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Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis & Management pp 143–167Cite as

Detection of Seed and Propagating Material-Borne Bacterial Diseases of Economically Important Crops

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Abstract

Seeds and propagating materials of plants are the primary source of pathogen inoculum to cause diseases. These materials also transmit the pathogen from one place to other places and establish the disease in a new area. Hence, it is an utmost requirement to detect the pathogen of a particular crop before transportation and sowing to ensure that no potentially damaging pathogens are introduced in the field through seeds and planting materials. This can be most effectively accomplished by keeping out pathogens from seed lots by either discarding or treating seeds with chemicals. Various conventional methods for the detection of pathogens such as visual examination, selective growth media, serological methods, and bioassay have been used commonly. But these methods have disadvantages like inefficiency, less specificity, less sensitivity, and more time-consuming. Now-a-days, polymerase chain reaction (PCR) has more potential to improve bacterial pathogen detection in seeds as well as planting materials. There are advanced techniques like BIO-PCR, immunomagnetic separation-PCR (IMS-PCR), and magnetic capture hybridization-PCR (MCH-PCR) which reduce inhibitory compounds during PCR, which further improve the detection level of bacterial pathogens from seeds and planting materials. IMS-PCR and MCH-PCR are more attractive due to their simple and universally applicable methods to test seeds for different culturable and non-culturable bacterial pathogens. However, it is difficult to adapt their applicability for routine testing of seed under the laboratory. It should be ensured that these methods should work and these methods must be validated in multi-laboratory tests thoroughly and these tests should be reproducible and repeatable before their commercialization.

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  1. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Dinesh Singh & Priyanka Singh Rathaur

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  1. ICAR-Indian Agricultural Research Institute, Regional Station, Karnal, Haryana, India

    Ravindra Kumar

  2. ICAR-Indian Agricultural Research Institute, Regional Station, Karnal, Haryana, India

    Anuja Gupta

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Singh, D., Rathaur, P.S. (2020). Detection of Seed and Propagating Material-Borne Bacterial Diseases of Economically Important Crops. In: Kumar, R., Gupta, A. (eds) Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis & Management. Springer, Singapore. https://doi.org/10.1007/978-981-32-9046-4_6

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