Skip to main content

Advertisement

SpringerLink
Log in

Advances in RNA Interference Technology and Its Impact on Nutritional Improvement, Disease and Insect Control in Plants

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

This review highlights the advances in the knowledge of RNA interference (RNAi) and discusses recent progress on the functionality of different components RNAi machinery operating in the organisms. The silencing of genes by RNA interference has become the technology of choice for investigation of gene functions in different organisms. The refinement in the knowledge of the endogenous RNAi pathways in plants along with the development of new strategies and applications for the improvement of nutritional value of important agricultural crops through suppression of genes in different plants have opened new vistas for nutritional security. The improvement in the nutritional status of the plants and reduction in the level of toxins or antinutrients was desired for long, but the available technology was not completely successful in achieving the tissue specific regulation of some genes. In the recent years, a number of economically important crop plants have been tested successfully for improving plant nutritional value through metabolic engineering using RNAi. The implications of this technology for crop improvement programs, including nutritional enrichment, reduction of antinutrients, disease, and insect control have been successfully tested in variety of crops with commercial considerations. The enhancement of the nutraceutical traits for the desired health benefits in common crop plants through manipulation of gene expression has been elaborated in this article. The tremendous potential with RNAi technology is expected to revolutionize the modern agriculture for meeting the growing challenges is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Akashi, H., Miyagishi, M., & Taira, K. (2004). Methods in Molecular Biology, 252, 533–543.

    CAS  Google Scholar 

  2. Allen, R., & Lonsdale, D. (1993). The Plant Journal, 3, 261–271.

    Article  CAS  Google Scholar 

  3. Amdam, G. V., Simões, Z. L. P., Guidugli, K. R., Norberg, K., & Omholt, S. W. (2000). BMC Biotechnology, 3, 1–8.

    Article  Google Scholar 

  4. Aronstein, K., Pankiw, T., & Saldivar, E. (2006). Journal of Apiculture Research, 45, 20–24.

    CAS  Google Scholar 

  5. Ashihara, H., & Crozier, A. (2001). Trends in Plant Science, 6, 407–413.

    Article  CAS  Google Scholar 

  6. Auer, C., & Frederick, R. (2009). Trends in Biotechnology, 27(11), 644–651.

    Article  CAS  Google Scholar 

  7. Badisco, L., Marchal, E., Wielendaele, P. V., Verlinden, H., Vleugels, R., Vanden, J., & Broeck, J. V. (2011). Peptides, 32, 573–580.

    Article  CAS  Google Scholar 

  8. Barakate, A., Martin, W., Quigley, F., & Mache, R. (1993). Journal of Molecular Biology, 229, 797–801.

    Article  CAS  Google Scholar 

  9. Bartel, D. P. (2004). Cell, 116, 281–297.

    Article  CAS  Google Scholar 

  10. Baum, J. A. (2007). Nature Biotechnology, 25, 1322–1326.

    Article  CAS  Google Scholar 

  11. Bendahmane, M., & Gronenborn, B. (1997). Plant Molecular Biology, 33, 351–357.

    Article  CAS  Google Scholar 

  12. Byzova, M., Verduyn, C., De Brouwer, D., & De Block, M. (2004). Planta, 218, 379–387.

    Article  CAS  Google Scholar 

  13. Carmell, M. A., Xuan, Z., Zhang, M. Q., & Hannon, G. J. (2002). Genes & Development, 16, 2733–2742.

    Article  CAS  Google Scholar 

  14. Chai, X. J., Wang, P. W., Guan, S. Y., & Xu, Y. W. (2005). Journal of Plant Physiology and Molecular Biology, 31, 625–630.

    CAS  Google Scholar 

  15. Chapman, E. J., & Carrington, J. C. (2007). Nature Reviews Genetics, 8, 884–896.

    Article  CAS  Google Scholar 

  16. Chen, J., Zhang, D., Yao, Q., Zhang, J., Dong, X., Tian, H., Chen, J., & Zhang, W. (2010). Insect Molecular Biology, 19(6), 777–786.

