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Expression analysis of transcripts responsive to osmotic stress in Deschampsia antarctica Desv.

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Abstract

Deschampsia antarctica Desv., the only monocot species thriving in the tough conditions of the Antarctic region, is surrounded by regions with snow and ice where available amount of liquid water is very limited. Considering the species as an invaluable resource for the identification of genes associated with tolerance to osmotic stress, we have characterized the physiological response and the changes in gene expression induced by PEG treatment using a cDNA macroarray. Since relative chlorophyll contents of leaves decreased to 69 %, proline levels were changed significantly, and expression levels of dehydrin and late embryogenesis abundant protein were significantly up-regulated at 1 day after PEG treatment, we used corresponding plant samples as the material for gene expression analysis. As a result of hybridization and analysis of cDNA macroarray, we identified 44 and 20 transcripts that were up-regulated and down-regulated by PEG treatment respectively, and they were divided into several functional categories. Most of the up-regulated transcripts are involved in ‘Response to stimulus’, ‘Amino acid metabolism’, and ‘Respiration’. On the other hand, most of the down-regulated transcripts are involved in ‘Photosynthesis’ and ‘Translation’. Among the up-regulated transcripts, ∆-1-pyrroline-5-carboxylate synthase 2 gene has shown the highest expression level at 1 day after PEG treatment suggesting its possible role as a main player for proline biosynthesis in D. antarctica under osmotic stress. Collectively, these data suggest that the transcripts differentially expressed under osmotic stress may represent candidate genes for dissecting molecular mechanism of adaptation to extreme environment for plants.

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References

  • Alberdi M, Bravo LA, Guitiérrez AH, Gidekel M, Corcuera LJ (2002) Ecophysiology of Antarctic vascular plants. Physiol Plant 115:479–486

    Article  CAS  PubMed  Google Scholar 

  • Andjelkovic V, Thompson R (2006) Changes in gene expression in maize kernel in response to water and salt stress. Plant Cell Rep 25:71–79

    Article  CAS  PubMed  Google Scholar 

  • Cellier F, Conéjéro G, Casse F (2000) Dehydrin transcript fluctuations during a day/night cycle in drought-stressed sunflower. J Exp Bot 51:299–304

    Article  CAS  PubMed  Google Scholar 

  • Chaves MM, Maroco JP, Pereira JS (2003) Understanding plant responses to drought—from genes to the whole plant. Funct Plant Biol 30:239–264

    Article  CAS  Google Scholar 

  • Chaves MM, Flexas J, Pinheiro C (2007) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot 103:551–560

    Article  Google Scholar 

  • Close TJ (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plant 97:795–803

    Article  CAS  Google Scholar 

  • Dugas DV, Monaco MK, Olsen A, Klein RR, Kumari S, Ware D, Klein PE (2011) Functional annotation of the transcriptome of Sorghum bicolor in response to osmotic stress and abscisic acid. BMC Genom 12:514

    Article  CAS  Google Scholar 

  • Edwards JA, Lewis-Smith RI (1988) Photosynthesis and respiration of Colobanthus quitensis and Deschampsia antarctica from the maritime Antarctic. BAS Bull 81:43–63

    Google Scholar 

  • Fu BY, Xiong JH, Zhu LH, Zhao XQ, Xu HX, Gao YM, Li YS, Xu JL, Li ZK (2007) Identification of functional candidate genes for drought tolerance in rice. Mol Genet Genomics 278:599–609

    Article  CAS  PubMed  Google Scholar 

  • Giraud E, Ho LH, Clifton R, Carroll A, Estavillo G, Tan YF, Howell KA, Ivanova A, Pogson BJ, Millar AH, Whelan J (2008) The absence of ALTERNATIVE OXIDASE1a in Arabidopsis results in acute sensitivity to combined light and drought stress. Plant Physiol 147:595–610

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Habib H, Fazili KM (2007) Plant protease inhibitors: a defense strategy in plants. Biotechnol Mol Biol Rev 2:68–85

    Google Scholar 

  • Hoekstra FA, Golovina EA, Buitink J (2001) Mechanisms of plant desiccation tolerance. Trends Plant Sci 6:431–438

    Article  CAS  PubMed  Google Scholar 

  • Hur J, Jung KH, Lee CH, An GH (2004) Stress-inducible OsP5CS2 gene is essential for salt and cold tolerance in rice. Plant Sci 167:417–426

    Article  CAS  Google Scholar 

  • Kasukabe Y, He L, Nada K, Misawa S, Ihara I, Tachibana S (2004) Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana. Plant Cell Physiol 45:712–722

    Article  CAS  PubMed  Google Scholar 

  • Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawai K, Galbraith D, Bohnert HJ (2001) Gene expression profiles during the initial phase of salt stress in rice. Plant Cell 13:889–905

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kishor KPB, Hong Z, Miao GH, Hu CAA, Verma DPS (1995) Overexpression of Δ-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol 108:1387–1394

