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Extracellular Vesicles pp 91–103Cite as

Polymer-Based Purification of Extracellular Vesicles

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1660))

Abstract

Lipid particles, including exosomes (extracellular vesicles [EVs]), are released from cells in vivo and in vitro. The contents of these EVs can be indicative of disease and therefore be utilized in diagnostic biophysical or biochemical assays. To make use of EVs in this way, methods for purification and quantification are required which vary depending on their particles origin and concentration. This chapter provides an overview of EV purification techniques and provides detailed instructions on the purification of EVs by polymer based precipitation and subsequent quantification. The subsequent quantification and characterization of these EVs also presents a challenge, as limited methods are capable of detecting EVs due to their small size.

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Acknowledgment

We thank the National Institutes of Health and Cancer Research UK for financial supports (NIH R01GM103843 and C596/A17096).

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Authors and Affiliations

  1. Drug Discovery Programme, Beatson Institute for Cancer Research, Bearsden, Glasgow, G61 1BD, UK

    Peter N. Brown

  2. Department of Chemistry & Biochemistry, University of Colorado Boulder, 3415 Colorado Ave, Boulder, CO, 80309, USA

    Hang Yin

  3. BioFrontiers Institute, University of Colorado Boulder, 3415 Colorado Ave, Boulder, CO, 80309, USA

    Hang Yin

Authors
  1. Peter N. Brown
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  2. Hang Yin
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Corresponding author

Correspondence to Hang Yin .

Editor information

Editors and Affiliations

  1. CloudHealth Genomics, Ltd., Shanghai, China

    Winston Patrick Kuo

  2. Predicine, Inc., Hayward, California, USA

    Shidong Jia

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Brown, P.N., Yin, H. (2017). Polymer-Based Purification of Extracellular Vesicles. In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7253-1_8

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7251-7

  • Online ISBN: 978-1-4939-7253-1

  • eBook Packages: Springer Protocols

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