Abstract
Many biotechnological processes such as biogas production or defined biotransformations are carried out by microorganisms or tightly cooperating microbial communities. Process breakdown is the maximum credible accident for the operator. Any time savings that can be provided by suitable early-warning systems and allow for specific countermeasures are of great value. Process disturbance, frequently due to nutritional shortcomings, malfunction or operational deficits, is evidenced conventionally by process chemistry parameters. However, knowledge on systems microbiology and its function has essentially increased in the last two decades, and molecular biology tools, most of which are directed against nucleic acids, have been developed to analyze and diagnose the process. Some of these systems have been shown to indicate changes of the process status considerably earlier than the conventionally applied process chemistry parameters. This is reasonable because the triggering catalyst is determined, activity changes of the microbes that perform the reaction. These molecular biology tools have thus the potential to add to and improve the established process diagnosis system. This chapter is dealing with the actual state of the art of biogas process analysis in practice, and introduces molecular biology tools that have been shown to be of particular value in complementing the current systems of process monitoring and diagnosis, with emphasis on nucleic acid targeted molecular biology systems.
Keywords
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Abbreviations
- BB:
-
Bead-beating
- BMP:
-
Biological/biochemical methane potential
- BLAST:
-
Basic local alignment search tool
- Bp:
-
Base pair(s)
- cDNA:
-
Complementary DNA (transcribed from RNA species)
- CLSM:
-
Confocal laser scanning microscopy
- COD:
-
Chemical oxygen demand
- DGGE:
-
Denaturing-gradient gel electrophoresis
- DNA:
-
Deoxyribonucleic acid
- FISH:
-
Fluorescence in situ hybridization
- LCB:
-
Lignocellulosic biomass
- LM:
-
Light microscopy
- MQ:
-
Metabolic quotient
- mRNA:
-
Messenger RNA
- NA:
-
Nucleic acid(s)
- NGS:
-
Next generation sequencing
- OLR:
-
Organic loading rate
- PC(o)A:
-
Principal coordinate/Principal component analysis
- PCR:
-
Polymerase chain reaction
- PSM:
-
Process simulation model
- qPCR:
-
Quantitative Real-Time PCR
- rDNA:
-
Ribosomal deoxyribonucleic acid
- RNA:
-
Ribonucleic acid
- rRNA:
-
Ribosomal ribonucleic acid
- RT:
-
Reverse transcription
- SCFA:
-
Short-chain fatty acid(s) or also VFA
- SEM:
-
Scanning electron microscopy
- SMA:
-
Specific methanogenic activity
- TEM:
-
Transmission electron microscopy
- TGGE:
-
Temperature-gradient gel electrophoresis
- TVA/TIC:
-
Total volatile acids/total inorganic carbon
- VFA:
-
Volatile fatty acids
- VOA:
-
Volatile organic acids
References
Schink B (2006) Syntrophic associations in methanogenic degradation. In: Molecular basis of symbiosis, vol 41. Springer, Berlin, pp 1–19
McInerney MJ, Struchtemeyer CG, Sieber JR, Mouttaki H, Stams AJM, Schink B, Rohlin L, Gunsalus RP (2008) Physiology, ecology, phylogeny, and genomics of microorganisms capable of syntrophic metabolism. Ann N Y Acad Sci 1125:58–72
Demirel B, Scherer P (2008) The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review. Rev Environ Sci Biotechnol 7(2):173–190
Lebuhn M, Munk B, Effenberger M (2014) Agricultural biogas production in Germany—from practice to microbiology basics. Energy Sustain Soc 4(10):21
Thauer RK, Kaster A-K, Seedorf H, Buckel W, Hedderich R (2008) Methanogenic archaea: ecologically relevant differences in energy conservation. Nat Rev Microbiol 6(8):579–591
Stams AJM, Oude Elferink SJWH, Westermann P (2003) Metabolic interactions between methanogenic consortia and anaerobic respiring bacteria. Adv Biochem Eng Biotechnol 81:31–56
Fotidis IA, Karakashev D, Angelidaki I (2014) The dominant acetate degradation pathway/methanogenic composition in full-scale anaerobic digesters operating under different ammonia levels. Int J Environ Sci Technol 11(7):2087–2094
Schink B (1997) Energetics of syntrophic cooperation in methanogenic degradation. Microbiol Mol Biol Rev 61(2):262–280
Stams AJM, Plugge CM (2009) Electron transfer in syntrophic communities of anaerobic bacteria and archaea. Nat Rev Microbiol 7(8):568–577
McInerney MJ, Sieber JR, Gunsalus RP (2009) Syntrophy in anaerobic global carbon cycles. Curr Opin Biotechnol 20(6):623–632
Worm P, Koehorst JJ, Visser M, Sedano-Núñez VT, Schaap PJ, Plugge CM, Sousa DZ, Stams AJM (2014) A genomic view on syntrophic versus non-syntrophic lifestyle in anaerobic fatty acid degrading communities. Biochim Biophys Acta—Bioenerg 1837(12):2004–2016
Solli L, Håvelsrud OE, Horn SJ, Rike AG (2014) A metagenomic study of the microbial communities in four parallel biogas reactors. Biotechnol Biofuels 7(1):146
Kazda M, Langer S, Bengelsdorf FR (2014) Fungi open new possibilities for anaerobic fermentation of organic residues. Energy Sustain Soc 4(1):6
Koeck DE, Wibberg D, Maus I, Winkler A, Albersmeier A, Zverlov VV, Liebl W, Pühler A, Schwarz WH, Schlüter A (2014) Complete genome sequence of the cellulolytic thermophile Ruminoclostridium cellulosi wild-type strain {DG}5 isolated from a thermophilic biogas plant. J Biotechnol 188:136–137
