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Molecular and Cellular Mechanisms of H+ Transport pp 447–454Cite as

Regulation of pHi in Oxyntic Cells in Intact Sheets of Frog Gastric Mucosa

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Part of the book series: NATO ASI Series ((volume 89))

Abstract

Gastric mucosal epithelial cells are regularly exposed to enormous concentrations of luminal H+, with gradients at times as high as 1,000,000:1. The ability of the mucosa to withstand such gradients has properly focused considerable attention on the mechanisms by which the mucosal epithelial cells regulate pHi. While several studies have previously examined the regulation of pHi in surface epithelial cells in intact amphibian antrum7,9,2,3,8,6 , or in monolayers of chief cells11, experiments to assess oxynticopeptic cells directly have been limited to isolated cells10,13,12 until recently. Since isolated cells invariably lose polarity, and because the interaction of the different types of cells within the complex fundic mucosa may be important,5 we have developed methods to measure pHi in intact amphibian fundic mucosa using the fluorescent dye.

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References

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Authors and Affiliations

  1. Department of Gastroenterology, Institute of Clinical Medicine University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305, Japan

    Akinora Yanaka M.D.

  2. Department of Surgery, Beth Israel Hospital, 330 Brookline Avenue, Boston, MA, 02215, USA

    William Silen M.D.

Authors
  1. Akinora Yanaka M.D.
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  2. William Silen M.D.
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Editor information

Editors and Affiliations

  1. Department of Physiological Sciences, University of Newcastle upon Tyne Medical School, Newcastle upon Tyne, NE2 4HH, UK

    Barry H. Hirst

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© 1994 Springer-Verlag Berlin Heidelberg

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Yanaka, A., Silen, W. (1994). Regulation of pHi in Oxyntic Cells in Intact Sheets of Frog Gastric Mucosa. In: Hirst, B.H. (eds) Molecular and Cellular Mechanisms of H+ Transport. NATO ASI Series, vol 89. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79301-1_51

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  • DOI: https://doi.org/10.1007/978-3-642-79301-1_51

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79303-5

  • Online ISBN: 978-3-642-79301-1

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