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In vitro Conservation Technique for Russian Citrus limon

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Abstract

In vitro storage conditions of Citrus limon (L.) Burm. cv. Novoafonsky were developed in this study. Three explants were used: nodal regenerants, seedlings, and micrografted plants. The results obtained in present investigation confirmed that slow growth maintenance of lemon microplants on ½ MS medium allows to store micro-grafted plants and seedlings for 8 and 12 months, respectively, without subculturing. It was also noted that low temperature +10 ± 2 °C and light intensity of 1,000 lux maintain seedlings till 12 months and micrografted plants till 8 months on ½ MS medium. The decrease of chlorophyll and carotenoids in leaves was observed during the storage of microplants. It was observed that low temperature and low light intensity were better for the preservation of the photosynthetic apparatus and accumulation of photosynthates in the leaves of lemon regenerants, thereby maintaining a high level of their viability. Thus, the best treatment for in vitro conservation was micrografted plants on ½ MS media in temperature +10 ± 2 °C and light intensity of 1,000 lux.

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References

  1. Avenido RA, Endonela LE, Pateña LF, Barba RC (2005) Developing plant regeneration systems for in vitro conservation of mandarin (C. reticulata) and pummel (C. maxima). ISHS Acta Horticulturae, 694: International Symposium on Harnessing the Potential of Horticulture in the Asian-Pacific Region

  2. Barlass M, Skene KGM (1982) In vitro plantlet formation from Citrus species and hybrids. Sci Hort 17:333–341

    Article  Google Scholar 

  3. Benabdesselam FM, Benabdesselam M, Khettal B, Bedjou F (2011) Micropropagation of Algerian juvenile rootstocks Citrus species. Life Sciences Leaflets 18:707–717

    Google Scholar 

  4. Budagovski A, Budagovski O, Budagovski I (2010) Paradoxes of the optical properties of the green cells and their practical application. Photonics. http://www.photonics.su/journal/article/2537

  5. Chaturvedi HC, Singh SK, Sharma AK (2002) Method for regeneration viable and fertile citrus plants by tissue culture from explants. United States Patent 6:485–975

    Google Scholar 

  6. Chen XD (2012) Germplasm conservation and microRNA identification of in vitro plantlets in Citrus trees (Thesis). http://www.globethesis.com

  7. Fogel VA (2008) Directory of Citrus collection of Research Institute of Floriculture and Subtropical Crops/ed. by A.V. Ryndin––Russia, Sochi, p 55

  8. Jajoo A (2010) In vitro propagation of Citrus limonia Osbeck through nucellar embryo culture. Curr Res J Bio Sci 2(1):6–8

    CAS  Google Scholar 

  9. Jinren Z, Shanchun C, Yin Q, Zhusheng C, Tianchi G (2010) Studies on germplasm conservation in vitro of plantlets and the changes of endogenous hormones in the process of conservation in citrus plants. http://mt.china-papers.com/1/?p=160162

  10. Khan IA (2007) Citrus genetics, breeding and biotechnology– CAB International, 370

  11. Marin ML, Duran-Vila N (1991) Conservation of citrus germplasm in vitro. Am Soc Hort Sci 116:740–746

    Google Scholar 

  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plants 15:473–497

    Article  CAS  Google Scholar 

  13. Shlyk A (1972) The synthesis of chlorophyll and the formation of photosynthetic systems. Theoretical Foundations of Photosynthetic Productivity, Moscow

    Google Scholar 

  14. Veselova TV, Vysotskaya ON, Veselovsky VA (1994) Assessment of the status of plants, strawberries, cultivated in vitro by fluorescent method. Physiol Plants 41(6):942–946

    Google Scholar 

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Authors and Affiliations

  1. The State Research Institution All–Russian Scientific and Research Institute of Floriculture and Subtropical Crops of the Russian Academy of Agricultural Sciences, Sochi, Russia

    L. S. Samarina, T. M. Kolomiets & Y. S. Abilfazova

  2. Indian Agricultural Research Institute, Regional Station, Pusa, Samastipur, 848 125, Bihar, India

    Ravish Choudhary & P. L. Saran

Authors
  1. L. S. Samarina
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  2. Ravish Choudhary
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  3. T. M. Kolomiets
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  4. Y. S. Abilfazova
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  5. P. L. Saran
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Correspondence to Ravish Choudhary.

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Samarina, L.S., Choudhary, R., Kolomiets, T.M. et al. In vitro Conservation Technique for Russian Citrus limon . Agric Res 3, 279–283 (2014). https://doi.org/10.1007/s40003-014-0127-5

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  • DOI: https://doi.org/10.1007/s40003-014-0127-5

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