    Article  CAS  Google Scholar 

  17. Chiu, Y. L., & Rana, T. M. (2002). Molecular Cell Biology, 10, 549–561.

    CAS  Google Scholar 

  18. Cigan, A. M., Unger-Wallace, E., & Haug-Collet, K. (2005). The Plant Journal, 43, 929–940.

    Article  CAS  Google Scholar 

  19. Covey, S. N., AI-Kaff, N. S., Langara, A., & Turner, D. S. (1997). Nature, 385, 781–782.

    Article  CAS  Google Scholar 

  20. Crowe, T. C., Seligman, S. A., & Copeland, L. (2000). Journal of Nutrition, 130, 2006–2008.

    CAS  Google Scholar 

  21. Davuluri, G. R., Tuinen, A., & Fraser, P. D. (2005). Nature Biotechnology, 23(7), 890–895.

    Article  CAS  Google Scholar 

  22. Dhankher, O. M., Rosen, B. P., McKinney, E. C., & Meagher, R. B. (2006). Proceedings of the National Academy of Sciences USA, 103, 5413–5418.

    Article  CAS  Google Scholar 

  23. Doench, J. G., & Sharp, P. A. (2004). Genes & Development, 18, 504–511.

    Article  CAS  Google Scholar 

  24. Doench, J. G., Petersen, C. P., & Sharp, P. A. (2003). Genes & Development, 17, 438–442.

    Article  CAS  Google Scholar 

  25. Eady, C. C., Kamoi, T., Kato, M., Noel, G. P., Davis, S., Shaw, M., Kamoi, A., & Imai, S. (2008). Plant Physiology, 147, 2096–2106.

    Article  CAS  Google Scholar 

  26. Elbashir, M. S., Martinez, J., Patkaniowska, A., Lendeckel, W., & Tuschl, T. (2001). European Molecular Biology Organization Journal, 20, 6877–6888.

    CAS  Google Scholar 

  27. Elbashir, S. M., Lendeckel, W., & Tuschl, T. (2001). Genes & Development, 15, 188–200.

    Article  CAS  Google Scholar 

  28. Escobar, M. A., Edwin, L. C., Summerfelt, K. R., & Dandekar, A. M. (2001). Proceedings of the National Academy of Sciences USA, 98, 13437–13442.

    Article  CAS  Google Scholar 

  29. Farazi, T. A., Juranek, S. A., & Tuschl, T. (2008). Development, 135, 1201–1214.

    Article  CAS  Google Scholar 

  30. Filipowicz, W. (2005). Cell, 122, 17–20.

    Article  CAS  Google Scholar 

  31. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998). Nature, 91, 806–811.

    Article  Google Scholar 

  32. Forkmann, G., & Martens, S. (2001). Current Opinion in Biotechnology, 12, 155–160.

    Article  CAS  Google Scholar 

  33. Goldbach, R., Bucher, E., & Prins, M. (2003). Virus Research, 92, 207–212.

    Article  CAS  Google Scholar 

  34. Griebler, M. (2008). Journal of Insect Physiology, 54, 997–1007.

    Article  CAS  Google Scholar 

  35. Guo, S., & Kemphues, K. J. (1995). Cell, 81, 611–620.

    Article  CAS  Google Scholar 

  36. Hammond, S., Bernstein, M. E., Beach, B. D., & Hannon, G. J. (2000). Nature, 404, 293–296.

    Article  CAS  Google Scholar 

  37. Hirai, S., Oka, S., Adachi, E., & Kodama, H. (2007). Plant Cell Reports, 26, 651–659.

    Article  CAS  Google Scholar 

  38. Horiguchi, G. (2004). Differentiation, 72, 65–73.

    Article  CAS  Google Scholar 

  39. Huang, G., Allen, R., Davis, E. L., Baum, T. J., & Hussey, R. S. (2006). Proceedings of the National Academy of Sciences USA, 103, 14302–14306.

