Skip to main content

Recent Updates on Molecular Biotechnological Intervention in Isabgol

  • Chapter
  • First Online:

Abstract

Isabgol is a very important native medicinal plant and export commodity of India, which is the largest producer. At this stage, it becomes very important to study the genetic diversity within isabgol gene pool for proper maintenance, conservation, validation, and effective utilization. There are rare reports of DNA fingerprinting of Plantago ovata genotypes for determining genetic diversity or varietal identification. In this chapter, we discuss about the role of DNA markers especially random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSRs) for genetic diversity study for useful crops by taking isabgol as a case study. No other reports of recent markers are available for isabgol. Genetic variation in Plantago species is of prime significance for focusing on the constructive breeding of improved varieties/cultivars with added value and durable resistance against diseases.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Adams RP, Andrea E, Schwarzbach R, Pandey N (2003) The concordance of terpenoid, ISSR and RAPD markers and ITS sequence data sets among genotypes: an example from Juniperus. Biochem Syst Ecol 31:375–387

    Article  CAS  Google Scholar 

  • Akagi H, Yokozeki Y, Inagaki A, Nakamura A, Fujimura T (1996) A codominant DNA marker closely linked to the rice nuclear restorer gene, Rf-1, identified with inter-SSR fingerprinting. Genome 39:1205–1209

    Article  CAS  PubMed  Google Scholar 

  • Ammiraju JSS, Dholakia BB, Santra DK, Singh H et al (2001) Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat. Theor Appl Genet 102:726–732

    Article  CAS  Google Scholar 

  • Ana A, Bandini R, Luiz Orlando de O, Bruna AV, Silva RS (2009) Genetic diversity and geographic differentiation of disjunct Atlantic and Amazonian populations of Psychotriai pecacuanha (Rubiaceae). Genetica 136:57–67

    Article  Google Scholar 

  • Awasthi AK, Nagaraja GM, Naik GV et al (2004) Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays. BMC Genet 5:100–104

    Article  Google Scholar 

  • Banerjee H, Pai RA, Moss JP, Sharma RP (2001) Use of random amplified polymorphic DNA markers for mapping the chickpea genome. Biol Plant 44:195–202

    Article  CAS  Google Scholar 

  • Baranger A, Aubert G, Arnau G, Lainé AL et al (2004) Genetic diversity within Pisum sativum using protein and PCR-based markers. Theor Appl Genet 108:1309–1321

    Article  CAS  PubMed  Google Scholar 

  • Beebe S, Skroch PW, Tohme J et al (2000) Structure of genetic diversity among common bean landraces of Middle American origin based on correspondence analysis of RAPD. Crop Sci 40:264–273

    Article  Google Scholar 

  • Behera TK, Singh AK, Staub JE (2008) Comparative analysis of genetic diversity in Indian bitter gourd (Momordica charantia L.) using RAPD and ISSR markers for developing crop improvement strategies. Sci Hortic 115:209–217

    Article  CAS  Google Scholar 

  • Bhatia R, Singh KP, Jhang T, Sharma TR (2009) Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers. Sci Hortic 119:208–211

    Article  CAS  Google Scholar 

  • Blair MW, Panaud O, McCouch SR (1999) Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L). Theor Appl Genet 98:780–792

    Article  CAS  Google Scholar 

  • Carvalho A, Lima-Brito J, Ma ÃÃB, Guedes-Pinto H (2009) Genetic diversity and variation among botanical varieties of old Portuguese wheat cultivars revealed by ISSR assays. Biochem Genet 47:276–294

    Article  CAS  PubMed  Google Scholar 

  • Casu M, Tiziana L, Marco CG, Alberto R, Antonio P (2009) Identification of Mediterranean Diplodus spp and Dentex dentex (Sparidae) by means of DNA Inter-Simple Sequence Repeat (ISSR) markers. J Exp Mar Biol Ecol 368:147–152

    Article  CAS  Google Scholar 

  • Causse MA, Fulton TM, Cho YG, Alin SN et al (1994) Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138:1251–1274

