Abstract
Myeloid-derived suppressor cells (MDSC) are known to inhibit functions of T and NK cells. MDSC have been shown to be generated and to accumulate under chronic inflammatory conditions that are typical for cancer. Therefore, it would be highly beneficial to find ways to diminish the number and immunosuppressive functions of these cells in tumor-bearing hosts. Here we describe current protocols to deplete MDSC or induce their maturation in preclinical tumor models that could lead to the attenuation of their immunosuppressive functions.
Key words
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Gabrilovich DI (2017) Myeloid-derived suppressor cells. Cancer Immunol Res 5:3–8
Medina E, Hartl D (2018) Myeloid-derived suppressor cells in infection: a general overview. J Innate Immun 255:210–221
Bronte V, Brandau S, Chen S-H, Colombo MP, Frey AB, Greten TF et al (2016) Recommendations for myeloid-derived suppressor cell nomenclature and characterization standards. Nat Commun 7:12150
Movahedi K, Guilliams M, Van den Bossche J, Van den Bergh R, Gysemans C, Beschin A et al (2008) Identification of discrete tumor-induced myeloid-derived suppressor cell subpopulations with distinct T cell suppressive activity. Blood 111:4233–4244
Zhou J, Nefedova Y, Lei A, Gabrilovich D (2018) Neutrophils and PMN-MDSC: their biological role and interaction with stromal cells. Semin Immunol 35:19–28
Vincent J, Mignot G, Chalmin F, Ladoire S, Bruchard M, Chevriaux A et al (2010) 5-fluorouracil selectively kills tumor-associated myeloid-derived suppressor cells resulting in enhanced T cell-dependent antitumor immunity. Cancer Res 70:3052–3061
Draghiciu O, Nijman HW, Hoogeboom BN, Meijerhof T, Daemen T (2015) Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication. Onco Targets Ther 4:e989
Sevko A, Michels T, Vrohlings M, Umansky L, Beckhove P, Kato M et al (2013) Antitumor effect of paclitaxel is mediated by inhibition of myeloid-derived suppressor cells and chronic inflammation in the spontaneous melanoma model. J Immunol 190:2464–2471
Daley JM, Thomay AA, Connolly MD, Reichner JS, Albina JE (2008) Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol 83:64–70
Kuwata T, Wang IM, Tamura T, Ponnamperuma RM, Levine R, Holmes KL et al (2000) Vitamin a deficiency in mice causes a systemic expansion of myeloid cells. Blood 95:3349–3356
Kusmartsev S, Cheng F, Yu B, Nefedova Y, Sotomayor E, Lush R et al (2003) All-trans-retinoic acid eliminates immature myeloid cells from tumor-bearing mice and improves the effect of vaccination. Cancer Res 66:9299–9307
Nefedova Y, Fishman M, Sherman S, Wang X, Beg AA, Gabrilovich DI (2007) Mechanism of all-trans retinoic acid effect on tumor-associated myeloid-derived suppressor cells. Cancer Res 67:11021–11028
Kusmartsev S, Gabrilovich DI (2003) Inhibition of myeloid cell differentiation in cancer: the role of reactive oxygen species. J Leukoc Biol 74:186–196
Cheng P, Corzo CA, Luetteke N, Yu B, Nagaraj S, Bui MM et al (2008) Inhibition of dendritic cell differentiation and accumulation of myeloid-derived suppressor cells in cancer is regulated by S100A9 protein. J Exp Med 205:2235–2249
Donyai P, Sewell GJ (2006) Physical and chemical stability of paclitaxel infusions in different container types. J Oncol Pharm Pract 12:211–222
Geary SM, Lemke CD, Lubaroff DM, Salem AK (2013) The combination of a low-dose chemotherapeutic agent, 5-fluorouracil, and an adenoviral tumor vaccine has a synergistic benefit on survival in a tumor model system. PLoS One 8:e67904
Srivastava MK, Zhu L, Harris-White M, Kar U, Huang M, Johnson MF et al (2012) Myeloid suppressor cell depletion augments antitumor activity in lung cancer. PLoS One 7:e40677
Hurez V, Daniel BJ, Sun L, Liu AJ, Ludwig SM, Kious MJ et al (2012) Mitigating age-related immune dysfunction heightens the efficacy of tumor immunotherapy in aged mice. Cancer Res 15:2089–2099
Kumar V, Cheng P, Condamine T, Mony S, Languino LR, McCaffrey JC et al (2016) CD45 phosphatase inhibits STAT3 transcription factor activity in myeloid cells and promotes tumor-associated macrophage differentiation. Immunity 44:303–315
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Groth, C., Weber, R., Utikal, J., Umansky, V. (2021). Depletion and Maturation of Myeloid-Derived Suppressor Cells in Murine Cancer Models. In: Brandau, S., Dorhoi, A. (eds) Myeloid-Derived Suppressor Cells. Methods in Molecular Biology, vol 2236. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1060-2_7
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1060-2_7
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1059-6
Online ISBN: 978-1-0716-1060-2
eBook Packages: Springer Protocols