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Distribution of phenolic compounds and antioxidant activity between young and old leaves of Carthamus tinctorius L. and their induction by salt stress

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Abstract

In the present work, we investigated the effect of salt stress on the distribution of safflower (Carthamus tinctorius L.) antioxidant system in relation to leaf age. The study was carried out under growth chamber conditions using seedlings of three cultivars which were subjected to 0 and 50 mM NaCl for 3 weeks. Leaf growth, water content, lipid peroxidation, and phenolic compound (total polyphenols, total flavonoids, and proanthocyanidins) concentration were measured at two leaf stages (young and old leaves). Leaf growth was affected by salinity only in Kairouan cultivar that also showed a significant decrease in old leaf water content. By contrast, Gabes and Tazarka cultivars maintained their old leaf water content constant and showed a reduction in that of young leaves. This could be attributed to a higher aptitude of the latter two cultivars to use absorbed sodium and chloride for osmotic adjustment in old leaves, keeping potassium for specific functions. Salt-induced lipid peroxidation was observed only in old leaves, whereas the accumulation of the major phenolic compounds under saline conditions was higher in young leaves, except in Gabes cultivar where no significant difference was found between the two leaf stages. A significant variability was also found between the three cultivars. The better behavior of salt-challenged leaves of Gabes and Tazarka cultivars compared to that of Kairouan cultivar may be related to their higher water content and the accumulation of polyphenols, in particular flavonoids that were shown to be efficiently involved in the restriction of salt-induced oxidative damages.

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Authors and Affiliations

  1. Unité de Physiologie et de Biochimie de la Tolérance au Sel des Plantes, Faculté des Sciences de Tunis, Campus Universitaire, 2092, Tunis El Manar, Tunisia

    Saoussen Ben Abdallah, Faten Harbaoui, Mokhtar Lachâal & Najoua Karray-Bouraoui

  2. Laboratory of Extremophile Plants (LPE), Biotechnology Centre of Borj Cedria (CBBC), P.O. Box 901, 2050, Hammam-Lif, Tunisia

    Mokded Rabhi & Faten Zar-kalai

Authors
  1. Saoussen Ben Abdallah
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  2. Mokded Rabhi
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  3. Faten Harbaoui
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  4. Faten Zar-kalai
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  5. Mokhtar Lachâal
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  6. Najoua Karray-Bouraoui
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Corresponding author

Correspondence to Najoua Karray-Bouraoui.

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Communicated by J. Zwiazek.

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Abdallah, S.B., Rabhi, M., Harbaoui, F. et al. Distribution of phenolic compounds and antioxidant activity between young and old leaves of Carthamus tinctorius L. and their induction by salt stress. Acta Physiol Plant 35, 1161–1169 (2013). https://doi.org/10.1007/s11738-012-1155-z

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  • DOI: https://doi.org/10.1007/s11738-012-1155-z

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