Gruninger RJ, Puniya AK, Callaghan TM, Edwards JE, Youssef N, Dagar SS, Fliegerova K, Griffith GW, Forster R, Tsang A, McAllister T, Elshahed MS (2014) Anaerobic fungi (phylum Neocallimastigomycota): advances in understanding their taxonomy, life cycle, ecology, role and biotechnological potential. FEMS Microbiol Ecol 90(1):1–17
Weiland P (2010) Biogas production: current state and perspectives. Appl Microbiol Biotechnol 85(4):849–860
Nikolausz M, Walter RFH, Sträuber H, Liebetrau J, Schmidt T, Kleinsteuber S, Bratfisch F, Günther U, Richnow HH (2013) Evaluation of stable isotope fingerprinting techniques for the assessment of the predominant methanogenic pathways in anaerobic digesters. Appl Microbiol Biotechnol 97(5):2251–2262
Lv Z, Hu M, Harms H, Richnow HH, Liebetrau J, Nikolausz M (2014) Stable isotope composition of biogas allows early warning of complete process failure as a result of ammonia inhibition in anaerobic digesters. Bioresour Technol 167:251–259
Polag D, Krapf LC, Heuwinkel H, Laukenmann S, Lelieveld J, Keppler F (2014) Stable carbon isotopes of methane for real-time process monitoring in anaerobic digesters. Eng Life Sci 14(2):153–160
Van Soest PJ, McQueen RW (1973) The chemistry and estimation of fibre. Proc Nutr Soc 32(3):123–130
Krapf LC, Heuwinkel H, Schmidhalter U, Gronauer A (2013) The potential for online monitoring of short-term process dynamics in anaerobic digestion using near-infrared spectroscopy. Biomass Bioenergy 48:224–230
da Silva TL, Roseiro JC, Reis A (2012) Applications and perspectives of multi-parameter flow cytometry to microbial biofuels production processes. Trends Biotechnol 30(4):225–232
Rosselló-Móra R (2012) Towards a taxonomy of Bacteria and Archaea based on interactive and cumulative data repositories. Environ Microbiol 14:318–334
Stantscheff R, Kuever J, Rabenstein A, Seyfarth K, Dröge S, König H (2014) Isolation and differentiation of methanogenic Archaea from mesophilic corn-fed on-farm biogas plants with special emphasis on the genus Methanobacterium. Appl Microbiol Biotechnol 98(12):5719–5735
Musat N, Foster R, Vagner T, Adam B, Kuypers MMM (2012) Detecting metabolic activities in single cells, with emphasis on nanoSIMS. FEMS Microbiol Rev 36:486–511
Vanwonterghem I, Jensen PD, Ho DP, Batstone DJ, Tyson GW (2014) Linking microbial community structure, interactions and function in anaerobic digesters using new molecular techniques. Curr Opin Biotechnol 27:55–64
Voß E, Weichgrebe D, Rosenwinkel KH (2009) FOS/TAC: Herleitung, Methodik, Anwendung und Aussagekraft. In: Proceedings of the international scientific conference biogas science 2009, vol 3, pp 675–682
Quist-Wessel PMF, Langeveld J (2014) Bioenergy production from waste agricultural biomass. In: Langeveld H, Dixon J, Van Keulen H (eds) Biofuel cropping systems carbon, land and food. Taylor and Francis, Hoboken
Yenigün O, Demirel B (2013) Ammonia inhibition in anaerobic digestion: a review. Process Biochem 48(5–6):901–911
Hansen KH, Angelidaki I, Ahring BK (1998) Anaerobic digestion of swine manure: inhibition by ammonia. Water Res 32(1):5–12
Munk B, Bauer C, Gronauer A, Lebuhn M (2011) A metabolic quotient for methanogenic Archaea. Water Sci Technol 66(11):2311–2317
Munk B, Bauer C, Gronauer A, Lebuhn M (2010) Population dynamics of methanogens during acidification of biogas fermenters fed with maize silage. Eng Life Sci 10(6):496–508
Demirel B, Scherer P (2011) Trace element requirements of agricultural biogas digesters during biological conversion of renewable biomass to methane. Biomass Bioenergy 35(3):992–998
Vintiloiu A, Lemmer A, Oechsner H, Jungbluth T (2012) Mineral substances and macronutrients in the anaerobic conversion of biomass: an impact evaluation. Eng Life Sci 12(3):287–294
Chen Y, Cheng JJ, Creamer KS (2008) Inhibition of anaerobic digestion process: a review. Bioresour Technol 99(10):4044–4064
Mudhoo A, Kumar S (2013) Effects of heavy metals as stress factors on anaerobic digestion processes and biogas production from biomass. Int J Environ Sci Technol 10(6):1383–1398
Hecht C, Griehl C (2009) Investigation of the accumulation of aromatic compounds during biogas production from kitchen waste. Bioresour Technol 100(2):654–658
Sayder B, Vitz H, Mohring S, Merrettig-Bruns U, Kabasci S, Hamscher G, Tuerk J (2009) Gehemmte Biologie. Biogas-J 12(2):44–45
Young JC (2004) Respirometry for environmental science and engineering. SJ Enterprises, Springdale, Arkansas
Noike T, Endo G, Chang JE, Yaguchi J, Matsumoto J (1985) Characteristics of carbohydrate degradation and the rate-limiting step in anaerobic digestion. Biotechnol Bioeng 27(10):1482–1489
Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS (2002) Microbial cellulose utilization: fundamentals and biotechnology. Microbiol. Mol Biol Rev 66,(3):506–577
Merlin Christy P, Gopinath LR, Divya D (2014) A review on anaerobic decomposition and enhancement of biogas production through enzymes and microorganisms. Renew Sustain Energy Rev 34(C):167–173
Arantes V, Saddler JN (2010) Access to cellulose limits the efficiency of enzymatic hydrolysis: the role of amorphogenesis. Biotechnol Biofuels 3:4
Zhang Y-HP, Lynd LR (2005) Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysis. Biomacromolecules 6(3):1510–1515
Schwald W, Chart M, Breuil C, Saddler JN (1988) Applied microbiology biotechnology comparison of HPLC and colorimetric methods for measuring cellulolytic activity, pp 398–403
