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In Vitro Method to Visualize UV-Induced Reactive Oxygen Species in a Skin Equivalent Model

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Textbook of Aging Skin

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Abstract

The skin is the only organ directly exposed to ultraviolet (UV) light from the sun. Oxidative cellular stress and DNA damage caused by UV exposure have been recognized to participate in various photogenesis of the skin. Among several oxidative stressors, reactive oxygen species (ROS) is well known to play important roles in the process of UV-induced skin damage including photoaging, immunomodulation, melanogenesis, and ultimately photo-carcinogenesis. To examine the impact of UV-induced ROS in the skin, it is critical to observe the ROS changes quantitatively in real time, while it has been challenging because ROS are extremely short-lived and essentially non-emissive. For this purpose, in the past two decades, several evaluation methods such as chemiluminescence, photoemission, fluorescence, or ESR spectroscopy using spin probes have been developed. With the advance of technologies, more and more methods became available not only to detect and quantify but also to visualize free radicals and ROS under much closer conditions to the actual human skin by utilizing the human skin equivalent models. These advanced in vitro visualization methods especially using human skin equivalent models enable us to more precisely characterize the ROS-related responses in human skin as a substitute for animal model and identify protective compounds against oxidative stress and its antiaging effect.

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Author information

Authors and Affiliations

  1. Beauty Technology Division, The Procter %26 Gamble Company, Cincinnati, OH, USA

    Tomohiro Hakozaki

  2. The Procter %26 Gamble Company, Mason Business Center, 8700 Mason Montgomery Road, Mason, OH, 45040, USA

    Tomohiro Hakozaki

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  1. Tomohiro Hakozaki
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Correspondence to Tomohiro Hakozaki .

Editor information

Editors and Affiliations

  1. Procter & Gamble Co., Cincinnati, Ohio, USA

    Miranda A. Farage

  2. Procter & Gamble Co., Cincinnati, Ohio, USA

    Kenneth W. Miller

  3. Department Dermatology, University of California, San Francisco School of Medicine, San Francisco, California, USA

    Howard I. Maibach

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© 2015 Springer-Verlag Berlin Heidelberg

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Hakozaki, T. (2015). In Vitro Method to Visualize UV-Induced Reactive Oxygen Species in a Skin Equivalent Model. In: Farage, M., Miller, K., Maibach, H. (eds) Textbook of Aging Skin. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27814-3_49-3

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  • DOI: https://doi.org/10.1007/978-3-642-27814-3_49-3

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Online ISBN: 978-3-642-27814-3

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Chapter history

  1. Latest

    In Vitro Method to Visualize UV-Induced Reactive Oxygen Species in a Skin Equivalent Model
    Published:
    11 November 2015

    DOI: https://doi.org/10.1007/978-3-642-27814-3_49-3

  2. Original

    In Vitro Method to Visualize UV-Induced Reactive Oxygen Species in a Skin Equivalent Model
    Published:
    28 July 2015

    DOI: https://doi.org/10.1007/978-3-642-27814-3_49-2

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