    CAS  PubMed  PubMed Central  Google Scholar 

  • Chopra RN (1930) Plantago ovata (Isbaghul) in chronic diarrhoeas and dysenteries. Ind Med Gaz 65:428–433

    Google Scholar 

  • Dallas JF (1988) Detection of DNA fingerprints of cultivated rice by hybridization with a human minisatellite DNA probe. Proc Natl Acad Sci U S A 85:6831–6835

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Das M, Raychaudhuri SS (2003) Estimation of genetic variability in Plantago ovata cultivars. Biol Plant 47(3):459–462

    Google Scholar 

  • De Bustos, Casanova A, Soler C, Jouve N (1998) RAPD variation in wild populations of four species of the genus Hordeum (Poaceae). Theor Appl Genet 96:101–111

    Article  CAS  Google Scholar 

  • Devos KM, Gale MD (1992) The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84:567–572

    CAS  PubMed  Google Scholar 

  • Dhar MK, Kaul S, Friebe B, Gill BS (2002) Chromosome identification in Plantago ovata Forsk through C-banding and FISH. Curr Sci 83(2):150–152

    CAS  Google Scholar 

  • Dorweilar J, Stec A, Kermiele J, Doebley J (1993) Teosinte glune architecture I: a genetic locus controlling a key step in maize evaluation. Science 262:233–235

    Article  Google Scholar 

  • Duarte JM, Santos JB, Melo LC (1999) Genetic divergence among common bean cultivars from different races based on RAPD markers. Genet Mol Biol 22:419–426

    Article  Google Scholar 

  • Flowers TJ, Koyama ML, Flowers SA et al (2000) QTL: their place in engineering tolerance of rice to salinity. J Exp Bot 51:99–106

    Article  CAS  PubMed  Google Scholar 

  • Fofana B, Vekemans X, Jardin P, Baudoin JP (1997) Genetic diversity in lima bean (Phaseolus lunatus L.) as revealed by RAPD markers. Euphytica 95:157–165

    Article  CAS  Google Scholar 

  • French D, Wild GM, Young, James WJ (1953) Constitution of plantiose. J Am Chem Soc 75:709–712

    Article  CAS  Google Scholar 

  • Gale MD, Witcombe JR (1992) DNA markers and marker-mediated applications in plant breeding with particular reference to pearl millet breeding. In: Moss JP (ed) Biotechnology and crop improvement in Asia, ICRISAT, Patancheru, AP 502324, India, pp 323–332

    Google Scholar 

  • Galvan MZ, Bornet B, Balatti PA, Branchard M (2003) Inter simple sequence repeat (ISSR) markers as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L). Euphytica 132:297–301

    Article  CAS  Google Scholar 

  • Geleta M, Bryngelsson T (2009) Inter simple sequence repeat (ISSR) based analysis of genetic diversity of Lobelia rhynchopetalum (Campanulaceae). Hereditas 146:122–130

    Article  PubMed  Google Scholar 

  • Goulão L, Oliveira CM (2001) Molecular characterization of cultivars of apple (Malus×domestica Borkh) using microsatellite (SSR, ISSR) markers. Euphytica 122:81–89

    Article  Google Scholar 

  • Gulsen O, Sever-Mutlu S, Mutlu N, Tuna M et al (2009) Polyploidy creates higher diversity among Cynodon accessions as assessed by molecular markers. Theor Appl Genet 118:1309–1319

    Article  CAS  PubMed  Google Scholar 

  • Gupta M, Chyi YS, Romero-Severson J, Owen JL (1994) Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats. Theor Appl Genet 89:998–1006

    CAS  PubMed  Google Scholar 

  • Han Y, Wang HY (2010) Genetic diversity and phylogenetic relationships of two closely related northeast China Vicia species revealed with RAPD and ISSR markers. Biochem Genet 48:385–401

    Article  CAS  PubMed  Google Scholar 

  • Hongyan S, Wang L, Liu L, Chi X, Zhang Y (2008) Use of inter-simple sequence repeats markers to develop strain-specific SCAR markers for Flammulina velutipes. J Appl Genet 49(3):233–235