Khan AW, Tremblay D, LeDuy A (1986) Assay of xylanase and xylosidase activities in bacterial and fungal cultures. Enzyme Microb Technol 8(6):373–377
Obst U (1985) Test instructions for measuring the microbial metabolic activity in water samples. Fresenius’ Zeitschrift fuer Anal Chemie 321(2):166–168
Gasch C, Hildebrandt I, Rebbe F, Röske I (2013) Enzymatic monitoring and control of a two-phase batch digester leaching system with integrated anaerobic filter. Energy Sustain Soc 3(1):10
Mshandete AM, Björnsson L, Kivaisi AK, Steven M, Rubindamayugi T, Mattiasson B (2008) Two-stage anaerobic digestion of aerobic pre-treated sisal leaf decortications residues: hydrolases activities and biogas production profile. Afr J Biochem Res 2(5):211–218
Holzapfel-Pschorn A, Obst U, Haberer K (1987) Sensitive methods for the determination of microbial activities in water samples using fluorigenic substrates. Fresenius’ Zeitschrift fuer Anal Chemie 327(5–6):521–523
Regueiro L, Veiga P, Figueroa M, Alonso-Gutierrez J, Stams AJM, Lema JM, Carballa M (2012) Relationship between microbial activity and microbial community structure in six full-scale anaerobic digesters. Microbiol Res 167(10):581–589
Parawira W (2012) Enzyme research and applications in biotechnological intensification of biogas production. Crit Rev Biotechnol 32:172–186
Ahuja SK, Ferreira GM, Moreira AR (2004) Utilization of enzymes for environmental applications. Crit Rev Biotechnol 24(2–3):125–154
Warthmann R, Baier U, Meier U, Hersener J (2013) Optimisation of anaerobic digestion by pre-treatment, additives and process engineering. In: Biogas process optimisation workshop, 2013
Bruni E, Jensen AP, Angelidaki I (2010) Comparative study of mechanical, hydrothermal, chemical and enzymatic treatments of digested biofibers to improve biogas production. Bioresour Technol 101(22):8713–8717
Shen S, Nges IA, Yun J, Liu J (2014) Pre-treatments for enhanced biochemical methane potential of bamboo waste. Chem Eng J 240:253–259
Yu S, Zhang G, Li J, Zhao Z, Kang X (2013) Effect of endogenous hydrolytic enzymes pretreatment on the anaerobic digestion of sludge. Bioresour Technol 146:758–761
Diak J, Örmeci B, Kennedy KJ (2012) Effect of enzymes on anaerobic digestion of primary sludge and septic tank performance. Bioprocess Biosyst Eng 35(9):1577–1589
Plöchl M, Hilse A, Heiermann M, Suarez Quinones T, Budde J, Prochnow A (2009) Application of hydrolytic enzymes for improving biogas feedstock fluidity. CIGR E J IX:1–16
Burgess J, Pletschke B (2008) Hydrolytic enzymes in sewage sludge treatment: a mini-review. Water Sa 34(3):343–350
Davidsson Å, Wawrzynczyk O Norrlöw, Jansen J la C (2007) Strategies for enzyme dosing to enhance anaerobic digestion of sewage sludge. J Residuals Sci Technol 4(1):1–7
Vavilin VA, Rytov SV, Lokshina LY (1996) A description of hydrolysis kinetics in anaerobic degradation of particulate organic matter. Bioresour Technol 56(2–3):229–237
Gokhale A, Lu J, Lee I (2013) Immobilization of cellulase on magnetoresponsive graphene nano-supports. J Mol Catal B Enzym 90:76–86
Weiss S, Lebuhn M, Andrade D, Zankel A, Cardinale M, Birner-Gruenberger R, Somitsch W, Ueberbacher BJ, Guebitz GM (2013) Activated zeolite—Suitable carriers for microorganisms in anaerobic digestion processes? Appl Microbiol Biotechnol 97(7):3225–3238
Montalvo S, Guerrero L, Borja R, Sánchez E, Milán Z, Cortés I, Angeles de la la Rubia M (2012) Application of natural zeolites in anaerobic digestion processes: a review. Appl Clay Sci 58:125–133
Jeganathan J, Nakhla G, Bassi A (2007) Hydrolytic pretreatment of oily wastewater by immobilized lipase. J Hazard Mater 145(1–2):127–135
Kavitha S, Adish Kumar S, Yogalakshmi KN, Kaliappan S, Rajesh Banu J (2013) Effect of enzyme secreting bacterial pretreatment on enhancement of aerobic digestion potential of waste activated sludge interceded through EDTA. Bioresour Technol 150:210–219
Wang W, Yan L, Cui Z, Gao Y, Wang Y, Jing R (2011) Characterization of a microbial consortium capable of degrading lignocellulose. Bioresour Technol 102(19):9321–9324
Yan L, Gao Y, Wang Y, Liu Q, Sun Z, Fu B, Wen X, Cui Z, Wang W (2012) Diversity of a mesophilic lignocellulolytic microbial consortium which is useful for enhancement of biogas production. Bioresour Technol 111:49–54
Weiss S, Tauber M, Somitsch W, Meincke R, Müller H, Berg G, Guebitz GM (2010) Enhancement of biogas production by addition of hemicellulolytic bacteria immobilised on activated zeolite. Water Res 44(6):1970–1980
Kovács KL, Ács N, Kovács E, Wirth R, Rákhely G, Strang O, Herbel Z, Bagi Z (2013) Improvement of biogas production by bioaugmentation. Biomed Res Int 2013:482653
Bagi Z, Acs N, Bálint B, Horváth L, Dobó K, Perei KR, Rákhely G, Kovács KL (2007) Biotechnological intensification of biogas production. Appl Microbiol Biotechnol 76(2):473–482
Peng X, Börner RA, Nges IA, Liu J (2014) Impact of bioaugmentation on biochemical methane potential for wheat straw with addition of Clostridium cellulolyticum. Bioresour Technol 152:567–571
Levine SE, Fox JM, Blanch HW, Clark DS (2010) A mechanistic model of the enzymatic hydrolysis of cellulose. Biotechnol Bioeng 107(1):37–51
Christ O, Wilderer PA, Angerhöfer R, Faulstich M (2000) Mathematical modeling of the hydrolysis of anaerobic processes. Water Sci Technol 41(3):61–65