    PubMed Central  CAS  PubMed  Google Scholar 

  • Lee H, Cho HH, Kim IC, Yim JH, Lee HK, Lee YK (2008) Expressed sequence tag analysis of Antarctic hairgrass Deschampsia antarctica from King George Island, Antarctica. Mol Cells 25:258–264

    CAS  PubMed  Google Scholar 

  • Lee H, Kim JH, Park M, Kim I-C, Yim JH, Lee HK (2010) Reference genes validation for qPCR normalization in Deschampsia antarctica during abiotic stresses. Antarct Sci 22:477–484

    Article  Google Scholar 

  • Lee J, Noh EK, Choi H-S, Shin SC, Park H, Lee H (2013) Transcriptome sequencing of the Antarctic vascular plant Deschampsia antarctica Desv. under abiotic stress. Planta 237:823–836

    Article  CAS  PubMed  Google Scholar 

  • Lichtenthaler H, Wellburn A (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592

    Article  CAS  Google Scholar 

  • Michel BE, Kaufmann MR (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiol 51:914–916

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Mochida K, Kawaura K, Shimosaka E, Kawakami N, Shin-I T, Kohara Y, Yamazaki Y, Ogihara Y (2006) Tissue expression map of a large number of expressed sequence tags and its application to in silico screening of stress response genes in common wheat. Mol Genet Genomics 276:304–312

    Article  CAS  PubMed  Google Scholar 

  • Moon BY, Higashi S, Gombos Z, Murata N (1995) Unsaturation of the membrane lipids of chloroplasts stabilizes the photosynthetic machinery against low-temperature photoinhibition in transgenic tobacco plants. Proc Natl Acad Sci USA 92:6219–6223

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394

    Article  CAS  Google Scholar 

  • Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K (2002) Monitoring the expression profiles of 7,000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J 31:279–292

    Article  CAS  PubMed  Google Scholar 

  • Singh A, Sahi C, Grover A (2009) Chymotrypsin protease inhibitor gene family in rice: genomic organization and evidence for the presence of a bidirectional promoter shared between two chymotrypsin protease inhibitor genes. Gene 428:9–19

    Article  CAS  PubMed  Google Scholar 

  • Smirnoff N (1998) Plant resistance to environmental stress. Curr Opin Biotechnol 9:214–219

    Article  CAS  PubMed  Google Scholar 

  • Strizhov N, Abrahám E, Okrész L, Blickling S, Zilberstein A, Schell J, Koncz C, Szabados L (1997) Differential expression of two P5CS genes controlling proline accumulation during salt-stress requires ABA and is regulated by ABA1, ABI1 and AXR2 in Arabidopsis. Plant J 12:557–569

    Article  CAS  PubMed  Google Scholar 

  • Su M, Li XF, Ma XY, Peng XJ, Zhao AG, Cheng LQ, Chen SY, Liu GS (2011) Cloning two P5CS genes from bioenergy sorghum and their expression profiles under abiotic stresses and MeJA treatment. Plant Sci 181:652–659

    Article  CAS  PubMed  Google Scholar 

  • Talamè V, Ozturk NZ, Bohnert HJ, Tuberosa R (2007) Barley transcript profiles under dehydration shock and drought stress treatments: a comparative analysis. J Exp Bot 58:229–240

    Article  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Umbach AL, Fiorani F, Siedow JN (2005) Characterization of transformed Arabidopsis with altered alternative oxidase levels and analysis of effects on reactive oxygen species in tissues. Plant Physiol 139:1806–1820

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Verdoy D, De la Pena TC, Redondo FJ, Lucas MM, Pueyo JJ (2006) Transgenic Medicago truncatula plants that accumulate proline display nitrogen-fixing activity with enhanced tolerance to osmotic stress. Plant Cell Environ 29:1913–1923

    Article  CAS  PubMed  Google Scholar 

  • Verslues PE, Sharma S (2010) Proline metabolism and its implications for plant-environment interaction. Arabidopsis Book 8:e014

    Article  Google Scholar 

  • Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218:1–14

    Article  CAS  PubMed  Google Scholar 

  • Wang D, Pan Y, Zhao X, Zhu L, Fu B, Li Z (2011) Genome-wide temporal–spatial gene expression profiling of drought responsiveness in rice. BMC Genom 12:149

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by Korea Polar Research Institute (Grants PE13020 and PE13040).

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The authors have declared that no conflicts of interest exist.

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Authors and Affiliations

  1. Division of Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon, 406-840, Korea

    Jungeun Lee, Hyoungseok Lee, Eun Kyeung Noh, Mira Park, Hyun Park, Ji Hyun Kim, Il-Chan Kim & Joung Han Yim

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  1. Jungeun Lee
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  2. Hyoungseok Lee
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  3. Eun Kyeung Noh
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  4. Mira Park
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  5. Hyun Park
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Correspondence to Hyoungseok Lee.

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Lee, J., Lee, H., Noh, E.K. et al. Expression analysis of transcripts responsive to osmotic stress in Deschampsia antarctica Desv.. Genes Genom 36, 283–291 (2014). https://doi.org/10.1007/s13258-013-0166-5

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