Abstract
Cane molasses (CM), an agro-industrial by-product, was first examined for docosahexaenoic acid (DHA) production by Schizochytrium sp. Cell dry weight as 21.94 g/L at treated CM cultivation was similar to that at pure glucose (26.7 g/L) cultivation. Batch fermentation at different initial CM concentration showed that DHA percentage could reach 47.51% at 10 g/L CM but only 37.90% at 70 g/L CM. By analyzing the fermentation process, monosodium glutamate might be a positive agent for effective DHA production. Finally, monosodium glutamate and malic acid were introduced to the fed-batch fermentation for effective DHA production.
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References
C. Agostoni, E. Riva, S. Trojan, R. Bellu and M. Giovannini, Lancet., 346, 638 (1995).
L. Sijtsma and M. E. Swaaf, Appl. Microbiol. Biotechnol., 64, 146 (2004).
E. Ganuza, A. J. Anderson and C. Ratledge, Biotechnol. Lett., 30, 1559 (2008).
Y. Liang, N. Sarkany, Y. Cui and J.W. Blackburn, Bioresour. Technol., 101, 6745 (2010).
P. Unagul, C. Assantachai, S. Phadungruengluij, M. Suphantharika, M. Tanticharoen and C. Verduyn, Bioresour. Technol., 98, 281 (2007).
Y. Liang, N. Sarkany, Y. Cui, J. Yesuf, J. Trushenski and J.W. Blackburn, Bioresour. Technol., 101, 3023 (2010).
Y. P. Liu, P. Zheng, Z. H. Sun, Y. Ni, J. J. Dong and L. L. Zhu, Bioresour. Technol., 99, 1736 (2010).
H. A. El-Enshasy, N. A. Mohamed, M.A. Farid and A. I. El-Diwany, Bioresour. Technol., 99, 4263 (2008).
N. Li, Z.N. Deng, Y. L. Qin, C. L. Chen and Z.Q. Liang, Food Technol. Biotechnol., 46, 73 (2008).
L. J. Ren, H. Huang, A. H. Xiao, M. Lian and X. J. Ji, Bioproc. Biosyst. Eng., 32, 837 (2009).
Q. Ye, X. M. Li, M. Yan, H. Cao, L. Xu, Y.Y. Zhang, Y. Chen, J. Xiong, P. K. Ouyang and H. J. Ying, Appl. Microbiol. Biotechnol., 87, 517 (2010).
G. L. Miller, Anal. Chem., 31, 426 (1959).
L. J. Ren, X. J. Ji, H. Huang, L. Qu, Y. Feng, Q. Q. Tong and P. K. Ouyang, Appl. Microbiol. Biotechnol., 87, 1649 (2010).
L. J. Yu, W. M. Qin, W. Z. Lan, P. P. Zhou and M. Zhu, Bioresour. Technol., 88, 265 (2003).
C. Ratledge, Biochimie, 86, 807 (2004).
M. Edmund, J. Wise and G. B. Eric, Proc. Natl. Acad Sci., 52(5), 1255 (1964).
R. T. Wedding, Plant Physiol., 90, 367 (1989).
W. Z. Lan, W. M. Qin and L. J. Yu. Lett. Appl. Microbiol., 35, 357 (2002).
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Ren, LJ., Li, J., Hu, YW. et al. Utilization of cane molasses for docosahexaenoic acid production by Schizochytrium sp. CCTCC M209059. Korean J. Chem. Eng. 30, 787–789 (2013). https://doi.org/10.1007/s11814-013-0020-0
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DOI: https://doi.org/10.1007/s11814-013-0020-0