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Temporary Increase of PPAR-γ and Transient Expression of UCP-1 in Stromal Vascular Fraction Isolated Human Adipocyte Derived Stem Cells During Adipogenesis

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Lipids

Abstract

In this study, cells from the stromal vascular fraction of human subcutaneous tissues were induced to differentiate toward adipose cells in vitro for 2 weeks. During adipogenic differentiation, we followed the chronological changes in their morphology with Coherent anti-Stokes Raman scattering (CARS) microscopy and checked the PPAR-γ and UCP-1 expression with RT-PCR. On day 4 after inducing adipogenic differentiation, CARS imaging showed multiple small lipid droplets (LD) distributed peripherally along the cellular membrane. PPAR-γ began to express at this time and increased until day 14 at a steady rate. On day 7, the cells appeared as brown adipocytes with numerous small LD throughout the cytoplasm, and the mRNA level of UCP-1 rose abruptly by 6- to 7-fold. After an additional 7 days, CARS imaging showed the development of a large LD, which is characteristic of white adipocytes, and the mRNA level of UCP-1 slumped significantly. These results demonstrate the possibility that ADSC pass through a brown adipocyte-like stage while differentiating into white adipocytes.

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Abbreviations

ADSC:

Adipose-derived stem cell

BAT:

Brown adipose tissue

CARS:

Coherent anti-Stokes Raman scattering

DMEM:

Dulbecco’s modified Eagle’s medium

FBS:

Fetal bovine serum

IBMX:

Isobutyl-methylxanthine

LD:

Lipid droplet

PBS:

Phosphate-buffered saline

PPAR-γ:

Peroxisome proliferator-activated receptor-γ

SVF:

Stromal vascular fraction

UCP-1:

Mitochondria-uncoupling protein-1

WAT:

White adipose tissue

References

  1. Avram A, Avram M, James W (2005) Subcutaneous fat in normal and diseased states: 2. Anatomy and physiology of white and brown adipose tissue. J Am Acad Dermatol 53:671–683

    Article  PubMed  Google Scholar 

  2. Ramsay T (1996) Fat cells. Endocrinol Metab Clin North Am 25:847–870

    Article  PubMed  CAS  Google Scholar 

  3. Cannon B, Nedergaard J (2004) Brown adipose tissue: function and physiological significance. Physiol Rev 84:277–359

    Article  PubMed  CAS  Google Scholar 

  4. Jacobsson A, Stadler U, Glotzer MA, Kozak LP (1985) Mitochondrial uncoupling protein from mouse brown fat. Molecular cloning, genetic mapping, and mRNA expression. J Biol Chem 260:16250–16254

    PubMed  CAS  Google Scholar 

  5. Ricquier D, Casteilla L, Bouillaud F (1991) Molecular studies of the uncoupling protein. FASEB J 5:2237–2242

    PubMed  CAS  Google Scholar 

  6. Moulin K, Truel N, Andre M, Arnauld E, Nibbelink M, Cousin B et al (2001) Emergence during development of the white-adipocyte cell phenotype is independent of the brown-adipocyte cell phenotype. Biochem J 356:659

    Article  PubMed  CAS  Google Scholar 

  7. Lefterova M, Lazar M (2009) New developments in adipogenesis. Trends Endocrinol Metab 20:107–114

    Article  PubMed  CAS  Google Scholar 

  8. Gregoire F, Smas C, Sul H (1998) Understanding adipocyte differentiation. Physiol Rev 78:783–809

    PubMed  CAS  Google Scholar 

  9. Avram M, Avram A, James W (2007) Subcutaneous fat in normal and diseased states: 3. Adipogenesis: from stem cell to fat cell. J Am Acad Dermatol 56:472–492

    Article  PubMed  Google Scholar 

  10. Ntambi J, Young-Cheul K (2000) Adipocyte differentiation and gene expression. J Nutr 130:S3122–S3126

    Google Scholar 

  11. Entenmann G, Hauner H (1996) Relationship between replication and differentiation in cultured human adipocyte precursor cells. Am J Physiol Cell Physiol 270:C1011–C1016

    CAS  Google Scholar 

  12. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ et al (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228

    Article  PubMed  CAS  Google Scholar 

  13. Zuk P, Zhu M, Ashjian P, De Ugarte D, Huang J, Mizuno H et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295

    Article  PubMed  CAS  Google Scholar 

  14. Gimble J, Guilak F (2003) Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy 5:362–369

    Article  PubMed  Google Scholar 

  15. Evans C, Xie X (2008) Coherent anti-Stokes Raman scattering microscopy: chemical imaging for biology and medicine. Annu Rev Anal Chem:883–909

  16. Potma E, Xie X (2005) Direct visualization of lipid phase segregation in single lipid bilayers with coherent anti-Stokes Raman scattering microscopy. Chem Phys Chem 6:77–79

    PubMed  CAS  Google Scholar 

  17. Wang H, Fu Y, Zickmund P, Shi R, Cheng J (2005) Coherent anti-Stokes Raman scattering imaging of axonal myelin in live spinal tissues. Biophys J 89:581–591