    Article  Google Scholar 

  • Hou L, Hongli L, Zou L et al (2006) Genetic characterizations of Mactra veneriformis (Bivalve) along the Chinese coast using ISSR-PCR markers. Aquaculture 261:865–871

    Article  CAS  Google Scholar 

  • Hu J, Quiros CF (1991) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep 10:505–511

    Article  CAS  PubMed  Google Scholar 

  • Hyde BB (1970) Mucilage producing cells in the seed coat of Plantago ovata: development fine structure. Am J Bot 57(10):1197–1206

    Article  Google Scholar 

  • Isshiki S, Iwata N, Mizanur M, Khan R (2008) ISSR variations in eggplant (Solanum melongena L.) and related Solanum species. Sci Hortic 117:186–190

    Article  CAS  Google Scholar 

  • Jamal SI, Ahmad R, Agrawal M, Ahamed OSM (1987) A novel oxo fatty acid in Plantago ovata seed oil. Phytochem 26(11):3067–3069

    Article  CAS  Google Scholar 

  • Jansen RC, Stam P (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136:1447–1455

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jeffery AJ, Wilson V, Thein SL (1985) Hypervariable minisatellite regions in human DNA. Nature 314:67–72

    Article  Google Scholar 

  • Jones SD, Wipff JK, Montgomery PM (1997) Vascular plants of Texas: a comprehensive checklist including synonymy, bibliography, and index. University of Texas Press, Austin

    Google Scholar 

  • Josef P (2001) Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus lupulus L.) Euphytica 121:9–18

    Article  Google Scholar 

  • Joshi AK, Kumari M, Singh VP, Reddy CM, Kumar S, Rane J, Chand R (2007) Stay green trait: variation, inheritance and its association with spot blotch resistance in spring wheat (Triticum aestivum L.) Euphytica 153:59–71

    Article  Google Scholar 

  • Kaeppler SM, Phillips RL, Kim TS (1993) Use of near isogenic lines derived by backcrossing or selfing to map qualitative traits. Theor Appl Genet 87:233–237

    Article  CAS  PubMed  Google Scholar 

  • Karimzadeh G, Omidbaigi R (2004) Growth and seed characteristics of Isabgol (Plantago ovata Forsk) as influenced by some environmental factors. J Agric Sci Technol 6:103–110

    Google Scholar 

  • Kaswan V, Joshi A, Maloo SR (2013) Assessment of genetic diversity in Isabgol (Plantago ovata Forsk.) using 19 random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers for developing 20 crop improvement strategies. Afr J Biotechnol 12(23):3622–3635

    CAS  Google Scholar 

  • Khorana ML, Prabhu VG, Rao MRR (1958) Pharmacology of an alcoholic extract of Plantago ovata. Indian J Pharm 20:3–6

    CAS  Google Scholar 

  • Koller B, Lehmann A, McDermott JM, Gessler C (1993) Identification of apple cultivars using RAPD markers. Theor Appl Genet 85:901–904

    CAS  PubMed  Google Scholar 

  • Korbin M, Kuras A, Zurawicz E (2002) Fruit plant germplasm characterization using molecular markers generated in RAPD and ISSR-PCR. Cell Mol Biol Lett 7:785–794

    CAS  PubMed  Google Scholar 

  • Kotwal S, Dhar MK, Kour B et al (2013) Molecular markers unravel intraspecific and interspecific genetic variability in Plantago ovata and some of its wild allies. J Genet 92:293–298

    Article  PubMed  Google Scholar 

  • Kour B, Kotwal S, Dhar MK, Kaul S (2016) Genetic diversity analysis in Plantago ovata and some of its wild allies using RAPD markers. Russ Agric Sci 42(1):37–41

    Article  Google Scholar 

  • Kumar LD, Kathirvel M, Rao GV, Nagaraju J (2001) DNA profiling of disputed chilli samples (Capsicum annum) using ISSR-PCR and FISS-RPCR marker assays. Forensic Sci Int 116:63–68

    Article  CAS  PubMed  Google Scholar 

  • Kumar M, Fougat RS, Sharma AK et al (2014) Phenotypic and molecular characterization of selected species of Plantago with emphasis on Plantago ovata. Aust J Crop Sci 8(12):1639–1647