    Article  CAS  Google Scholar 

  40. Hutvágner, G., & Zamore, P. D. (2002). Science, 297, 2056–2060.

    Article  CAS  Google Scholar 

  41. Huvenne, H., & Smagghe, G. (2010). A review. Journal of Insect Physiology, 56, 227–235.

    Article  CAS  Google Scholar 

  42. Jinek, M., & Doudna, J. A. (2009). Nature, 457, 405–412.

    Article  CAS  Google Scholar 

  43. Kapoor, S., Kobayashi, A., & Takatsuji, S. (2002). The Plant Cell, 14, 2353–2367.

    Article  CAS  Google Scholar 

  44. Khvorova, A., Reynolds, A., & Jayasena, S. D. (2003). Cell, 115, 209–216.

    Article  CAS  Google Scholar 

  45. Koltunow, A. M., Truettner, J., Cox, K. H., Wallroth, M., & Goldberg, R. B. (1990). The Plant Cell, 2, 1201–1224.

    CAS  Google Scholar 

  46. Kumar, M., Gupta, G. P., & Rajam, M. V. (2009). Journal of Insect Physiology, 55, 273–278.

    Article  CAS  Google Scholar 

  47. Kusaba, M., Miyahara, K., Iida, S., & Fukuoka, H. (2003). The Plant Cell, 15, 1455–1467.

    Article  CAS  Google Scholar 

  48. Lee, Y., Ahn, C., Han, J., Choi, H., Kim, J., Yim, J., Lee, J., Provost, P., Rådmark, O., Kim, S., & Kim, V. N. (2003). Nature, 425, 415–419.

    Article  CAS  Google Scholar 

  49. Lee, Y., Kim, M., Han, J., Yeom, K. H., Lee, S., Baek, S. H., & Kim, V. N. (2004). European Molecular Biology Organization Journal, 23, 4051–4060.

    CAS  Google Scholar 

  50. Lewis, B.P., Burge, C.B., & Bartel, D.P. (2005). Cell, 120, 15–20.

    Article  CAS  Google Scholar 

  51. Lindbo, J. A., Silva-Rosales, L., Proebsting, W. M., & Dougherty, W. G. (1993). The Plant Cell, 5, 1749–1759.

    CAS  Google Scholar 

  52. Lingel, A., Simon, B., & Izaurralde, E. M. (2003). Nature, 426, 465–469.

    Article  CAS  Google Scholar 

  53. Lingel, A., Simon, B., Izaurralde, E., & Sattler, M. (2004). Nature Structural & Molecular Biology, 11, 576–577.

    Article  CAS  Google Scholar 

  54. Liu, Q., Singh, S. P., & Green, A. G. (2002). Plant Physiology, 129, 1732–1743.

    Article  CAS  Google Scholar 

  55. Ma, J. B., Ye, K., & Patel, D. J. (2004). Nature, 429, 318–322.

    Article  CAS  Google Scholar 

  56. Ma, L., Reinhardt, F., Pan, E., Soutschek, J., Bhat, B., Marcusson, E. G., Teruya-Feldstein, J., Bell, G. W., & Weinberg, R. A. (2010). Nature Biotechnology, 28, 341–347.

    Article  CAS  Google Scholar 

  57. Macrae, I. J., & Doudna, J. A. (2007). Current Opinion in Structural Biology, 17, 138–145.

    Article  CAS  Google Scholar 

  58. Macrae, I. J., Zhou, K., Li, F., Repic, A., Brooks, A. N., Cande, W. Z., Adams, P. D., & Doudna, J. A. (2006). Science, 311, 195–198.

    Article  CAS  Google Scholar 

  59. Mao, Y. B., Cai, W. J., Wang, J. W., Hong, G. J., Tao, X. Y., Wang, L. J., Huang, Y. P., & Chen, X. Y. (2007). Nature Biotechnology, 25, 1307–1313.