Rajendran K, Kankanala HR, Lundin M, Taherzadeh MJ (2014) A novel process simulation model (PSM) for anaerobic digestion using Aspen Plus. Bioresour Technol 168:7–13
Koch K, Lübken M, Gehring T, Wichern M, Horn H (2010) Biogas from grass silage—Measurements and modeling with ADM1. Bioresour Technol 101(21):8158–8165
Ramirez I, Mottet A, Carrère H, Déléris S, Vedrenne F, Steyer J-P (2009) Modified ADM1 disintegration/hydrolysis structures for modeling batch thermophilic anaerobic digestion of thermally pretreated waste activated sludge. Water Res 43(14):3479–3492
Batstone D (2001) The IWA anaerobic digestion model no 1, vol 1, no. 1, pp 1–68
Narihiro T, Kamagata Y (2013) Cultivating Yet-to-be Cultivated Microbes: The Challenge Continues. Microbes Environ 28(2):163–165
Hugenholtz P (2002) Exploring prokaryotic diversity in the genomic era. Genome Biol 3(2):0003.1–0003.8
Westerholm M, Levén L, Schnürer A (2012) Bioaugmentation of syntrophic acetate-oxidizing culture in biogas reactors exposed to increasing levels of ammonia. Appl Environ Microbiol 78(21):7619–7625
Munk B, Lebuhn M (2014) Process diagnosis using methanogenic Archaea in maize-fed, trace element depleted fermenters. Anaerobe 29:22–28
Metzker ML (2010) Sequencing technologies—the next generation. Nat Rev Genet 11(1):31–46
Fontaine M, Guillot E (2003) Study of 18S rRNA and rDNA stability by real-time RT-PCR in heat-inactivated Cryptosporidium parvum oocysts. FEMS Microbiol Lett 226(2):237–243
Keer JT, Birch L (2003) Molecular methods for the assessment of bacterial viability. J Microbiol Methods 53(2):175–183
Lebuhn M, Effenberger M, Gronauer A, Wilderer PA, Wuertz S (2003) Using quantitative real-time PCR to determine the hygienic status of cattle manure. Water Sci Technol 48(4):97–103
Garcés G (2005) Quantitative real-time PCR for detecting Cryptosporidium parvum in cattle manure and anaerobic digester samples—Methodological advances in {DNA} extraction. In: Proceedings of the 8th Latin American workshop and symposium on anaerobic digestion, pp 68–73
Garcés-Sanchez G, Wilderer PA, Munch JC, Horn H, Lebuhn M (2009) Evaluation of two methods for quantification of hsp70 mRNA from the waterborne pathogen Cryptosporidium parvum by reverse transcription real-time PCR in environmental samples. Water Res 43(10):2669–2678
Schmedes S, Marshall P, King JL, Budowle B (2013) Effective removal of co-purified inhibitors from extracted DNA samples using synchronous coefficient of drag alteration (SCODA) technology. Int J Legal Med 127(4):749–755
Lebuhn M, Effenberger M, Garcés G, Gronauer A, Wilderer PA (2004) Evaluating real-time PCR for the quantification of distinct pathogens and indicator organisms in environmental samples. Water Sci Technol 50(1):263–270
Dorak MT (ed) (2007) Real-time PCR, 1st edn. Taylor & Francis, New York
Lech M, Anders H-J (2014) Expression profiling by real-time quantitative polymerase chain reaction (RT-qPCR). Methods Mol Biol 1169:133–142
Kim J, Lim J, Lee C (2013) Quantitative real-time PCR approaches for microbial community studies in wastewater treatment systems: Applications and considerations. Biotechnol Adv 31(8):1358–1373
Navarro E, Serrano-Heras G, Castaño MJ, Solera J (2015) Real-time PCR detection chemistry. Clin Chim Acta 439:231–250
Terpe K (2013) Overview of thermostable DNA polymerases for classical PCR applications: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 97(24):10243–10254
McInerney P, Adams P, Hadi MZ (2014) Error rate comparison during polymerase chain reaction by DNA Polymerase. Mol Biol Int 2014:8
Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, Ciulla D, Tabbaa D, Highlander SK, Sodergren E, Methé B, DeSantis TZ (2011) Human microbiome consortium. In: F. Petrosino J, Knight R, Birren BW (eds) Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res 21(3):494–504
Sieber JR, McInerney MJ, Gunsalus RP (2012) Genomic insights into syntrophy: the paradigm for anaerobic metabolic cooperation. Annu Rev Microbiol 66(1):429–452
Bauer C, Korthals M, Gronauer A, Lebuhn M (2008) Methanogens in biogas production from renewable resources—a novel molecular population analysis approach. Water Sci Technol 58(7):1433–1439
Widmer G, Orbacz EA, Tzipori S (1999) beta-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability. Appl Environ Microbiol 65(4):1584–1588
McKillip JL, Jaykus LA, Drake M (1999) Nucleic acid persistence in heat-killed Escherichia coli O157:H7 from contaminated skim milk. J Food Prot 62(8):839–844
Ho DP, Jensen PD, Batstone DJ (2013) Methanosarcinaceae and acetate-oxidizing pathways dominate in high-rate thermophilic anaerobic digestion of waste-activated sludge. Appl Environ Microbiol 79(20):6491–6500
Nettmann E, Bergmann I, Pramschüfer S, Mundt K, Plogsties V, Herrmann C, Klocke M (2010) Polyphasic analyses of methanogenic archaeal communities in agricultural biogas plants. Appl Environ Microbiol 76(8):2540–2548
Li J, Rui J, Pei Z, Sun X, Zhang S, Yan Z, Wang Y, Liu X, Zheng T, Li X (2014) Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure. Appl Microbiol Biotechnol 98(10):4771–4780
Steinberg LM, Regan JM (2011) Response of lab-scale methanogenic reactors inoculated from different sources to organic loading rate shocks. Bioresour Technol 102:8790–8798
Zhang C, Yuan Q, Lu Y (2014) Inhibitory effects of ammonia on methanogen mcrA transcripts in anaerobic digester sludge. FEMS Microbiol Ecol 87(2):368–377