    Google Scholar 

  • Kumar S, Joshi UN, Saini ML et al (2015) Biomolecular characterization of guar [Cyamopsis tetragonoloba (L.)Taub.] genotypes along with wild species C. serrata and C. senegalensis. Plant Syst Evol 301(4):1249–1262

    Article  CAS  Google Scholar 

  • Kurata N, Nagamura Y, Miyao A et al (1994) Progress in cDNA analysis and construction of genetic and physical maps in RGP Presented in 4th International Workshop on Rice Molecular Biology 1–2 August, Science University of Tokyo, Tokyo, Japan

    Google Scholar 

  • Laidlaw RA, Percival EGV (1949) Studies on seed mucilage. Part III. Examination of polysaccharide extracted from the seeds of Plantago ovata Forsk. J Chem Soc:1600–1607

    Google Scholar 

  • Lata H, Chandra S, Techen N, Khan IA, ElSohly MA (2010) Assessment of the genetic stability of micropropagated plants of Cannabis sativa by ISSR markers. Planta Med 76:97–100

    Article  CAS  PubMed  Google Scholar 

  • Lee SK (1998) Identification of DNA polymorphisms in the field bean (Glycine soja S) using RAPD markers. J Korean Soc Grassland Sci 18:143–150

    Google Scholar 

  • Leroy XJ, Leon K, Branchard M (2000) Plant genomic instability detected by microsatellite-primers. Eur J Biotechnol 3(2):354–360

    Google Scholar 

  • Li P, Xiaoping Z, Yicun C et al (2008) Genetic diversity and germplasm resource research on tung tree (Vernicia fordii) cultivars, investigated by inter-simple sequence repeats. Afr J Biotechnol 7(8):1054–1059

    Google Scholar 

  • Lin XC, Lou YF, Liu J et al (2010) Crossbreeding of Phyllostachys species (Poaceae) and identification of their hybrids using ISSR markers. Genet Mol Res 9:1398–1404

    Article  CAS  PubMed  Google Scholar 

  • Marczewski W, Hennig J, Gebhardt C (2002) The potato virus S resistance gene Ns maps to potato chromosome VIII. Theor Appl Genet 105:564–567

    Article  CAS  PubMed  Google Scholar 

  • Meyer W, Mitchell TG, Freedman EZ, Vilgalys R (1993) Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans. J Clin Microbiol 31:2274–2280

    CAS  PubMed  PubMed Central  Google Scholar 

  • Mignouna HD, Ng NQ, Ikea J, Thottapilly G (1998) Genetic diversity in cowpea as revealed by random amplified polymorphic DNA. J Genet Breed 52:151–159

    CAS  Google Scholar 

  • Mithal BM, Gupta VD (1965) Suspending properties of Plantago ovata seed husk (Ispaghula) seed husk. Ind J Pharma 27(12):331–334

    Google Scholar 

  • Monna L, Miyao A, Inoue T et al (1994) Determination of RAPD markers in rice and their conversion into sequence tagged sites (STSs) and STS-specific primers. DNA Res 1:139–148

    Article  CAS  PubMed  Google Scholar 

  • Muchlbauer GJ, Staswick PE, Specht JE et al (1991) RFLP mapping using near isogenic lines in the soybean. Theor Appl Genet 81:189–198

    Article  Google Scholar 

  • Multani DS, Lyon BR (1995) Genetic fingerprinting of Australian cotton cultivars with RAPD markers. Genome 38:1005–1008

    Article  CAS  PubMed  Google Scholar 

  • Nagaraju J, Kathirvel M, Kumar RR et al (2002) Genetic analysis of traditional and evolved Basmati and non-Basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers. Proc Natl Acad Sci U S A 99:5836–5841

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Narzary D, Rana TS, Ranade SA (2010) Genetic diversity in inter-simple sequence repeat profiles across natural populations of Indian pomegranate (Punica granatum L.) Plant Biol 12:806–813

    Article  CAS  PubMed  Google Scholar 

  • Nkongolo KK (2003) Genetic characterization of Malawian cowpea (Vigna unguiculata L.) Walp landraces: diversity and gene flow among accessions. Euphytica 129:219–228