    Article  CAS  Google Scholar 

  60. Mariani, C., De Beuckeleer, M., Truettner, J., Leemans, J., & Goldberg, R. B. (1990). Nature, 347, 737–741.

    Article  CAS  Google Scholar 

  61. Martinez, J., & Tuschl, T. (2004). Genes & Development, 18, 975–980.

    Article  CAS  Google Scholar 

  62. Martinez, J., Patkaniowska, A., Urlaub, H., Luhrmann, R., & Tuschl, T. (2002). Cell, 110, 563–574.

    Article  CAS  Google Scholar 

  63. Maskarinec, G. (2006). Obesity (Silver Spring), 14, 717–726.

    Article  Google Scholar 

  64. Matranga, C., Tomari, Y., Shin, C., Bartel, D. P., Phillip, D., & Zamor, P. D. (2005). Cell, 123, 607–620.

    Article  CAS  Google Scholar 

  65. Meister, G., & Tuschl, T. (2004). Nature, 431, 343–349.

    Article  CAS  Google Scholar 

  66. Miki, D., & Shimamoto, K. (2004). Plant & Cell Physiology, 45, 490–495.

    Article  CAS  Google Scholar 

  67. Miyajima, I., Maehara, T., & Kage, T. (1991). Japanese Society for Horticultural Science, 60, 409–414.

    Article  CAS  Google Scholar 

  68. Miyoshi, K., Tsukumo, H., Nagami, T., Siomi, H., & Siomi, M. C. (2005). Genes & Development, 19, 2837–2848.

    Article  CAS  Google Scholar 

  69. Montgomery, M. K., & Fire, A. (1998). Trends in Genetics, 14(7), 255–258.

    Article  CAS  Google Scholar 

  70. Muir, S. R., Collins, G. J., Robinson, S., Hughes, S., & Bovy, A. (2001). Nature Biotechnology, 19, 470–474.

    Article  CAS  Google Scholar 

  71. Napoli, C., Lemieux, C., & Jorgensen, R. (1990). The Plant Cell, 2, 279–289.

    CAS  Google Scholar 

  72. Niggeweg, R., Michael, A. J., & Martin, C. (2004). Nature Biotechnology, 22, 746–754.

    Article  CAS  Google Scholar 

  73. Nishihara, M., Nakatsuka, T., & Yamamuras, S. (2005). FEBS Letters, 579, 6074–6078.

    Article  CAS  Google Scholar 

  74. Noris, E., Lucioli, A., Tavazza, R., Caciagli, P., Accotto, G. P., & Tavazza, M. (2004). Journal of General Virology, 85, 1745–1749.

    Article  CAS  Google Scholar 

  75. Nowotny, M., Gaidamakov, S. A., Crouch, R. J., & Yang, W. (2005). Cell, 121, 1005–1016.

    Article  CAS  Google Scholar 

  76. Nunes, F. M. F., & Simões, Z. L. P. (2009). Insect Biochemistry and Molecular Biology, 39, 157–160.

    Article  CAS  Google Scholar 

  77. Nykänen, A., Haley, B., & Zamore, P. D. (2001). Cell, 107, 309–321.

    Article  Google Scholar 

  78. Obbard, D. J., Gordon, K. H. J., Buck, A. H., & Jiggins, F. M. (2009). Philosophical Transactions of the Royal Society, 364, 99–115.

    Article  CAS  Google Scholar 

  79. Ogita, S., Uefuji, H., Morimoto, M., & Hogita, S. (2004). Plant Molecular Biology, 54, 931–941.

    Article  CAS  Google Scholar 

  80. Panstruga, R. (2003). Molecular Biology Reports, 30, 135–140.

    Article  CAS  Google Scholar 

  81. Parker, J. S., Roe, S. M., & Barford, D. (2004). European Molecular Biology Organization Journal, 23, 4727–4237.

    CAS  Google Scholar 

  82. Parker, J. S., Roe, S. M., & Barford, D. (2005). Nature, 434, 663–666.

    Article  CAS  Google Scholar 

  83. Pham, J. W., Pellino, J. L., Lee, Y. S., Carthew, R. W., & Sontheimer, E. J. (2004). Cell, 117, 83–94.

    Article  CAS  Google Scholar 

  84. Pooggin, M., Shivaprasad, P. V., Veluthambi, K., & Hohn, T. (2003). Nature Biotechnology, 21, 131–132.

    Article  CAS  Google Scholar 

  85. Price, D. R. G., & Gatehouse, J. A. (2008). Trends in Biotechnology, 26, 393–400.

    Article  CAS  Google Scholar 

  86. Rajagopal, R., Sivakumar, S., Agrawal, N., Malhotra, P., & Bhatnagar, R. K. (2002). Journal of Biological Chemistry, 277, 46849–46851.

    Article  CAS  Google Scholar 

  87. Ratcliff, F., Harrison, B. D., & Baulcombe, D. C. (1997). Science, 276, 1558–1560.

    Article  CAS  Google Scholar 

  88. Regina, A., Kosar-Hashemi, B., Li, Z., Rampling, L., Cmiel, M., & Gianibelli, C. (2006). Proceedings of the National Academy of Sciences USA, 103, 3546–3551.