Lv Z, Leite AF, Harms H, Richnow HH, Liebetrau J, Nikolausz M (2014) Influences of the substrate feeding regime on methanogenic activity in biogas reactors approached by molecular and stable isotope methods. Anaerobe 29:91–99
Freitag TE, Prosser JI (2009) Correlation of methane production and functional gene transcriptional activity in a peat soil. Appl Environ Microbiol 75(21):6679–6687
Watanabe T, Kimura M, Asakawa S (2009) Distinct members of a stable methanogenic archaeal community transcribe mcrA genes under flooded and drained conditions in Japanese paddy field soil. Soil Biol Biochem 41(2):276–285
Amann RI, Ludwig W, Schleifer KH (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59(1):143–169
Talbot G, Topp E, Palin MF, Massé DI (2008) Evaluation of molecular methods used for establishing the interactions and functions of microorganisms in anaerobic bioreactors. Water Res 42(3):513–537
Rolfs A, Schuller I, Finckh U, Weber-Rolfs I (1992) Detection of single base changes using PCR. In: Rolfs A, Schuller I, Finckh U, Weber-Rolfs I (eds) PCR: clinical diagnostics and research. Springer, Berlin, pp 149–167
Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H (1986) Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol 51:263–273
Frigon D, Oerthe DB, Morgenroth E, Raskin L (2002) Oligonucleotide probe hybridization and modeling results suggest that populations consuming readily degradable substrate have high cellular RNA levels. Water Sci Technol 45(6):115–126
Candrian U (1995) Polymerase chain reaction in food microbiology. J Microbiol Methods 23(1):89–103
Roose-Amsaleg C, Garnier-Sillam E, Harry M (2001) Extraction and purification of microbial DNA from soil and sediment samples. Appl. Soil Ecol 18(1):47–60
Griffiths RI, Whiteley AS, O’Donnell AG, Bailey MJ (2000) Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition. Appl Environ Microbiol 66(12):5488–5491
Maukonen J, Mättö J, Wirtanen G, Raaska L, Mattila-Sandholm T, Saarela M (2003) Methodologies for the characterization of microbes in industrial environments: a review. J Ind Microbiol Biotechnol 30(6):327–356
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23(21):4407–4414
Janssen P, Bleeker M, Kersters K (1996) Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy, pp 1881–1893
Sklarz MY, Angel R, Gillor O, Soares MIM (2009) Evaluating amplified rDNA restriction analysis assay for identification of bacterial communities. Antonie Van Leeuwenhoek 96(4):659–664
Fisher MM, Triplett EW (1999) Automated approach for ribosomal intergenic spacer analysis of microbial diversity and its application to freshwater bacterial communities. Appl Environ Microbiol 65(10):4630–4636
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18(24):7213–7218
Power EG (1996) RAPD typing in microbiology—a technical review. J Hosp Infect 34(4):247–265
Versalovic J, Schneider M, de Bruijn FJ, Lupski JR (1994) Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5(25):25–40
Mills DK, Entry JA, Gillevet PM, Mathee K (2007) Assessing microbial community diversity using amplicon length heterogeneity polymerase chain reaction. Soil Sci Soc Am J 71(2):572
Schwartz DC, Cantor CR (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37(1):67–75
Liu WT, Marsh TL, Cheng H, Forney LJ (1997) Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ Microbiol 63(11):4516–4522
Nocker A, Burr M, Camper AK (2007) Genotypic microbial community profiling: a critical technical review. Microb Ecol 54(2):276–289
Fischer SG, Lerman LS (1983) DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proc Natl Acad Sci USA 80(6):1579–1583
Muyzer G, Smalla K (1998) “Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie van Leeuwenhoeck Int J Gen Mol Microbiol 73:127–141
Rosenbaum V, Riesner D (1987) Temperature-gradient gel electrophoresis. Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts. Biophys Chem 26(2–3):235–246
Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T (1989) Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci USA 86(8):2766–2770
Schwieger F, Tebbe CC (1998) A new approach to utilize PCR-single-strand-conformation polymorphism for 16S rRNA gene-based microbial community analysis. Appl Environ Microbiol 64(12):4870–4876
Su C, Lei L, Duan Y, Zhang K-Q, Yang J (2012) Culture-independent methods for studying environmental microorganisms: methods, application, and perspective. Appl Microbiol Biotechnol 93(3):993–1003
Wagner R (1994) The regulation of ribosomal RNA synthesis and bacterial cell growth. Arch Microbiol 161(2):100–109
Wintzingerode FV, Göbel UB, Stackebrandt E (2006) Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. FEMS Microbiol Rev 21(3):213–229
García-Martínez J, Acinas SG, Antón AI, Rodríguez-Valera F (1999) Use of the 16S-23S ribosomal genes spacer region in studies of prokaryotic diversity. J Microbiol Methods 36(1–2):55–64
Gürtler V, Stanisich A (1996) New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 142 (Pt 1):3–16
Schmid M, Schmitz-Esser S, Jetten M, Wagner M (2001) 16S-23S rDNA intergenic spacer and 23S rDNA of anaerobic ammonium-oxidizing bacteria: implications for phylogeny and in situ detection. Environ Microbiol 3(7):450–459
Ziganshina EE, Bagmanova AR, Khilyas IV, Ziganshin AM (2014) Assessment of a biogas-generating microbial community in a pilot-scale anaerobic reactor. J Biosci Bioeng 117(6):730–736
