Abstract
In this study, we examined the seeds of 7 Dactylorhiza taxa (D. nieschalkiorum and D. osmanica var. osmanica) of which 2 are endemic to Turkey (D. euxina var. euxina, D. iberica, D. romana subsp. romana, D. saccifera, D. urvilleana) in terms of morphological, micromorphological and morphometric properties. We analyzed the seeds using standard procedures of light microscopy and electron microscopy (SEM). The seeds collected from different localities were examined in terms of 22 characters, such as seed shape and size, length/width ratio, embryo volume, seed/embryo volume, percent air space, position of the embryo and seed surface features were analyzed. Furthermore, the morphometric measurements showed that some diagnostic characters could be important for these taxa. As a result of the study, the dendograms were formed based on the numerical analysis and important differences were determined in terms of examined characters among taxa. The characters of the seeds of genus Dactylorhiza are characteristic even in species level. The seed properties identified would help researchers on genus-related problems.
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References
Arditti J (1967) Factors affecting the germination of orchid seeds. Bot Rev 33:1–97
Arditti J (1979) Aspects of the physiology of orchids. Adv Bot Res 7:241–665
Arditti J (1992) Fundamentals of orchid biology. Johln Wiley & Sons Inc, New York, USA
Arditti J, Ernst R (1984) Physiology of germinating orchid seeds. In: Arditti J (ed) Orchid biology reviews and perspectives, vol III. Cornell University Press, Ithaca, NY, USA, pp 179–222
Arditti J, Ghani AKA (2000) Numerical and physical properties of orchid seeds and their biological implications. New Phytol 145:367–421
Arditti J, Michaud JD, Healey P (1979) Morphometry of orchid seeds. I. Paphiopedilum and native California and related species of Cypripedium. Am J Bot 66:1128–1137
Barthlott W (1976) Morphologie der Samen von Orchideen im Hinblick auf taxonomische und funktionelle Aspekte. In: Senghas K (ed) Proceedings of the 8th World Orchid Conference, Frankfurt, Hamburg, Parey, pp 444–455
Barthlott W, Ziegler B (1981) Mikromorphologie der Samenischalen als svstematisch Merkmal bei Orchideen. Ber Deutsch Bot Ges 94:267–273
Bateman RM (2001) Evolution and classification of European orchids: insights from molecular and morphological characters. J Eur Orch 33:33–119
Bournerias M, Aymonin GG, Bournerias J, Demange M, Demares M, Engel R, Gathoye JL, Gerbaud O, Guillaumin JJ, Jacquet P, Lemoine G, Melki F, Prat D, Quentin P, Tyteca D (1998) Les orchidées de France. Belgique et Luxembourg, Parthenope Collection
Burgeff H (1936) Samenkeimung der Orchideen. Gustav Fischer, Jena, Germany
Chase MW, Pippen JS (1988) Seed morphology in the Oncidiinae and related subtribes (Orchidaceae). Syst Bot 13:313–323
Chemisquy MA, Prevosti FJ, Morrone O (2009) Seed morphology in the tribe Chloraeeae (Orchidaceae): combining traditional and geometric morphometrics. Bot J Linn Soc 160(2):171–183
Clifford HT, Smith WK (1969) Seed morphology and classification of Orchidaceae. Phytomorphology 19:133–139
Darwin C (1904) The various contrivances by which orchids are fertilised by insects. John Murray, London, UK
Delforge P (2001) Guide des orchidees d’Europe, d’Afrique du Nord et du Proche-Orient, 2nd edn. Delachaux et Niestle, Lausanne
Devos N, Raspe O, Jacquemart AL, Tyteca D (2006) On the monophyly of Dactylorhiza Necker ex Nevski (Orchidaceae): is Coeloglossum viride (L.) Hartman a Dactylorhiza. Bot J Linn Soc 152(3):261–269
Gamarra R, Dorda E, Scrugli A, Galan P, Ortunez E (2007) Seed micromorphology in the genus Neotinea Rchb.f. (Orchidaceae, Orchidinae). Bot J Linn Soc 153:133–140
Gamarra R, Galan P, Herrera I, Ortunez E (2008) Seed micromor-phology supports the splitting of Limnorchis from Platanthera (Orchidaceae). Nordic J Bot 26:61–65
Gamarra R, Ortunez E, Sanz E, Esparza I, Galan P (2010) Seeds in subtribe Orchidinae (Orchidaceae): the best morphological tool to support molecular analyses. In: Nimis PL, Vignes Lebbe R (eds) Tools for identifying biodiversity: progress and problems. Edizioni Universitadi Trieste, Trieste, pp 323–326
