Abstract
Plants take up inorganic nutrients from the soil by transport proteins located in the plasma membrane of root cells. Boron (B) is an essential element for plant growth; it taken up and translocated by boric acid channels such as NIP5;1 and borate exporters such as BOR1 in Arabidopsis. NIP5;1 and BOR1 are localized to the plasma membrane of various root cells in polar manners toward soil- and stele-side, respectively, for efficient transport of B. In response to elevated B concentration, BOR1 undergoes vacuolar sorting for degradation to avoid accumulation of B to a toxic level in tissues. The polar localization and vacuolar sorting of the transport proteins are regulated through differential mechanisms of endocytosis and intracellular trafficking. In this chapter, we describe methods for quantitative live-cell imaging of GFP-NIP5;1 and BOR1-GFP as markers for the polar and vacuolar trafficking.
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Acknowledgments
We thank Chinami Shinohara (Osaka Prefecture University) for providing a confocal micrograph of GFP-NIP5;1. This research was, in part, supported by Grant-in-Aid for Young Scientists (19K16164) to Akira Yoshinari and Grants-in-Aid (26712007 and 19H00934) to Junpei Takano from the Japan Society for the Promotion of Science.
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Yoshinari, A., Takano, J. (2020). Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis. In: Otegui, M. (eds) Plant Endosomes. Methods in Molecular Biology, vol 2177. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0767-1_1
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DOI: https://doi.org/10.1007/978-1-0716-0767-1_1
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