    Article  CAS  Google Scholar 

  89. Schwarz, D. S., Tomari, Y., & Zamore, P. D. (2004). Current Biology, 14, 787–791.

    Article  CAS  Google Scholar 

  90. Segal, G., Song, R., & Messing, J. (2003). Genetics, 165, 387–397.

    CAS  Google Scholar 

  91. Seggerson, K., Tang, L., & Moss, E. G. (2002). Developmental Biology, 243, 215–225.

    Article  CAS  Google Scholar 

  92. Sharp, P. A. (1999). Genes & Development, 13, 139–141.

    Article  CAS  Google Scholar 

  93. Shu, Y. H., Wang, J. W., Lu, K., Zhou, J. L., Zhou, Q., & Zhang, G. R. (2011). Insect Molecular Biology, 20(1), 61–73.

    Article  CAS  Google Scholar 

  94. Sijen, T., Fleenor, J., Simmer, F., Thijssen, K. L., Parrish, S., Timmons, L., & Plasterk, R. H. A. (2001). Cell, 107, 465–476.

    Article  CAS  Google Scholar 

  95. Smith, N. A., Singh, S. P., Wang, M. B., Stoutjesdijk, P. A., Green, A. G., & Waterhouse, P. M. (2000). Nature, 407, 319–320.

    Article  CAS  Google Scholar 

  96. Song, J. J., Liu, J., Tolia, N. H., Schneiderman, J., Smith, S. K., Hannon, G. J., & Joshua-Tor, L. (2004). Science, 305, 1434–1437.

    Article  CAS  Google Scholar 

  97. Sunilkumar, G., Campbell, L. M., Puckhaber, L., Stipanovic, R. D., & Rathore, K. S. (2006). Proceedings of the National Academy of Sciences USA, 103(48), 18054–18059.

    Article  CAS  Google Scholar 

  98. Tabashnik, B. E., Gassmann, A. J., Crowder, D. W., & Carrière, Y. (2008). Nature Biotechnology, 26(2), 199–2002.

    Article  CAS  Google Scholar 

  99. Takaiwa, F., Takagi, H., Hirose, S., & Wakasa, Y. (1991). Plant Molecular Biology, 17, 875–885.

    Article  CAS  Google Scholar 

  100. Tang, G., Galili, G., & Zhuang, X. (2007). Metabolomics, 3, 357–369.

    Article  CAS  Google Scholar 

  101. Tenllado, F., Gonzalez-Jara, P., Martinez-Garcia, B., & Atencio, F. A. (2003). BMC Biotechnology, 3, 3–14.

    Article  Google Scholar 

  102. Terenius, O. (2011). Journal of Insect Physiology, 57, 231–245.

    Article  CAS  Google Scholar 

  103. Terra, W. R., & Cristofoletti, P. T. (1996). Comparative Biochemistry and Physiology, 113(4), 725–730.

    Article  Google Scholar 

  104. Thao, N. P., Chen, L., Nakashima, A., Hara, S., Umemura, K., Takahashi, A., Shirasu, K., Kawasaki, T., & Shimamoto, K. (2007). The Plant Cell, 19, 4035–4045.

    Article  CAS  Google Scholar 

  105. Timmons, L., Court, D. L., & Fire, A. (2001). Gene, 263(1–2), 103–112.

    Article  CAS  Google Scholar 

  106. Timmons, L., & Fire, A. (1998). Nature, 395, 854–854.

    Article  CAS  Google Scholar 

  107. Turner, C. T., Davy, M. W., MacDiarmid, R. M., Plummer, K. M., Birch, N. P., & Newcomb, R. D. (2006). Insect Molecular Biology, 15, 383–391.

