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Optimization of Inocula Conditions for Enhanced Biosurfactant Production by Bacillus subtilis SPB1, in Submerged Culture, Using Box–Behnken Design

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Abstract

The inoculum age and density can influence considerably the production yield and cost of the fermentation process. Some literature studies report the use of two-stage inocula to enhance metabolite production. In the present study, optimization studies were done in order to define the best inocula conditions supporting a maximum biosurfactant production by Bacillus subtilis SPB1. Hence, by adjusting the levels of the two-stage inocula strategy, lipopeptide production was effectively enhanced to almost 3.4 g/l as estimated gravimetrically. The new defined parameters were as follows; a first inoculum age of 23 h followed by a second inoculum age and size of 4 h and 0.01, respectively. Thereby, we note an improved production as compared to the production yield described under non-optimized inocula conditions reported in our previous work.

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Acknowledgments

This work has been supported by grants from “Tunisian Ministry of Higher Education, Scientific Research and Technology”.

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Authors and Affiliations

  1. Unité «Enzymes et Bioconversion», National School of Engineers of Sfax, ENIS, BP W 3038, Sfax, Tunisia

    Ines Mnif, Semia Ellouze-Chaabouni & Dhouha Ghribi

  2. Higher Institute of Biotechnology of Sfax, Sfax, Tunisia

    Ines Mnif & Dhouha Ghribi

Authors
  1. Ines Mnif
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  2. Semia Ellouze-Chaabouni
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  3. Dhouha Ghribi
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Correspondence to Ines Mnif.

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Mnif, I., Ellouze-Chaabouni, S. & Ghribi, D. Optimization of Inocula Conditions for Enhanced Biosurfactant Production by Bacillus subtilis SPB1, in Submerged Culture, Using Box–Behnken Design. Probiotics & Antimicro. Prot. 5, 92–98 (2013). https://doi.org/10.1007/s12602-012-9113-z

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