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Vascularization for Tissue Engineering and Regenerative Medicine pp 1–14Cite as

Perfusion Bioreactors for Prevascularization Strategies in Cardiac Tissue Engineering

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Part of the book series: Reference Series in Biomedical Engineering ((TIENRE))

Abstract

Cardiac tissue engineering is currently being pursued with three different applications in mind: drug safety screening, disease modeling, and cardiac repair. Mini- and microengineered heart tissues are well suitable for drug safety screening and disease modeling. But generation of large cardiac patches of clinically relevant thickness, to functionally support the injured heart after myocardial infarction, still needs improvement. The high oxygen and nutrient demand request prevascularization of the engineered tissues in vitro prior to implantation. Vascularization and cardiac tissue development are influenced by several factors such as perfusion velocity, shear stress, coculture, extracellular matrix, mechanical strain, electrical stimulation, and many more. As engineering approaches get ever more sophisticated and bioreactors increasingly complex, cardiac tissue engineering evolves and quality control becomes more prominent. This chapter will focus on different perfusion bioreactors that aim at cultivating highly vascularized and functional engineered heart tissues by, e.g., direct perfusion through the tissue or cultivation on top of an engineered vascular bed.

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Author information

Authors and Affiliations

  1. Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany

    Ingra Mannhardt

  2. Cardiac Surgery and Engineering Group, Departments of Biomedicine and Surgery, University of Basel and University Hospital of Basel, Hebelstrasse 20, 4031, Basel, Switzerland

    Anna Marsano

  3. Department of Biochemical Engineering, University of Applied Sciences Technikum Wien, Höchstädtplatz 6, 1200, Wien, Austria

    Andreas Teuschl

Authors
  1. Ingra Mannhardt
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  2. Anna Marsano
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  3. Andreas Teuschl
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Corresponding author

Correspondence to Ingra Mannhardt .

Editor information

Editors and Affiliations

  1. AUVA Research Center, Ludwig Boltzmann Inst. Exp. a. Clinical AUVA Research Center, Wien, Holy See (Vatican City State)

    Wolfgang Holnthoner

  2. Department of Biomedicine, University Hospital Basel Department of Biomedicine, Basel, Switzerland

    Andrea Banfi

  3. Institute for Pathology, Johannes Gutenberg University Institute for Pathology, Mainz, Germany

    James Kirkpatrick

  4. LBI für experimentelle und klinisch , Wien, Austria

    Heinz Redl

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Mannhardt, I., Marsano, A., Teuschl, A. (2017). Perfusion Bioreactors for Prevascularization Strategies in Cardiac Tissue Engineering. In: Holnthoner, W., Banfi, A., Kirkpatrick, J., Redl, H. (eds) Vascularization for Tissue Engineering and Regenerative Medicine. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-21056-8_14-1

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  • DOI: https://doi.org/10.1007/978-3-319-21056-8_14-1

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-21056-8

  • Online ISBN: 978-3-319-21056-8

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