    Article  CAS  Google Scholar 

  • Orozco-Castillo C, Chalmers KJ, Waugh R, Powell W (1994) Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor Appl Genet 87:934–940

    Article  CAS  PubMed  Google Scholar 

  • Paredes M, Becerra V, Rojo C et al (2002) Ecotypic differentiation of Medicago polymorpha L. along an environmental gradient in central Chile. Euphytica 123:431–439

    Article  CAS  Google Scholar 

  • Paris HS, Yonash N, Portnoy V et al (2003) Assessment of genetic relationships in Cucurbita pepo (cucurbitaceae) using DNA markers. Theor Appl Genet 106:971–978

    Article  CAS  PubMed  Google Scholar 

  • Patel RB, Rana NG, Patel MR, Dhyani HK, Chauhan HV (1979) Chromatographic screening of proteins of Plantago ovata Forsk. Ind J Pharm Sci 41(6):249

    Google Scholar 

  • Paterson AH, de Verna JW, Lanini B, Tanksley SD (1990) Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecies cross of tomato. Genetics 124:735–742

    CAS  PubMed  PubMed Central  Google Scholar 

  • Punia A, Arora P, Yadav R, Chaudhury A (2009a) Optimization and inference of PCR conditions for genetic variability studies of commercially important cluster bean varieties by RAPD analysis. Asian Pac J Mol Biol Biotechnol 17(17):33–38

    Google Scholar 

  • Punia A, Yadav R, Arora P, Chaudhury A (2009b) Molecular and morphophysiological characterization of superior cluster bean (Cymopsis tetragonoloba) varieties. J Crop Sci Biotechnol 12(3):143–148

    Google Scholar 

  • Qiu YX, Fu CX, Wu FJ (2004) Analysis of population genetic structure and molecular identification of Changium smyrnioides and Chuanmin shenviolaceum with ISSR marker. Zhong Yao Cai 27:164–169

    Google Scholar 

  • Rajwade AV, Arora RS, Kadoo NY et al (2010) Relatedness of Indian flax genotypes (Linumu sitatissimum L.): an inter-simple sequence repeat (ISSR) primer assay. Mol Biotechnol 45:161–170

    Article  CAS  PubMed  Google Scholar 

  • Rani VK, Singh P, Shiran B et al (2000) Evidence for new nuclear and mitochondrial genome organizations among high frequency somatic embryogenesis derived plants of allotetraploid Coffea arabica L. (Rubiaceae). Plant Cell Rep 19:1013–1020

    Article  CAS  Google Scholar 

  • Ratnaparkhe MB, Gupta VS, Venmurthy MR, Ranjekar PK (1995) Genetic fingerprinting of pigeonpea [Cajanus cajan (L.) Mill sp.] and its wild relatives using RAPD markers. Theor Appl Genet 91:893–898

    Article  CAS  PubMed  Google Scholar 

  • Ravn H, Brimer L (1988) Structure and antibacterial activity of plantamajo side, a caffeic acid sugar ester from Plantago major subsp. major. Phytochemistry 27(11):3433–3437

    Article  CAS  Google Scholar 

  • Reunova GD, Kats IL, Muzarok TI, Zhuravlev IN (2010) Polymorphism of RAPD, ISSR and AFLP markers of the Panax ginseng CA Meyer (Araliaceae) genome. Genetika 46:1057–1066

    CAS  PubMed  Google Scholar 

  • Rohilla AK, Mukesh SA, Boora KS (2012) Genetic diversity analysis of the medicinal herb Plantago ovate (Forsk.) Afr J Biotechnol 11(92):15835–15842

    Article  CAS  Google Scholar 

  • Roy A, Bandyopadhyay AK, Mahapatra SK, Ghosh NK et al (2006) Evaluation of genetic diversity in jute (Corchorus species) using STMS, ISSR and RAPD markers. Plant Breed 125:292–297

    Article  CAS  Google Scholar 

  • Saiki RK, Scharf S, Faloona F et al (1985) Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnostics of sickle cell anaemia. Science 230:1350–1353

