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Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats

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Abstract

We evaluated associations between the concentrations of heat shock proteins (hsp60 and hsp70) and their respective antibodies, alterations in maternal reproductive performance, and fetal malformations in pregnant rats with hyperglycemia. Mild diabetes (MD) or severe diabetes (SD) was induced in Sprague-Dawley rats prior to mating; non-treated non-diabetic rats (ND) served as controls. On day 21 of pregnancy, maternal blood was analyzed for hsp60 and hsp70 and their antibodies; and fetuses were weighed and analyzed for congenital malformations. Hsp and anti-hsp levels were correlated with blood glucose levels during gestation. There was a positive correlation between hsp60 and hsp70 levels and the total number of malformations (R = 0.5908, P = 0.0024; R = 0.4877, P = 0.0134, respectively) and the number of malformations per fetus (R = 0.6103, P = 0.0015; R = 0.4875, P = 0.0134, respectively). The anti-hsp60 IgG concentration was correlated with the number of malformations per fetus (R = 0.3887, P = 0.0451) and the anti-hsp70 IgG level correlated with the total number of malformations (R = 0.3999, P = 0.0387). Moreover, both hsp and anti-hsp antibodies showed negative correlations with fetal weight. The results suggest that there is a relationship between hsp60 and hsp70 levels and their respective antibodies and alterations in maternal reproductive performance and impaired fetal development and growth in pregnancies associated with diabetes.

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Acknowledgments

We are grateful to Dr. Maria Terezinha Serrão Peraçoli for assistance and helpful discussions. We would also like to thank to Talísia Moreto, technician in the Laboratory of Experimental Research on Gynecology and Obstetrics. The financial support of CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) is gratefully acknowledged.

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Authors and Affiliations

  1. Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil

    Felipe Hiroshi Saito, Débora Cristina Damasceno, Bruna Dallaqua, Marilza Vieira Cunha Rudge, Iracema De Mattos Paranhos Calderon & Steven S. Witkin

  2. Department of Gynecology, University of Sao Paulo Medical School and Hospital das Clínicas, Sao Paulo, Brazil

    Iara Moreno Linhares

  3. Division of Immunology and Infectious Diseases, Department of Obstetrics and Gynecology, Weill Cornell Medical College, New York, NY, USA

    Steven S. Witkin

  4. Department of Obstetrics and Gynecology, Weill Cornell Medical College, 525 East 68th Street, Box 35, New York, NY, 10065, USA

    Steven S. Witkin

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  1. Felipe Hiroshi Saito
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  2. Débora Cristina Damasceno
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  3. Bruna Dallaqua
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Correspondence to Steven S. Witkin.

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Saito, F.H., Damasceno, D.C., Dallaqua, B. et al. Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats. Cell Stress and Chaperones 18, 25–33 (2013). https://doi.org/10.1007/s12192-012-0353-3

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