Mills DK, Fitzgerald K, Litchfield CD, Gillevet PM (2003) A comparison of DNA profiling techniques for monitoring nutrient impact on microbial community composition during bioremediation of petroleum-contaminated soils. J Microbiol Methods 54(1):57–74
Stahl DA, Capman WC (1994) Applications of molecular genetics to the study of microbial communities. NATO ASI Ser 35:193–206
Muyzer G (2000) Genetic fingerprinting of microbial communities—present status and future perspectives. In: Bell CR, Brylinski M, Johnson-Green P (eds) Microbial biosystems: new frontiers. proceedings of the 8th international symposium on microbial ecology. Atlantic Canada Society for Microbial Ecology, Halifax, pp 503–572
Collins G, Woods A, McHugh S, Carton MW, O’Flaherty V (2003) Microbial community structure and methanogenic activity during start-up of psychrophilic anaerobic digesters treating synthetic industrial wastewaters. FEMS Microbiol Ecol 46(2):159–170
Padmasiri SI, Zhang J, Fitch M, Norddahl B, Morgenroth E, Raskin L (2007) Methanogenic population dynamics and performance of an anaerobic membrane bioreactor (AnMBR) treating swine manure under high shear conditions. Water Res 41(1):134–144
McHugh S, Collins G, O’Flaherty V (2006) Long-term, high-rate anaerobic biological treatment of whey wastewaters at psychrophilic temperatures. Bioresour Technol 97(14):1669–1678
Scully C, Collins G, O’Flaherty V (2005) Assessment of anaerobic wastewater treatment failure using terminal restriction fragment length polymorphism analysis. J Appl Microbiol 99(6):1463–1471
Liesack W, Dunfield PF (2002) Biodiversity in soils: use of molecular methods for its characterization. In: Bitton G (ed) Encyclopedia of environmental microbiology. Wiley, New York, pp 4516–4522
Hugenholtz P, Goebel BM, Pace NR (1998) Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacteriol 180(18):4765–4774
Schmalenberger A, Tebbe CC (2014) Profiling the diversity of microbial communities with single-strand conformation polymorphism (SSCP). Methods Mol Biol 1096:71–83
Smalla K, Oros-Sichler M, Milling A, Heuer H, Baumgarte S, Becker R, Neuber G, Kropf S, Ulrich A, Tebbe CC (2007) Bacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of PCR-amplified 16S rRNA gene fragments: do the different methods provide similar results? J Microbiol Methods 69(3):470–479
Chachkhiani M, Dabert P, Abzianidze T, Partskhaladze G, Tsiklauri L, Dudauri T, Godon JJ (2004) 16S rDNA characterisation of bacterial and archaeal communities during start-up of anaerobic thermophilic digestion of cattle manure. Bioresour Technol 93(3):227–232
Werner JJ, Knights D, Garcia ML, Scalfone NB, Smith S, Yarasheski K, Cummings TA, Beers AR, Knight R, Angenent LT (2011) Bacterial community structures are unique and resilient in full-scale bioenergy systems. Proc Natl Acad Sci USA 108(10):4158–4163
Hill TCJ, Walsh KA, Harris JA, Moffett BF (2003) Using ecological diversity measures with bacterial communities. FEMS Microbiol Ecol 43(1):1–11
Yu D, Kurola JM, Kymäläinen M, Sinkkonen A, Romantschuk M (2014) Biogas production and methanogenic archaeal community in mesophilic and thermophilic anaerobic co-digestion processes. J Environ Manage 143:54–60
De Vrieze J, Verstraete W, Boon N (2013) Repeated pulse feeding induces functional stability in anaerobic digestion. Microb Biotechnol 6(4):414–424
Franke-Whittle IH, Goberna M, Pfister V, Insam H (2009) Design and development of the ANAEROCHIP microarray for investigation of methanogenic communities. J Microbiol Methods 79(3):279–288
Franke-Whittle IH, Knapp BA, Fuchs J, Kaufmann R, Insam H (2009) Application of COMPOCHIP microarray to investigate the bacterial communities of different composts. Microb Ecol 57(3):510–521
Goberna M, Gadermaier M, Schoen MA, Sperl D, Franke-Whittle IH, Wett B, Insam H (2012) Fingerprinting the microbial communities in organic wastes using oligonucleotide microarrays and Real-Time PCR. In: Trasar-Cepeda C, Hernandez T, Garcia C, Rad C, Gonzales-Carcedo S (eds) Soil enzymology in the recycling of organic wastes and environmental restoration, Burgos, Spain. Springer, Berlin, pp 285–298
Mutz K-O, Heilkenbrinker A, Lönne M, Walter J-G, Stahl F (2013) Transcriptome analysis using next-generation sequencing. Curr Opin Biotechnol 24(1):22–30
Carvalhais LC, Dennis PG, Tyson GW, Schenk PM (2012) Application of metatranscriptomics to soil environments. J Microbiol Methods 91(2):246–251
Solomon KV, Haitjema CH, Thompson DA, O’Malley MA (2014) Extracting data from the muck: deriving biological insight from complex microbial communities and non-model organisms with next generation sequencing. Curr Opin Biotechnol 28:103–110
Quail MA, Smith M, Coupland P, Otto TD, Harris SR, Connor TR, Bertoni A, Swerdlow HP, Gu Y (2012) A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genom 13(1):341
Segata N, Boernigen D, Tickle TL, Morgan XC, Garrett WS, Huttenhower C (2013) Computational meta’omics for microbial community studies. Mol. Syst. Biol. 9(1):666
Koren S, Phillippy AM (2015) One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly. Curr Opin Microbiol 23:110–120
Pinto AJ, Raskin L (2012) PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets. PLoS ONE 7(8):e43093
Lebuhn M, Hanreich A, Klocke M, Schlüter A, Bauer C, Pérez CM (2014) Towards molecular biomarkers for biogas production from lignocellulose-rich substrates. Anaerobe 29:10–21
