Abstract
Simple sequence repeat (SSR) markers were developed for Cryptomeria japonica from 55,530 expressed sequence tags (ESTs). Of the 219 designed primer pairs, 176 showed clear PCR amplification products and 27 of these revealed polymorphisms in six individuals sampled at sites across Japan. When these 27 loci were examined in 48 individuals from a single population, the number of alleles and the Polymorphism Information Content (PIC) ranged from 2 to 17 and 0.09 to 0.86, respectively. The average PIC value obtained from EST–SSR markers (0.40) was lower than that from genomic SSR markers (0.67 and 0.62). We observed a significant departure from Hardy–Weinberg equilibrium in five of the 27 loci. These EST–SSR markers will be useful for the evaluation of genetic variation in C. japonica and for genetic mapping.
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Acknowledgments
The authors would like to thank Drs. Maki Saito and Hideaki Taira for providing samples. We are also grateful to Mr. Yasuyuki Komatsu for assistance with laboratory work. This work was supported by a Grant-in-Aid (Development of Technologies for Control of Pollen Production by Genetic Engineering) from the Forest Agency of Japan and Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry.
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Moriguchi, Y., Ueno, S., Ujino-Ihara, T. et al. Characterization of EST–SSRs from Cryptomeria japonica . Conservation Genet Resour 1, 373–376 (2009). https://doi.org/10.1007/s12686-009-9086-8
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DOI: https://doi.org/10.1007/s12686-009-9086-8