    Article  CAS  PubMed  Google Scholar 

  • Saiki RK, Gelfoud DH, Stoffel S et al (1988) Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491

    Article  CAS  PubMed  Google Scholar 

  • Samantaray S, Geetha KA, Hidayath KP, Maiti S (2010) Identification of RAPD markers linked to sex determination in guggal [Commiphora wightii (Arnott.)] Bhandari. Plant Biotechnol Rep 4:95

    Article  Google Scholar 

  • Sanchez MP, Davila JA, Loarce Y, Ferrer E (1996) Simple sequence repeat primers used in polymerase chain reaction amplifications to study genetic diversity in barley. Genome 39:112–117

    Article  Google Scholar 

  • Sandu SS, Hudson GJ, Kennedy JF (1981) The gel nature and structure of the carbohydrate of Ispaghula husk ex Plantago ovata Forsk. Carbohydr Res 93(2):247–259

    Article  Google Scholar 

  • Sankar AA, Moore GA (2001) Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of genetic linkage map. Theor Appl Genet 102:206–214

    Article  CAS  Google Scholar 

  • Santalla M, Power JB, Davey MR (1998) Genetic diversity in mungbean germplasm revealed by RAPD markers. Plant Breed 117:473–478

    Article  Google Scholar 

  • Sarwat M, Das S, Srivastava PS (2008) Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal Herb Plant. Cell Rep 27:519–528

    Article  CAS  Google Scholar 

  • Schierwater B, Ender A, Streit B (1993) The use of RAPD markers for molecular phylogenies. Verhdtzool Ges 86(1):58

    Google Scholar 

  • Senthil Kumar R, Parthiban KT, Govinda RM (2009) Molecular characterization of Jatropha genetic resources through inter-simple sequence repeat (ISSR) markers. Mol Biol Rep 36:951–956

    Article  CAS  Google Scholar 

  • Singh BM, Sharma KD, Katoch M, Guleria S, Sharma TR (2000) Molecular analysis of variability in Podophyllum hexandrum Royle an endangered medicinal herb of northwestern Himalaya. PGR Newslett 124:57–61

    Google Scholar 

  • Singh N, Lal RK, Shasany AK (2009) Phenotypic and RAPD diversity among 80 germplasm accessions of the medicinal plant isabgol (Plantago ovata, Plantaginaceae). Genet Mol Res 8(3):1273–1284

    Article  CAS  PubMed  Google Scholar 

  • Smith JSC, Smith DS (1992) Fingerprinting crop varieties. Adv Agron 47:85–140

    Article  CAS  Google Scholar 

  • Song Z, Li X, Wang H, Wang J (2010) Genetic diversity and population structure of Salvia miltiorrhizabge in China revealed by ISSR and SRAP. Genetica 138:241–249

    Article  CAS  PubMed  Google Scholar 

  • Souframanien J, Gopalakrishna T (2004) A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theor Appl Genet 109:687–1693

    Article  CAS  Google Scholar 

  • Souframanien J, Manjaya JG, Krishna TG, Pawar SE (2003) Random amplified polymorphic DNA analysis of cytoplasmic male sterile and male fertile pigeonpea (Cajanus cajan (L.) Millsp). Euphytica 129:293–299

    Article  CAS  Google Scholar 

  • Staub JE, Serquen FC, Gupta M (1996) Genetic markers, map construction and their application in plant breeding. Hortic Sci 31:729–740

    CAS  Google Scholar 

  • Steiner JJ, Piccioni E, Falcinelli M, Liston A (1998) Germplasm diversity among cultivars and the NPGS crimson clover collection. Crop Sci 38:263–271

    Article  Google Scholar 

  • Stiles JI, Lemme C, Sondur S, Morshid MB, Manshardt R (1993) Using randomly amplified polymorphic DNA for evaluating genetic relationship among papaya cultivars. Theor Appl Genet 85:697–701

    CAS  PubMed  Google Scholar 

  • Stuber CW, Lincoln SE, Wolf DW, Helentjaris T, Lander E (1992) Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics 132:823–839