Zakrzewski M, Goesmann A, Jaenicke S, Jünemann S, Eikmeyer F, Szczepanowski R, Al-Soud WA, Sørensen S, Pühler A, Schlüter A (2012) Profiling of the metabolically active community from a production-scale biogas plant by means of high-throughput metatranscriptome sequencing. J Biotechnol 158(4):248–258
Wagner M, Assmus B, Hartmann A, Hutzler P, Amann R (1994) In situ analysis of microbial consortia in activated sludge using fluorescently labelled, rRNA-targeted oligonucleotide probes and confocal scanning laser microscopy. J Microsc 176(3):181–187
Ploem JS (1967) The use of a vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incident light. Z Wiss Mikrosk 68:129–142
Caldwell DE, Korber DR, Lawrence JR (1992) Confocal laser microscopy and digital image analysis in microbial ecology. Adv Microb Ecol 12:1–67
Lawrence JR, Korber DR, Hoyle BD, Costerton JW, Caldwell DE (1991) Optical sectioning of microbial biofilms. J Bacteriol 173(20):6558–6567
Taylor DL, Salmon ED (1989) Basic fluorescence microscopy. In: Wang Y-L, Taylor DL (eds) Fluorescence microscopy of living cells in culture. Academic Press, San Diego, pp 207–237
Behnam F, Vilcinskas A, Wagner M, Stoecker K (2012) A straightforward DOPE (double labeling of oligonucleotide probes)-FISH (fluorescence in situ hybridization) method for simultaneous multicolor detection of six microbial populations. Appl Environ Microbiol 78(15):5138–5142
Amann R, Fuchs BM (2008) Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques. Nat Rev Microbiol 6(5):339–348
Amann R, Kühl M (1998) In situ methods for assessment of microorganisms and their activities. Curr Opin Microbiol 1:352–358
Raskin L, Poulsen LK, Noguera DR, Rittmann BE, Stahl DA (1994) Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization. Appl Environ Microbiol 60(4):1241–1248
Loy A, Horn M, Wagner M (2003) probeBase: an online resource for rRNA-targeted oligonucleotide probes. Nucleic Acids Res 31(1):514–516
Kepner RL, Pratt JR (1994) Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. Microbiol Rev 58(4):603–615
Sanz JL, Köchling T (2007) Molecular biology techniques used in wastewater treatment: An overview. Process Biochem 42(2):119–133
Diaper JP, Tither K, Edwards C (1992) Rapid assessment of bacterial viability by flow cytometry. Appl Microbiol Biotechnol 38(2)
Crosland-Taylor PJ (1953) A device for counting small particles suspended in a fluid through a Tube. Nature 171:37–38
Koch C, Harnisch F, Schröder U, Müller S (2014) Cytometric fingerprints: evaluation of new tools for analyzing microbial community dynamics. Front Microbiol 5:273
Wagner M, Ivleva NP, Haisch C, Niessner R, Horn H (2009) Combined use of confocal laser scanning microscopy (CLSM) and Raman microscopy (RM): investigations on EPS-Matrix. Water Res 43(1):63–76
Hoff K (1988) Rapid and simple method for double staining of bacteria with 4′,6-diamino-2-phenylindole and fluorescein isothiocyanate labelled antibodies. Appl Environ Microbiol 54(12):2949–2952
Lukinavičius G, Reymond L, D’Este E, Masharina A, Göttfert F, Ta H, Güther A, Fournier M, Rizzo S, Waldmann H, Blaukopf C, Sommer C, Gerlich DW, Arndt H-D, Hell SW, Johnsson K (2014) Fluorogenic probes for live-cell imaging of the cytoskeleton. Nat Methods 11(7):731–733
Grimm JB, Sung AJ, Legant WR, Hulamm P, Matlosz SM, Betzig E, Lavis LD (2013) Carbofluoresceins and carborhodamines as scaffolds for high-contrast fluorogenic probes. ACS Chem Biol 8(6):1303–1310
Hunter RC, Beveridge TJ (2005) Application of a pH-sensitive fluoroprobe (C-SNARF-4) for pH microenvironment analysis in Pseudomonas aeruginosa biofilms. Appl Environ Microbiol 71(5):2501–2510
Zebulun O (2013) Molecular and biological techniques used in landfill investigations: A mini-review. Biotechnol Mol Biol Rev 8(2):35–42
Lee N, Nielsen PH, Andreasen KH, Juretschko S, Nielsen JL, Schleifer KH, Wagner M (1999) Combination of fluorescent in situ hybridization and microautoradiography-a new tool for structure-function analyses in microbial ecology. Appl Environ Microbiol 65(3):1289–1297
Nielsen JL, Nielsen PH (2010) Combined microautoradiography and fluorescence in situ hybridization (MAR-FISH) for the identification of metabolically active microorganisms. In: Timmis KN (ed) Handbook of hydrocarbon and lipid microbiology. Springer, Berlin, pp 4093–4102
Collins G, Kavanagh S, McHugh S, Connaughton S, Kearney A, Rice O, Carrigg C, Scully C, Bhreathnach N, Mahony T, Madden P, Enright A-M, O’flaherty V (2006) Accessing the black box of microbial diversity and ecophysiology: recent advances through polyphasic experiments. J Environ Sci Health A Tox Hazard Subst Environ Eng 41(5):897–922
Okabe S, Kindaichi T, Ito T (2004) MAR-FISH—An ecophysiological approach to link phylogenetic affiliation and in situ metabolic activity of microorganisms at a single-cell resolution. Microbes Environ 19(2):83–98
Ariesyady HD, Ito T, Yoshiguchi K, Okabe S (2007) Phylogenetic and functional diversity of propionate-oxidizing bacteria in an anaerobic digester sludge. Appl Microbiol Biotechnol 75(3):673–683
Ariesyady HD, Ito T, Okabe S (2007) Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester. Water Res 41(7):1554–1568
Ito T, Yoshiguchi K, Ariesyady HD, Okabe S (2011) Identification of a novel acetate-utilizing bacterium belonging to Synergistes group 4 in anaerobic digester sludge. ISME J 5(12):1844–1856
