Abstract
The middle cerebral artery occlusion (MCAO) model is widely used for inducing a focal cerebral ischemic insult (stroke) in rodents. Here, we describe a method for transient MCAO technique without craniotomy in both mice and rats. In our laboratory, this technique yields consistent secondary brain damage that evolves over a period of 3–7 days of reperfusion after transient MCAO. We also describe the methods for analyzing postischemic motor dysfunction and infarct volume in rodents subjected to transient MCAO.
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Lopez, M.S., Vemuganti, R. (2018). Modeling Transient Focal Ischemic Stroke in Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion. In: Tharakan, B. (eds) Traumatic and Ischemic Injury. Methods in Molecular Biology, vol 1717. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7526-6_9
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DOI: https://doi.org/10.1007/978-1-4939-7526-6_9
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