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Virus-Induced Gene Silencing in Plants pp 139–154Cite as

Virus-Induced Gene Silencing for Functional Genomics in Withania somnifera, an Important Indian Medicinal Plant

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2172))

Abstract

Virus-induced gene silencing (VIGS) has emerged as a fast and efficient reverse and forward genetics tool to study gene function in model plants as well as in agriculturally important plants. In addition, VIGS approach has been successfully used to provide insights into the role of several genes and regulators involved in plant secondary metabolism. Ashwagandha (Withania somnifera) is an important Indian medicinal plant that accumulates pharmacologically important triterpenoid steroidal lactones, which are collectively termed as withanolides. W. somnifera being a highly recalcitrant plant for genetic transformation, Tobacco rattle virus (TRV)-mediated VIGS was established by our group to facilitate understanding of withanolides’ pathway. Here, we describe a detailed procedure to carry out VIGS for gene function studies in W. somnifera.

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Acknowledgments

This work was supported by the GAP376 project (SERB funded project EMR/2016/002746) of CSIR-Central Institute of Medicinal and Aromatic Plants. D.P.B., H.B.S., and S.R.K. are the recipients of the Research Fellowships from the University Grants Commission (UGC), Indian Council of Medical Research (ICMR), and Council of Scientific and Industrial Research (CSIR), respectively. The institutional communication number for this article is CIMAP/PUB/2019/May/40.

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Author notes

  1. Dikki Pedenla Bomzan and H. B. Shilpashree contributed equally.

Authors and Affiliations

  1. Molecular Plant Biology and Biotechnology Lab, CSIR-Central Institute of Medicinal and Aromatic Plants Research Centre, Bengaluru, India

    Dikki Pedenla Bomzan, H. B. Shilpashree, P. Anjali, Sarma Rajeev Kumar & Dinesh A. Nagegowda

Authors
  1. Dikki Pedenla Bomzan
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  2. H. B. Shilpashree
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  3. P. Anjali
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  4. Sarma Rajeev Kumar
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  5. Dinesh A. Nagegowda
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Corresponding author

Correspondence to Dinesh A. Nagegowda .

Editor information

Editors and Affiliations

  1. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Vincent Courdavault

  2. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Sébastien Besseau

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Bomzan, D.P., Shilpashree, H.B., Anjali, P., Kumar, S.R., Nagegowda, D.A. (2020). Virus-Induced Gene Silencing for Functional Genomics in Withania somnifera, an Important Indian Medicinal Plant. In: Courdavault, V., Besseau, S. (eds) Virus-Induced Gene Silencing in Plants. Methods in Molecular Biology, vol 2172. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0751-0_11

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  • DOI: https://doi.org/10.1007/978-1-0716-0751-0_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0750-3

  • Online ISBN: 978-1-0716-0751-0

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