    CAS  PubMed  PubMed Central  Google Scholar 

  • Tamhankar S, Ghate V, Raut A, Rajput B (2009) Molecular profiling of “Chirayat” using Inter Simple Sequence Repeat (ISSR) markers. Planta Med 75:1266–1270

    Article  CAS  PubMed  Google Scholar 

  • Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233

    Article  CAS  PubMed  Google Scholar 

  • Thimmappaiah WG, Santhosh D, Shobha GS, Melwyn (2009) Assessment of genetic diversity in cashew germplasm using RAPD and ISSR markers. Sci Hortic 120:411–417

    Article  CAS  Google Scholar 

  • Thomas KG, Bebeli PJ (2010) Genetic diversity of Greek Aegilops species using different types of nuclear genome markers. Mol Phylogenet Evol 56:951–961

    Article  CAS  PubMed  Google Scholar 

  • Thompson JA, Nelson RL, Vodkin LO (1998) Identification of diverse soybean germplasm using RAPD markers. Crop Sci 38:1362–1368

    Article  Google Scholar 

  • Tikunov YM, Khrustaleva LI, Karlov GI (2003) Application of ISSR markers in the genus, Lycopersicon. Euphytica 131:71–80

    Article  CAS  Google Scholar 

  • Tingey SV, del-Tufo JP (1993) Genetic analysis with random amplified polymorphic DNA markers. Plant Physiol 101:349–352

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tsumura Y, Ohba K, Strauss SH (1996) Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsugamenziesii) and sugi (Cryptomeria japonica). Theor Appl Genet 92:40–45

    Article  CAS  PubMed  Google Scholar 

  • Turley SD, Daggy BP, Dietschy JM (1994) Psyllium augments the cholesterol-lowering action of cholestyramine in hamsters by enhancing sterol loss from the liver. Gastroenterology 107(2):444–452

    Article  CAS  PubMed  Google Scholar 

  • Tyagi AP (1997) Investigation of quantitative trait loci (QTLs) in two diverse strains of pigeonpea (Cajanus cajan) using RAPD markers. J S Pac Agric 4:92–98

    Google Scholar 

  • Upadhyay KG, Patel AR, Vyas SH (1978) Evaluation of isabgul (psyllium) husk and gum acacia as ice cream stabilizers. Gujarat Agric Univ Res J 4:45–50

    Google Scholar 

  • Vahabi AA, Lotifi A, Solouki M, Bahrami S (2008) Molecular and morphological markers for the evaluation of diversity between Plantago ovata in Iran. Biotechnology 7(4):702–709

    Article  CAS  Google Scholar 

  • Vala AG, Fougat RS, Jadeja GC (2011) Genetic diversity of Plantago ovata Forsk through RAPD markers. Electronic J Plant Breed 2(4):592–596

    Google Scholar 

  • Verma PC, Chakrabarty D, Jena SN et al (2009) The extent of genetic diversity among Vanilla species: comparative results for RAPD and ISSR. Ind Crop Prod 29:581–589

    Article  CAS  Google Scholar 

  • Virk PS, Ford-Lloyd BV, Jackson MT, Nehbury HJ (1995) Use of RAPD for the study of diversity within plant germ collections. Heredity 74:170–179

    Article  CAS  PubMed  Google Scholar 

  • Wang G, Mahalingan R, Knap HT (1998) (C-A) and (GA) anchored simple sequence repeats (ASSRs) generated polymorphism in soybean, Glycine max (L.) Merr. Theor Appl Genet 96:1086–1096

    Article  CAS  Google Scholar 

  • Wang X, Zhao F, Hu Z et al (2008) Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic. Aquat Bot 88:154–159

    Article  CAS  Google Scholar 

  • Wasicky R (1961) Investigations of the seeds of Plantago ovata, P. psyllium and P. major variety cruneta as laxatives. Plant Med 9:232–244

    Article  CAS  Google Scholar 

  • Waugh R, Powell W (1992) Using RAPD markers for crop improvement. Trends Biotechnol 10:186–191

    Article  CAS  Google Scholar 

  • Waugh R, Baird E, Powell W (1992) The use of RAPD markers for the detection of gene introgression in potato. Plant Cell Rep 11:466–469

