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Quantitative Real-time Polymerase Chain Reaction

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Quantitative real-time polymerase chain reaction, or qRT-PCR for short, is a commonly used laboratory technique in which a particular DNA molecule is amplified and detected in real time as the reaction is occurring. When used concomitantly with reverse transcription, qRT-PCR provides a highly sensitive means for quantifying gene expression in the form of messenger RNA (mRNA).

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Correspondence to Steven D. Rhodes .

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© 2013 Springer Science+Business Media, LLC

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Rhodes, S.D. (2013). Quantitative Real-time Polymerase Chain Reaction. In: Dubitzky, W., Wolkenhauer, O., Cho, KH., Yokota, H. (eds) Encyclopedia of Systems Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-9863-7_884

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