Abstract
Functional properties of the Lactobacillus fermentum CFR 2195, isolated from healthy infant feces have been evaluated. The adherence of L. fermentum to HT-29 and Caco-2 cell-lines were found to be 197.66 ± 15.62 and 100.33 ± 15.69 per 100 cells, respectively. The effect of different concentrations of FOS (0.5, 1.0, 1.5, 2.0 and 2.5%) on the growth rate of L. fermentum was checked and 2.0% FOS was selected for further studies. The synbiotic preparation containing L. fermentum and FOS exhibited significant antimicrobial activity against a few tested common food borne pathogens. The proteolytic activity of the L. fermentum was significant and the total amino acid content in milk fermented with L. fermentum was 555 mg/l. In addition, it was found to produce 29.45 ng vitamin B12/g dry biomass in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bernet MF, Brassart D, Neeser JR, Servin AL (1993) Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogens-cell interactions. Appl Environ Microbiol 59:4121–4128
Chopin A (1993) Organization and regulation of genes for amino acid biosynthesis in lactic acid bacteria. FEMS Microbiol Rev 12:21–37
Coconnier MH, Klaenhammer T, Kerneis S, Bernet MF, Servin A (1992) Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucus-secreting cell lines in culture. Appl Environ Microbiol 58:2034–2039
Collins MD, Gibson GR (1999) Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut. Am J Clin Nutr 69:1052S–1057S
Fooks LJ, Gibson GR (2002) In vitro investigations of the effect of probiotics and prebiotics on selected human intestinal pathogens. FEMS Microbiol Ecol 39:67–75
Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota—introducing the concept of prebiotics. J Nutr 125:1401–1412
Girishkumar B, Prapulla SG (2010) Beneficial properties of lactic acid bacteria isolated from the breast fed infants fecal flora: in vitro evidences. Asian J Microbiol Biotechnol Environ Sci 12:887–897
Handan K, Robert WH (2000) Fermentation of Fructooligosaccharides by lactic acid bacteria and bifidobacteria. Appl Environ Microbiol 2682–2684
Heudi O, Kilinc T, Fontannaz P, Marleyc E (2006) Determination of vitamin B12 in food products and in premixes by reverse phase high performance liquid chromatography and immunoaffinity extraction. J Chromatogr A 1101:63–68
Joshi VK, Sharma S (2010) Preparation and evaluation of sauces from lactic acid fermented vegetables. J Food Sci Technol 47:214–218
Lee YK, Salminen S (1995) The coming of age of probiotics. Trends Food Sci Technol 6:241–245
Lenaerts K, Bouwman FG, Lamers WH, Renes J, Mariman EC (2007) Comparative proteomic analysis of cell lines and scrapings of the human intestinal epithelium. BMC Genomics 8:1–14
Macfarlane GT, Gibson GR (1997) Carbohydrate fermentation, energy transduction and gas metabolism in the human large intestine. In: Mackie RI, White BA (eds) Gastrointestinal microbiology, vol 1: gastrointestinal ecosystems and fermentations. Chapman and Hall, London, pp 269–318
Madhu AN, Giribhattanavar P, Narayan MS, Prapulla SG (2010) Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC-MS, immunological and microbiological techniques. Biotechnol Lett 32:503–506
Madhukumar MS, Muralikrishna G (2011) Fermentation of xylooligosaccharides obtained from wheat bran and Bengal gram husk by lactic acid bacteria and bifidobacteria. J Food Sci Technol. doi:10.1007/S13197-010-0226-7
Poonawalla FM, Iyengar MRS (1965) Microbiological assay of vitamin B12 in presence of tetracycline. Appl Microbiol 13:755–756
Salminen S, Isolauri E, Salminen E (1996) Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful stains and future challenges. Antonie Leeuwenhoek 70:251–262
Servin AL, Coconnier MH (2003) Adhesion of probiotic strains to the intestinal mucosa and interaction with pathogens. Best Pract Res Clin Gastroenterol 17:741–754
Sridevi J, Halami PM, Vijayendra SVN (2010) Selection of starter cultures for idli batter fermentation and their effect on quality of idlis. J Food Sci Technol 47:557–563
Stabler SP, Allen RH (2004) Vitamin B12 deficiency as a worldwide problem. Annu Rev Nutr 24:299–326
Thomas TD, Mills OE (1981) Proteolytic enzymes of starter bacteria. Neth Milk Dairy J 35:255–273
Tuomola E, Salminen SJ (1998) Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures. Int J Food Microbiol 41:45–51
Wang B, Wei H, Yuan J, Li Q, Li Y, Li N, Li J (2008) Identification of a surface protein from Lactobacillus reuteri JCM1081 that adheres to porcine gastric mucin and human enterocyte-like HT-29 cells. Curr Microbiol 57:33–38
Zhao R, Sun J, Mo H, Zhu Y (2007) Analysis of functional properties of Lactobacillus acidophilus. World J Microbiol Biotechnol 23:195–200
Acknowledgement
The authors are thankful to Director, CFTRI, Mysore, India for supporting the work. Mr. Girishkumar B is grateful to CSIR for the Senior Research Fellowship. Dr. Vijayan, HOD, Department of Biotechnology, JSS College of Pharmacy, Ooty is kindly acknowledged for his help in cell-lines studies.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
ESM 1
(DOCX 11 kb)
Rights and permissions
About this article
Cite this article
Basavanna, G., Prapulla, S.G. Evaluation of functional aspects of Lactobacillus fermentum CFR 2195 isolated from breast fed healthy infants’ fecal matter. J Food Sci Technol 50, 360–366 (2013). https://doi.org/10.1007/s13197-011-0345-9
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13197-011-0345-9