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Isolation and Culture of Adipose-Derived Stromal Cells from Subcutaneous Fat

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Book cover Fibrosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1627))

Abstract

Cells with mesenchymal stem cell characteristics can be isolated from human adipose tissue stroma. Relative ease of isolation in large numbers and their ability for expansion and differentiation means that they are becoming a preferred cell type for mesenchymal regenerative medicine applications. In addition to expansion and differentiation capacity, they also express valuable paracrine activities which promote tissue formation and wound healing, including pro- and anti-fibrotic mediators. Here we describe a method for routine isolation of adipose stromal cells, culture expansion, and characterization by differentiation and then production of conditioned media.

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Authors and Affiliations

  1. Ear Science Institute Australia, Perth, Western Australia, Australia

    Lawrence J. Liew, Huan Ting Ong & Rodney J. Dilley

  2. School of Surgery (M509), University of Western Australia, Perth, Western Australia, Australia

    Lawrence J. Liew & Rodney J. Dilley

  3. Center for Cell Therapy and Regenerative Medicine, University of Western Australia, Perth, Western Australia, Australia

    Rodney J. Dilley

Authors
  1. Lawrence J. Liew
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  2. Huan Ting Ong
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  3. Rodney J. Dilley
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Corresponding author

Correspondence to Rodney J. Dilley .

Editor information

Editors and Affiliations

  1. Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, USA

    Laure Rittié

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Liew, L.J., Ong, H.T., Dilley, R.J. (2017). Isolation and Culture of Adipose-Derived Stromal Cells from Subcutaneous Fat. In: Rittié, L. (eds) Fibrosis. Methods in Molecular Biology, vol 1627. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7113-8_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7113-8_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7112-1

  • Online ISBN: 978-1-4939-7113-8

  • eBook Packages: Springer Protocols

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