    Article  CAS  PubMed  Google Scholar 

  • Weber D, Helentjaris T (1989) Mapping RFLP loci in maize using B-A translocations. Genetics 2:583–590

    Google Scholar 

  • Weising K, Nybom H, Wolff K, Meyer W (1995) DNA fingerprinting in plants and fungi (ed.) CRC Press, Boca Raton, pp 1–3

    Google Scholar 

  • Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Williams TJ (1989) Plant germplasm preservation: a global perspective. In: Knutson L, Stoner AK (eds) Biotic diversity and germplasm preservation, global imperatives. Kluwer Academic Publishers, Amsterdam, pp 81–96

    Chapter  Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ et al (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu K, Jones R, Dannaeberger L, Scolnik PA (1994) Detection of microsatellite polymorphisms without cloning. Nucleic Acids Res 22:3257–3258

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu Z, Leng C, Tao Z, Wei Y, Jiang C (2009) Genetic diversity of Dioscorea alata based on ISSR analysis. Zhongguo Zhong Yao Za Zhi 34:3017–3020

    CAS  PubMed  Google Scholar 

  • Wu Y, Shi HM, Bao Z, Wang MY et al (2010) Application of molecular markers in predicting production quality of cultivated Cistanchede serticola. Biol Pharm Bull 33:334–339

    Article  CAS  PubMed  Google Scholar 

  • Xia T, Chen S, Chen S, Zhang D et al (2007) ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic Rhodiola chrysanthemifolia (Crassulaceae). Biochem Syst Ecol 35:209–214

    Article  CAS  Google Scholar 

  • Yadav R, Yadav N, Pal M, Goutam U (2014) Multiple shoot proliferation, bulblet induction and evaluation of genetic stability in Asiatic hybrid lily (Lilium sp.) Indian J Plant Physiol 18(4):354–359

    Article  Google Scholar 

  • Yadav R, Yadav N, Kumar S (2015) An improved micropropagation and assessment of genetic fidelity in multipurpose medicinal tree, Acacia auriculiformis. Proceedings of the National Academy of Sciences, India - Section B: Biological Sciences. doi:10.1007/s40011-015-0550-9

  • Yao HYZ, Chen DF, Chen JK, Zhou TS (2008) ISSR primer screening and preliminary evaluation of genetic diversity in wild populations of Glycyrrhiza uralensis. Biol Plant 52(1):117–120

    Article  CAS  Google Scholar 

  • Yeo AR, Yeo ME, Folowers SA, Flowers TJ (1990) Screening of rice (Oryza sativa L.) genotypes for physiological characters contributing to salinity resistance, and their relationship to overall performance. Theor Appl Genet 79:377–384

    Article  CAS  PubMed  Google Scholar 

  • Yuan XF, Dai ZH, Wang XD, Zhao B (2009) Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea involucrata by RAPD and ISSR markers. Biotechnol Lett 31:1279–1287

    Article  CAS  PubMed  Google Scholar 

  • Zhang ZY, Gali JY (1997) Utilization of RAPD in soybean germplasm resources and genetic linkage studies. Soybean Sci 16:60–65

    Google Scholar 

  • Zhang F, Lv Y, Dong H, Guo S (2010) Analysis of genetic stability through inter-simple sequence repeats molecular markers in micropropagated plantlets of Anoectochilus formosanus Hayata, a medicinal plant. Biol Pharm Bull 33:384–388

    Article  CAS  PubMed  Google Scholar 

  • Zheng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    Google Scholar 

  • Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeats (SSR)-anchored PCR amplification. Genomics 20:176–183

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Namita Singh .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Nature Singapore Pte Ltd.

About this chapter

Cite this chapter

Jaglan, S., Yadav, R., Siwach, P., Singh, N. (2017). Recent Updates on Molecular Biotechnological Intervention in Isabgol. In: Gahlawat, S., Salar, R., Siwach, P., Duhan, J., Kumar, S., Kaur, P. (eds) Plant Biotechnology: Recent Advancements and Developments. Springer, Singapore. https://doi.org/10.1007/978-981-10-4732-9_12

Download citation

Publish with us

Policies and ethics