Abstract
Stimulation of leaf expansion by an exogenous cytokinin was studied in isolated leaf discs of sweet pepper with emphasis on the assimilate utilization of the tissue. Leaf discs were floated on solutions containing sucrose and plant growth regulators. Benzyladenine (BA) promoted the area expansion rate of the leaf discs. Sucrose at 100 mM resulted in increased area expansion rate compared with 10 mM sucrose. However, the increased sucrose concentration had no influence on the effect of BA. Over a period of 24 h, treatment with BA did not result in any change of sucrose uptake nor of the partitioning of assimilated carbon in the leaf discs. Neither did BA treatment affect the activity of acid invertase (EC 3.2.1.26) or pyrophosphate-dependent phosphofructokinase (EC 2.7.1.90) in the leaf discs. We conclude that the observed promotion of leaf area expansion by exogenous BA is not mediated through the uptake of sucrose or the carbohydrate metabolism of the leaf tissue.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BA:
-
N6-benzyladenine
- GA3 :
-
gibberellic acid
- PPi-PFK:
-
pyrophosphate-dependent phosphofructokinase (EC 2.7.1.90)
References
Aloni, B., Pashkar, T., Kami, L. (1991) Partitioning of [14C]sucrose and acid invertase activity in reproductive organs of pepper plants in relation to their abscission under heat stress. Ann. Bot. 67, 371–377
Brock, T.G., Cleland, R.E. (1990) Biophysical basis of growth promotion in primary leaves of Phaseolus vulgaris L. by hormones versus light. Solute accumulation and the growth potential. Planta 182, 427–431
Dale, J.E. (1988) The control of leaf expansion. Annu. Rev. Plant Physiol. Plant Mol. Biol. 39, 267–295
Goodwin, P.B., Erwee, M.G. (1983) Hormonal influences on leaf growth. In: The growth and functioning of leaves, pp. 207–232, Dale, J.E., Milthorpe, F.L., eds. Cambridge University Press, Cambridge, UK
Hall, A.J. (1977) Assimilate source-sink relationship in Capsicum annuum L. I. The dynamics of growth in fruiting and deflorated plants. Aust. J. Plant. Physiol. 4, 623–636
Hewett, E.W., Wareing, P.F. (1973) Cytokinins in Populus x robusta (Schneid): Light effects on endogenous levels. Planta 114, 119–129
Ho, L.C. (1988) Metabolism and compartmentation of imported sugars in sink organs in relation to sink strength. Annu. Rev. Plant Physiol. 39, 335–378
Labavitch, J.M., Ray, P.M. (1974a) Turnover of cell wall polysaccharides in elongating pea stem segments. Plant Physiol. 53, 669–673
Labavitch, J.M., Ray, P.M. (1974b) Relationship between promotion of xyloglucan metabolism and induction of elongation by indoleacetic acid. Plant Physiol. 54, 499–502
Leopold, A.C., Kawase, M. (1964) Benzyladenine effects on bean leaf growth and senescence. Am. J. Bot. 51, 294–298
Letham, D.S. (1971) Regulators in cell division in plant tissues. XII. A cytokinin bioassay using excised radish cotyledons. Physiol. Plant. 25, 391–396
Mothes, K. (1961) Der Beitrag der Kinetinforchung zum Verständnis pflanzlicher Korrelationen. Ber. Dtsch. Bot. Ges. 74, 24–41
Nielsen, T.H. (1987) Regulering af kulstoffordeling i sød peber (Capsicum annuum L.). Betydning af udviklingsstadie og fruc-tose 2,6-bisphosphate. Ph.D. thesis, Department of Plant Physiology, Royal Veterinary and Agricultural University, Denmark
Nielsen, T.H. (1990) Enzymology of the carbohydrate metabolism during leaf development in sweet pepper. Physiol. Plant. 79, A131, abstract 749
Nielsen, T.H. Veierskov, B. (1990) Regulation of carbon partitioning in source and sink leaf parts in sweet pepper (Capsicum annuum L.) plants. Role of fructose 2,6-bisphosphate. Plant Physiol. 93, 637–641
Nielsen, T.H., Skjærbæk, H.C., Karlsen, P.K. (1991) Carbohydrate metabolism during fruit development in sweet Pepper (Capsicum annuum) plants. Physiol. Plant. 82, 311–319
Nishitani, K., Masuda, Y. (1981) Auxin-induced changes in the cell wall structure: Changes in the sugar composition, intrisic viscosity and molecular weight distributions of matrix polysaccharides of the epicotyl cell wall of Vigna angularis. Physiol. Plant. 52, 482–494
Salisbury, F., Ross, C.W. (1985) Plant physiology, 3rd edn., pp. 334–335, Wadsworth Inc., Belmont, California
Stiebling, B., Neumann, K.-H. (1987) Identification and concentration of endogenous cytokinins in carrots (Daucus carota L.) as influenced by development and circadian rhythm. J. Plant Physiol. 127, 111–121
Terry, N., Waldron, L.J., Taylor, S.E., (1983) Environmental influences on leaf growth. In: The growth and functioning of leaves, pp. 179–205, Dale, J.E., Milthorpe, F.L., eds. Cambridge University Press, Cambridge
Thompson, A.G., Horgan, R., Heald, J.K. (1975) A quantitative analysis of cytokinin using single-ion-current-monitoring. Planta 124, 207–210
Turgeon, R. (1989) The sink-source transition in leaves. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 119–138
Ulvskov, P., Nielsen, T.H., Seiden, P., Marcussen, J. (1992) Cytokinins and leaf development in sweet pepper (Capsicum annuum L.). I. Spatial distribution of endogenous cytokinins in relation to leaf growth. Planta
Volkenburg, E.V., Cleland, R.E. (1990) Light-stimulated cell expansion in bean (Phaseolus vulgaris L.) leaves. I. Growth can occur without photosynthesis. Planta 182, 72–76
Author information
Authors and Affiliations
Additional information
This study was supported by grants from the Danish Research Counsil (SJVF 13-4148 and 13-4547 to P.U. SJVF 13-4146 and 13-4494 to T.H.N.) and from The Research Center for Plant Biotechnology to P.U.
Rights and permissions
About this article
Cite this article
Nielsen, T.H., Ulvskov, P. Cytokinins and leaf development in sweet pepper (Capsicum annuum L.). Planta 188, 78–84 (1992). https://doi.org/10.1007/BF00198942
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00198942