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High throughput screening methods for ω-transaminases

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Abstract

Recently, ω-transaminases have been increasingly used to synthesize amine compounds by reductive amination of prochiral ketones which are of high pharmacological significance. However, the conventional methods for evaluating these enzymes are time consuming and have often been regarded as a bottle neck in developing these enzymes as industrial biocatalysts. In the past few years, several high throughput screening methods have been developed for fast evaluation and identification of ω-transaminase. This review summarizes the various methodologies developed for rapidly screening ω-transaminases.

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Authors and Affiliations

  1. School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Korea

    Sam Mathew, Giyoung Shin, Minsu Shon & Hyungdon Yun

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  1. Sam Mathew
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  2. Giyoung Shin
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  4. Hyungdon Yun
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Correspondence to Hyungdon Yun.

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Mathew, S., Shin, G., Shon, M. et al. High throughput screening methods for ω-transaminases. Biotechnol Bioproc E 18, 1–7 (2013). https://doi.org/10.1007/s12257-012-0544-x

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  • DOI: https://doi.org/10.1007/s12257-012-0544-x

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