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Quantitative Analysis with Droplet Digital PCR

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Phytoplasmas

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1875))

Abstract

Digital PCR-based methods, such as droplet digital PCR, are one of the best tools for determination of absolute nucleic-acid copy numbers. These techniques avoid the need for reference materials with known target concentrations. Compared to real-time PCR, they provide higher accuracy of quantification at low target concentrations, and have higher resilience to inhibitors. In this Chapter, we describe the droplet digital PCR workflow for the detection and quantification of flavescence dorée phytoplasma.

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Acknowledgment

This work was supported by the Slovenian Research Agency (grant number P4-0165) and by Euphresco Project 2016-A-215, financed by the Ministry of Agriculture, Forestry and Food through the Administration of the Republic of Slovenia for Food Safety, Veterinary and Plant Protection. The work was performed using droplet dPCR equipment financed by the Metrology Institute of the Republic of Slovenia (MIRS), with financial support from the European Regional Development Fund. The equipment is wholly owned by the Republic of Slovenia.

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Authors and Affiliations

  1. National Institute of Biology, Ljubljana, Slovenia

    Nataša Mehle & Tanja Dreo

Authors
  1. Nataša Mehle
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  2. Tanja Dreo
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Corresponding author

Correspondence to Nataša Mehle .

Editor information

Editors and Affiliations

  1. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Rita Musetti

  2. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Laura Pagliari

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Mehle, N., Dreo, T. (2019). Quantitative Analysis with Droplet Digital PCR. In: Musetti, R., Pagliari, L. (eds) Phytoplasmas. Methods in Molecular Biology, vol 1875. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8837-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8837-2_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8836-5

  • Online ISBN: 978-1-4939-8837-2

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