Gamarra R, Ortunez E, Galan PC, Guadano V (2012) Anacamptis versus Orchis (Orchidaceae): seed micromorphology and its taxonomic significance. Plant Syst Evol 298:597–607
Güner A, Aslan S, Ekim T, Vural M, Babaç MT (2012) Türkiye bitkileri listesi (damarlı bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul
Hedren M (2001) Conservation priorities in Dactylorhiza, a taxonomically complex genus. Lindleyana 16:17–25
Inda LA, Pimentel M, Chase MW (2010) Chalcone synthase variation and phylogeneticrelationships in Dactylorhiza (Orchidaceae). Bot J Linn Soc 163:155–165
Krıshna-Swamy K, Krıshna HNK, Ram-Akrıshna TM, Ramaswamy SN (2004) Studies on seed morphometry of epiphytic orchids from Western Ghats of Karnataka. Taiwania 49:124–140
Kurzweil H (1993) Seed morphology in Southern African Orchidoideae (Orchidaceae). Plant Syst Evol 185:229–247
Kuzminsky E, De Angelis P, Jaoudé RA, Abbruzzese G, Terzoli S, Angelaccio C, De Dato G, Monteverdi MC, Valentini R (2014) Biodiversity of Italian Tamarix spp. populations: their potential as environmental and productive resources. Rend Fis Acc Lincei 25(4):439–452
Molvray M, Kores PJ (1995) Character analysis of the seed coat in Spiranthoideae and Orchidoideae, with special reference to the Diurideae (Orchidaceae). Am J Bot 82:1443–1454
Mrkvicka AC (1994) Anatomie und morphologie der Samen heimischer Orchideenarten. J Eur Orch 26:168–314
Nelson E (1976) Monographie und Ikonographie der Orchidaceen-gattung, III. Dactylorhiza, Zürich
Nordström S, Hedrén M (2009) Genetic diversity and differentiation of allopolyploid Dactylorhiza (Orchidaceae) with particular focus on the D. majalis ssp. Traunsteineri lapponica complex. Biol J Linn Soc 97:52–67
Pedersen HA (1998) Species concept and guidelines for infraspecific taxonomic ranking in Dactylorhiza (Orchidaceae). Nordic J Bot 18:289–310
Pillon Y, Fay MF, Shipunov AB, Chase MW (2006) Species diversity versus phylogenetic diversity: a practical study in the taxonomically difficult genus Dactylorhiza (Orchidaceae). Biol Conserv 129(1):4–13
Plaza L, Fernández I, Juan R, Pastor J, Pujadas A (2004) Micromorphological studies on seeds of Orobanche species from the Iberian Peninsula and the Balearic Islands, and their systematic significance. Ann Bot 94(1):167–178
Rasmussen HN (1995) Terrestrial orchids, from seed to mycotrophic plant. Cambridge University Press, Cambridge, UK
Renz J, Taubenheim G (1984) Dactylorhiza Necker ex Nevski. In: Davis PH (ed) Flora of Turkey and the east Aegean islands, vol 8. Edinburgh University Press, Edinburgh, pp 425–450
Shipunov AB, Bateman RM (2005) Geometric morphometrics as a tool for understanding Dactylorhiza (Orchidaceae) diversity in European Russia. Biol J Linn Soc 85:1–12
Sundermann H (1984) Taxonomie et nomenclature des Orchidees explique a 1’exemple du genre Ophrys. 8eme colloque de la S. F. O. Paris, pp 57–72
Swamy KK, Kumar HNK, Ramakrishna TM, Ramaswamy SN (2004) Studies on seed morphometry of epiphytic orchids from Western Ghats of Karnataka. Taiwania 49:124–140
Tsutsumi C, Yukawa T, Sook Lee N, Shook Lee C, Kato M (2007) Phylogeny and comparative seed morphology or epiphytic and terrestrial species of Liparis (Orchidaceae) in Japan. J Plant Res 120:405–412
Verma J, Sharma K, Thakur K, Sembı JK, Vıj SP (2014) Study on seed morphometry of some threatened Western Himalayan orchids. Turk J Bot 38:234–251
Vij SP (2006) Orchids: ingenuity at its best. In: Proceedings of the 93rd Indian Science Congress, Hyderabad. The Indian Science Congress association, Kolkata, pp 1–26
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This research was funded by a Grant from the Scientific and Technological Research Council of Turkey (TUBITAK, 114Z702).
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Akbulut, M.K., Şenel, G. The seeds micromorphology and morphometry of certain Dactylorhiza (Orchidaceae) species distributed in Turkey. Rend. Fis. Acc. Lincei 27, 679–686 (2016). https://doi.org/10.1007/s12210-016-0549-z
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DOI: https://doi.org/10.1007/s12210-016-0549-z