–4687

    Google Scholar 

  14. Ottesen SS, Kieler J, Christensen B (1987) Changes in HLA-A,B,C expression during “spontaneous” transformation of human urothelial cells in vitro. Eur J Cancer Clin Oncol 23:991–995

    Google Scholar 

  15. Parham P, Barnstable CJ, Bodmer WF (1979) Use of a monoclonal antibody (W6/32) in structural studies of HLA-A,B,C antigens. J Immunol 123:341–349

    Google Scholar 

  16. Stroynowski I, Clark S, Henderson LA, Hood L, McMillan M, Forman J (1985) Interaction of 01 with 02 region in Class I MHC proteins contributes determinants recognized by antibodies and cytotoxic T cells. J Immunol 135:2160–2166

    Google Scholar 

  17. Trowssdale J, Travers P, Bodmer F, Patillo RA (1980) Expression of HLA-A,B,C and B2-microglobulin antigens in human choriocarcinoma cell lines. J Exp Med 152:11s-17s

    Google Scholar 

  18. Vilien M, Wolf H, Rasmussen F (1981) Immunological characterization of cell lines established from malignant and normal human urothelium. Eur J Cancer 17:321–327

    Google Scholar 

  19. Vilien M, Christensen B, Wolf H, Rasmussen F, Hou-Jensen C, Povlsen CO (1983) Comparative studies of normal, “spontaneously” transformed and malignant urothelium cells in vitro. Eur Cancer Clin Oncol 19:775–789

    Google Scholar 

  20. Walton GR, McCue PA, Graham Jr SD (1986) Beta-2 microglobulin as a differentiation marker in bladder cancer. J Urol 136:1197–1200

    Google Scholar 

  21. Yogeeswaran G, Salk PL (1981) Metastatic potential is positively correlated with cell surface sialylation of cultured murine tumor cell lines. Science 212:1514–1516

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ottesen, S.S., Tromholt, V. & Kieler, J. Correlation between classification of human urothelial cell lines and HLA-A,B,C expression. Cancer Immunol Immunother 26, 83–86 (1988). https://doi.org/10.1007/BF00199852

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00199852

Keywords

Navigation