    Article  CAS  Google Scholar 

  108. Ullu, E., Tschudi, C., & Chakraborty, T. (2004). Cell Microbiology, 6, 509–519.

    Article  CAS  Google Scholar 

  109. Van der Krol, A. R., Mur, L. A., Beld, M., Mol, J. N. M., & Stuitje, A. R. (1990). The Plant Cell, 2, 291–299.

    Google Scholar 

  110. Voinnet, O. (2008). Trends in Plant Science, 13, 317–328.

    Article  CAS  Google Scholar 

  111. Voinnet, O., & Baulcombe, D. C. (1997). Nature, 389(6651), 553–553.

    Article  CAS  Google Scholar 

  112. Walshe, D. P., Lehane, S. M., Lehane, M. J., & Haines, L. R. (2009). Insect Molecular Biology, 18, 11–19.

    Article  CAS  Google Scholar 

  113. Wang, M. B., Abbott, D. C., & Waterhouse, P. M. (2000). Molecular Plant Pathology, 1, 347–356.

    Article  CAS  Google Scholar 

  114. Wang, X. B. (2010). Proceedings of the National Academy of Sciences USA, 107(1), 1484–1489.

    Google Scholar 

  115. Wang, Y., Kheir, M. M., Chai, Y., Hu, J., & Xing, D. (2011). PloS One, 6(4), 1–10.

    Google Scholar 

  116. Waterhouse, P. M., Graham, H. W., & Wang, M. B. (1998). Proceedings of the National Academy of Sciences USA, 95, 13959–13964.

    Article  CAS  Google Scholar 

  117. Wesley, S. V., Helliwell, C. A., Smith, N. A., Wang, M. B., Rouse, D. T., Liu, Q., Gooding, P. S., Singh, S. P., Abbott, D., & Stoutjesdijk, P. A. (2001). The Plant Journal, 27, 581–590.

    Article  CAS  Google Scholar 

  118. Williams, C. L. (1995). Journal of the American Dietetic Association, 95, 1140–1146.

    Article  CAS  Google Scholar 

  119. Wilson, Z. A., Morroll, S. M., Dawson, J., Swarup, R., & Tighe, P. J. (2001). The Plant Journal, 28, 27–39.

    Article  CAS  Google Scholar 

  120. Winston, W. M., Molodowitch, C., & Hunter, C. P. (2002). Science, 295, 2456–2459.

    Article  CAS  Google Scholar 

  121. Winston, W. M., Sutherlin, M., Wright, A. J., Feinberg, E. H., & Hunter, C. P. (2007). Proceedings of the National Academy of Sciences USA, 104, 10565–10570.

    Article  CAS  Google Scholar 

  122. Xiong, A. S. H., Yao, Q. H., & Peng, R. H. (2004). Plant Cell Reports, 23, 639–646.

    Article  CAS  Google Scholar 

  123. Xu, W., & Han, Z. (2008). Journal of Insect Science, 8, 30.

    Article  Google Scholar 

  124. Yadav, B. C., Veluthambi, K., & Subramaniam, K. (2006). Molecular and Biochemical Parasitology, 148, 219–222.

    Article  CAS  Google Scholar 

  125. Zhang, H., Fabrice, A., Lukasz, K., Westhof, J. E., & Filipowicz, W. (2004). Cell, 118, 57–68.

    Article  CAS  Google Scholar 

  126. Zhang, H., Kolb, F. A., Brondani, V., Billy, E., & Filipowicz, W. (2002). European Molecular Biology Organization Journal, 21, 5875–5885.

    CAS  Google Scholar 

  127. Zhang, P., Vanderschuren, H., Fütterer, J., & Gruissem, W. (2005). Plant Biotechnology Journal, 3, 385–397.

    Article  CAS  Google Scholar 

  128. Zhao, M. M. (2006). Acta Biochimica et Biophysica Sinica, 38, 22–28.

    Article  CAS  Google Scholar 

  129. Zhao, Y. Y., Liu, F., Yang, G., & You, M. S. (2011). Insect Molecular Biology, 20(1), 97–104.

    Article  CAS  Google Scholar 

  130. Zhao, Y. Y., Yang, G., Wang-Pruski, G., & You, M. S. (2008). European Journal of Entomology, 105, 815–822.

    CAS  Google Scholar 

Download references

Author information

Author notes

  1. Rajan Katoch

    Present address: Department of Entomology, Purdue University, West Lafayette, IN, 47907, USA

Authors and Affiliations

  1. Biochemistry Laboratory, Department of Crop Improvement, College of Agriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India, 176062

    Rajan Katoch

  2. Department of Biosciences, Punjab Agricultural University, Ludhiana, India, 141004

    Neelam Thakur

Authors
  1. Rajan Katoch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Neelam Thakur
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rajan Katoch.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Katoch, R., Thakur, N. Advances in RNA Interference Technology and Its Impact on Nutritional Improvement, Disease and Insect Control in Plants. Appl Biochem Biotechnol 169, 1579–1605 (2013). https://doi.org/10.1007/s12010-012-0046-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-0046-5

Keywords

Search

Navigation