Ito T, Yoshiguchi K, Ariesyady HD, Okabe S (2012) Identification and quantification of key microbial trophic groups of methanogenic glucose degradation in an anaerobic digester sludge. Bioresour Technol 123:599–607
Song H, Clarke WP, Blackall LL (2005) Concurrent microscopic observations and activity measurements of cellulose hydrolyzing and methanogenic populations during the batch anaerobic digestion of crystalline cellulose. Biotechnol Bioeng 91(3):369–378
Fernández N, Montalvo S, Fernández-Polanco F, Guerrero L, Cortés I, Borja R, Sánchez E, Travieso L (2007) Real evidence about zeolite as microorganisms immobilizer in anaerobic fluidized bed reactors. Process Biochem 42:721–728
Weiss S, Zankel A, Lebuhn M, Petrak S, Somitsch W, Guebitz GM (2011) Investigation of mircroorganisms colonising activated zeolites during anaerobic biogas production from grass silage. Bioresour Technol 102(6):4353–4359
Krakat N, Westphal A, Schmidt S, Scherer P (2010) Anaerobic digestion of renewable biomass: thermophilic temperature governs methanogen population dynamics. Appl Environ Microbiol 76(6):1842–1850
Cresson R, Dabert P, Bernet N (2009) Microbiology and performance of a methanogenic biofilm reactor during the start-up period. J Appl Microbiol 106(3):863–876
Karakashev D, Batstone DJ, Angelidaki I (2005) Influence of environmental conditions on methanogenic compositions in anaerobic biogas reactors. Appl Environ Microbiol 71(1):331–338
Crocetti G, Murto M, Björnsson L (2006) An update and optimisation of oligonucleotide probes targeting methanogenic Archaea for use in fluorescence in situ hybridisation (FISH). J Microbiol Methods 65:194–201
Hoshino T, Yilmaz LS, Noguera DR, Daims H, Wagner M (2008) Quantification of target molecules needed to detect microorganisms by fluorescence in situ hybridization (FISH) and catalyzed reporter deposition-FISH. Appl Environ Microbiol 74(16):5068–5077
Stoecker K, Dorninger C, Daims H, Wagner M (2010) Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) improves signal intensity and increases rRNA accessibility. Appl Environ Microbiol 76(3):922–926
Kumar RK, Chapple CC, Hunter N (1999) Improved double immunofluorescence for confocal laser scanning microscopy. J Histochem Cytochem 47:1213–1218
Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar Buchner A, Lai T, Steppi S, Jobb G, Förster WI, Brettske S, Gerber A, Ginhart W, Gross O, Grumann S, Hermann S, Jost R, König A, Liss T, Lüssmann R, May M, Nonhoff B, Reichel B, Strehlow R, Stamatakis A, Stuckmann N, Vilbig A, Lenke M, Ludwig T, Bode A, Schleifer K-H (2004) ARB: a software environment for sequence data. Nucleic Acids Res 32(4):1363–1371
Rotaru A-E, Shrestha PM, Liu F, Shrestha M, Shrestha D, Embree M, Zengler K, Wardman C, Nevin KP, Lovley DR (2014) A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane. Energy Environ Sci 7(1):408–415
Rotaru A-E, Shrestha PM, Liu F, Markovaite B, Chen S, Nevin K, Lovley D (2014) Direct interspecies electron transfer between Geobacter metallireducens and Methanosarcina barkeri. Appl Environ Microbiol 80(24):AEM.00895
Dubé CD, Guiot SR (2014) Direct interspecies electron transfer in granular sludge. In: International conference biogas science 2014, pp 85–86
Teng Z, Hua J, Wang C, Lu X (2014) Design and optimization principles of biogas reactors in large scale applications: research progress in lab—Immobilization. In: Shi F (ed) Reactor and process design in sustainable energy technology. Springer, Amsterdam, pp 125–127
Lalov IG, a. Krysteva M, Phelouzat JL (2001) Improvement of biogas production from vinasse via covalently immobilized methanogens. Bioresour Technol 79:83–85
Gong W, Liang H, Li W, Wang Z (2011) Selection and evaluation of biofilm carrier in anaerobic digestion treatment of cattle manure. Energy 36(5):3572–3578
Ahammad SZ, Davenport RJ, Read LF, Gomes J, Sreekrishnan TR, Dolfing J (2013) Rational immobilization of methanogens in high cell density bioreactors. RSC Adv 3:774
Bauer A, Theuretzbacher F, Rincón M, Enguidanos R (2014) Steam explosion pretreatment production of Alpine hay for enhancing biogas. In: Proceedings international conference of agricultural engineering, 2014, pp 4–7
Bharathiraja B, Sudharsanaa T, Bharghavi A, Sowmeya GS, Balaram G (2014) Insights on lignocellulosic pretreatments for biofuel production- SEM and reduction of lignin analysis, vol 6, no 9
Serrano-Lotina A, Daza L (2013) Highly stable and active catalyst for hydrogen production from biogas. J Power Sources 238:81–86
Knott G, Marchman H, Wall D, Lich B (2008) Serial section scanning electron microscopy of adult brain tissue using focused ion beam milling. J Neurosci 28(12):2959–2964
Ishitani T, Yaguchi T (1996) Cross-sectional sample preparation by focused ion beam: a review of ion-sample interaction. Microsc Res Tech 35(4):320–333
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Lebuhn, M., Weiß, S., Munk, B., Guebitz, G.M. (2015). Microbiology and Molecular Biology Tools for Biogas Process Analysis, Diagnosis and Control. In: Guebitz, G., Bauer, A., Bochmann, G., Gronauer, A., Weiss, S. (eds) Biogas Science and Technology. Advances in Biochemical Engineering/Biotechnology, vol 151. Springer, Cham. https://doi.org/10.1007/978-3-319-21993-6_1
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