Abstract
The isolation and fusion of protoplasts in several species of Porphyra is described. Protoplasts from all species in the present study were obtained by treating thalli with commercial protease and bacterial crude enzyme prepared in the laboratory. Protoplast fusion was accomplished by following polyethylene glycol (PEG) and electrofusion methods. In the electrofusion method, protoplasts aligned in an AC field (1 MHz, 40 V for 20 s) and subsequently fused with a DC pulse of 250 V for 40 µs yielded optimum (about 20%) binary or trinary fusion products compared to the PEG method (about 8–10%). Chromosome counts and absorption spectra of crude phycobilins of most regenerated plants were identical to one or the other of the parental types, but several chimeral thalli showed a mixture of chromosome numbers and pigmentation.
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Fujita, Y., Saito, M. Protoplast isolation and fusion in Porphyra (Bangiales, Rhodophyta). Hydrobiologia 204, 161–166 (1990). https://doi.org/10.1007/BF00040228
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DOI: https://doi.org/10.1007/BF00040228