    Article  PubMed  CAS  Google Scholar 

  18. Nan X, Cheng J, Xie X (2003) Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy. J Lipid Res 44:2202

    Article  PubMed  CAS  Google Scholar 

  19. Strem B, Hicok K, Zhu M, Wulur I, Alfonso Z, Schreiber R et al (2005) Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med 54:132–141

    Article  PubMed  CAS  Google Scholar 

  20. Pittenger M, Mackay A, Beck S, Jaiswal R, Douglas R, Mosca J et al (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147

    Article  PubMed  CAS  Google Scholar 

  21. Grigoriadis A, Heersche J, Aubin J (1988) Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone. J Cell Biol 106:2139

    Article  PubMed  CAS  Google Scholar 

  22. Johnstone B, Hering T, Caplan A, Goldberg V, Yoo J (1998) In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res 238:265–272

    Article  PubMed  CAS  Google Scholar 

  23. Saltiel A, Kahn C (2001) Insulin signalling and the regulation of glucose and lipid metabolism. Nature 414:799–806

    Article  PubMed  CAS  Google Scholar 

  24. Koopman R, Schaart G, Hesselink M (2001) Optimisation of oil red O staining permits combination with immunofluorescence and automated quantification of lipids. Histochem Cell Biol 116:63–68

    PubMed  CAS  Google Scholar 

  25. DiDonato D, Brasaemle D (2003) Fixation methods for the study of lipid droplets by immunofluorescence microscopy. J Histochem Cytochem 51:773–780

    Article  PubMed  CAS  Google Scholar 

  26. Fukumoto S, Fujimoto T (2002) Deformation of lipid droplets in fixed samples. Histochem Cell Biol 118:423–428

    Article  PubMed  CAS  Google Scholar 

  27. Zancanaro C, Carnielli V, Moretti C, Benati D, Gamba P (1995) An ultrastructural study of brown adipose tissue in pre-term human new-borns. Tissue Cell 27:339–348

    Article  PubMed  CAS  Google Scholar 

  28. Elabd C, Chiellini C, Carmona M, Galitzky J, Cochet O, Petersen R et al (2009) Human multipotent adipose-derived stem cells differentiate into functional brown adipocytes. Stem Cells 27:2753–2760

    Article  PubMed  CAS  Google Scholar 

  29. Young P, Arch J, Ashwell M (1984) Brown adipose tissue in the parametrial fat pad of the mouse. FEBS Lett 167:10–14

    Article  PubMed  CAS  Google Scholar 

  30. Casteilla L, Nougues J, Reyne Y, Ricquier D (1991) Differentiation of ovine brown adipocyte precursor cells in a chemically defined serum-free medium. Importance of glucocorticoid and age of animals. Eur J Biochem 198:195–199

    Article  PubMed  CAS  Google Scholar 

  31. Klaus S (1997) Functional differentiation of white and brown adipocytes. Bioessays 19:215–223

    Article  PubMed  CAS  Google Scholar 

  32. Cypess A, Lehman S, Williams G, Tal I, Rodman D, Goldfine A et al (2009) Identification and importance of brown adipose tissue in adult humans. N Eng J Med 360:1509–1517

    Article  CAS  Google Scholar 

  33. van Marken Lichtenbelt W, Vanhommerig J, Smulders N, Drossaerts J, Kemerink G, Bouvy N et al (2009) Cold-activated brown adipose tissue in healthy men. N Eng J Med 360:1500–1508

    Article  Google Scholar 

  34. Lean M, James W, Jennings G, Trayhurn P (1986) Brown adipose tissue in patients with pheochromocytoma. Int J Obes 10:219–227

    PubMed  CAS  Google Scholar 

  35. Tiraby C, Tavernier G, Lefort C, Larrouy D, Bouillaud F, Ricquier D et al (2003) Acquirement of brown fat cell features by human white adipocytes. J Biol Chem 278:33370–33376

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Next-Generation New-Technology Development Program of MKE, and by the Bio-signal Analysis Technology Innovation Program (No. 2009-0084137) and the National Research Foundation of Korea grant (No. 2009-0092835) of MEST, Republic of Korea.

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Authors and Affiliations

  1. Department of Dermatology, Seoul National University College of Medicine, Yongon-dong, Chongno-gu, Seoul, Korea

    Seong Jin Jo, Hyun Sun Park, Hee Chul Eun & Jin Ho Chung

  2. Wells Dermatology Clinic, 584 Shinsa-dong, Kangnam-ku, Seoul, Korea

    Won Woo Choi

  3. Center for NanoBio Convergence, Korea Research Institute of Standards and Science, Doryong 1, Yusung, Daejeon, Korea

    Eun Seong Lee, Jae Yong Lee & Dae Won Moon

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  1. Seong Jin Jo
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Correspondence to Jin Ho Chung.

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Jo, S.J., Choi, W.W., Lee, E.S. et al. Temporary Increase of PPAR-γ and Transient Expression of UCP-1 in Stromal Vascular Fraction Isolated Human Adipocyte Derived Stem Cells During Adipogenesis. Lipids 46, 487–494 (2011). https://doi.org/10.1007/s11745-011-3525-5

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  • DOI: https://doi.org/10.1007/s11745-011-3525-5

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